Isolation and Functional Characterization of Multiple NADPH-Cytochrome P450 Reductase Genes from in View of Catechin Biosynthesis.

Catechins are critical constituents for the sensory quality and health-promoting benefits of tea. Cytochrome P450 monooxygenases are required for catechin biosynthesis and are dependent on NADPH-cytochrome P450 reductases (CPRs) to provide reducing equivalents for their activities. However, CPRs have not been identified in tea, and their relationship to catechin accumulation also remains unknown.

CsUGT78A15 catalyzes the anthocyanidin 3-O-galactoside biosynthesis in tea plants.

Anthocyanins are a group of natural water-soluble pigments in plants that contribute to the pink-purple color of a range of tissues. Because anthocyanins have various biological activities in human health, there is great research interest in the development of anthocyanin-rich foods and beverages, including purple shoot tea. Anthocyanidin 3-O-galactosides have been identified as one of the main anthocyanin components in purple shoot tea, but the enzyme responsible for their biosynthesis remains unclear.