Publications by authors named "Xun-Zhang Wang"

We present a case of wide-complex tachycardia in which the clinical electrophysiological diagnosis was considered to be bundle branch re-entry ventricular tachycardia. A series of ventricular entrainment attempts were performed from the left and right ventricular septum to confirm the diagnosis. Entrainment pacing with a general current output (10 mA) was performed from the right ventricular septum with manifest fusion and a post-pacing interval similar to tachycardia cycle length.

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Background: In patients with idiopathic left ventricular tachycardia (ILVT), the arrhythmogenic substrate is poorly understood.

Objective: The purpose of this study was to elucidate the ILVT characteristics and outcome of radiofrequency catheter ablation in patients with ILVT.

Methods: Twenty-four patients with ILVT and 15 patients with left accessory pathways (control) underwent high-density mapping of the left His-Purkinje system during sinus rhythm (SR) using 3-dimensional electroanatomic mapping.

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Background: To explore the association between atrial fibrillation (AF) and serum uric acid (SUA) in a general population in rural China.

Methods: From January 2013 to August 2013, we performed a cross-sectional study involving 11,956 permanent residents ≥ 35 years old in the rural Liaoning province of China. All participants completed a questionnaire, had a physical examination, and underwent an electrocardiogram (ECG) and echocardiogram.

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Article Synopsis
  • A study was conducted on 11,956 physical laborers aged 35 and older in rural Liaoning Province, China, to assess the prevalence of atrial fibrillation (AF) and its risk factors.
  • The findings showed that AF had an overall prevalence of 1.2%, which increased significantly with age, particularly in those 75 and older.
  • Key independent risk factors identified include age, diabetes, history of myocardial infarction, low left ventricular ejection fraction, and low physical activity, while factors like obesity and hypertension were not significant.
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The resolution potential of internal transcribed spacer 2 (ITS2) at deeper levels remains controversial. In this study, 105 ITS2 sequences of 55 species in Calyptratae were analyzed to examine the phylogenetic utility of the spacer above the subfamily level and to further understand its evolutionary characteristics. We predicted the secondary structure of each sequence using the minimum-energy algorithm and constructed two data matrixes for phylogenetic analysis.

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Recent studies suggest that sequence analysis technique displays a tempting foreground in identifying unknown specimens of necrophagous flies. In this study, we analyzed 63 complete ITS2 sequences concerning 29 fly species to evaluate the identification potential of the ITS2 region, among of which 41 sequence entries were obtained by sequencing and 22 sequence entries were available on the line. Additionally, phenetic method was recommended to substitute for phylogenetic method because it is very difficult to align the ITS2 sequences.

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Pentatomomorpha is the second suborder in size only to Cimicomorpha in Heteroptera. However, the phylogenetic relationships among members of the suborder are not well established. Sequences from partial nuclear ribosomal 18S gene and mitochondrial COX1 gene were analyzed separately and in combination to generate a preliminary molecular phylogeny of Pentatomomorpha based on 40 species representing 17 putative families.

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In order to produce recombinant human anti-HBsAg Fab antibody in Pichia pastoris, the recombinant yeast was fermented using fed-batch system in a 30 L bioreactor. The fermentation temperature was 30 degrees C, the pH was 5.0 approximately 5.

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Among 1,138 patients in our congestive heart failure care management database, 37 (3.2%) met guideline criteria for cardiac resynchronization therapy. Advancing age was a predictor of prolonged QRS duration in this population, but gender, ejection fraction (EF) and cause of heart failure were not.

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Objective: Despite the causes for melanin increase, the increased gene expression of TYR is a common pathological process. Based on this viewpoint, antisense-S-Oligo of TYR was designed and synthesized to regulate synthesis of melanin in order to explore the treatment for skin pigmentation.

Methods: The cultured melanocytes were divided into 3 groups.

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Pseudorabies virus glycoprotein E (PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost, a gene fragment encoding PRV gE epitopes was expressed in Pichia pastoris expression system. SDS-PAGE and Western blotting revealed that the expression product was two recombinant proteins, approximately 38 and 32 kDa, in the culture supernatant of P.

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[Baculovirus p74 gene is a species-specific gene].

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

September 2003

The p74 gene of Autographa californica multicasid nucleopolyhedrovirus (AcMNPV) bacmid was knockouted and substituted by the p74 gene of Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV), using RecA-mediated homologous recombination in the E. coli. No selection marker, which might influence the expression and function of p74 gene, was left in the modified p74 locus.

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We present an analysis of the expression profile of Schistosoma japonicum cercariae by a strategy based on expressed sequence tags (ESTs). A cDNA library from S. japoniucm cercariae was constructed and was used to generate ESTs.

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Nonrandom allelic loss on chromosome 3p is a common event in nasopharyngeal carcinoma (NPC) with the implication that certain tumor suppressor gene(s) in this region are involved in the pathogenesis of these tumors. The BLU gene, located at 3p21.3, has recently been identified as a candidate tumor suppressor gene due to the occurrence of missense mutations and loss of its expression in lung cancer.

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[High expression of LIP1 in Pichia pastoris].

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

April 2003

The gene of LIP1, the most important isoenzyme of Candida rugosa lipase (CRL), was artificially synthesized according to its mature peptide sequence. It consisted of 20 codons of preference in Pichia pastoris. The artificial gene was cloned into methanol-inducible expression vector pPICZalphaA, and constitutive expression vector pGAPZalphaA, respectively.

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Background & Objective: Nonrandom allelic loss at chromosome 3p21.3 is a common and early event in nasopharyngeal carcinoma (NPC), which implicates the presence of tumor suppressor genes (TSGs) that may be involved in the pathogenesis of NPCs. BLU gene, containing a MYND domain and located at 3p21.

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Background & Objective: Though the molecular etiology of nasopharyngeal carcinoma(NPC) is currently unknown, evidence from both loss of heterozygosity analysis and functional studies suggested that there are NPC-associated tumor suppressor genes(TSGs) residing in chromosome 3p21.3. Recently, two members of semaphorin family, SEMA3B and SEMA3F gene, located at 3p21.

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Using of two-step integrating technology, transducted the H and L chain gene of humanized Fab fragment of anti-HB-sAg antibody into the genome of methylotropic yeast P. pastoris. Constructed a engineering yeast to produce humanized Fab fragment of the anti-HBsAg antibody.

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OBJECTIVE: To understand the general profiles of the genes included in Schistosoma japonicum (Sj) cercarriae cDNA library that we have constructed for the purpose of identifying novel genes. METHODS: The phages in Sj cercariae cDNA library were transformed into plasmids after they had invaded into E.coli BM25.

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A refractile body antigen Etp28 Gene of Eimeria tenella (Guangdong Strain) sporozoites was cloned by PCR from the synthesized first strand cDNA. It has a high homology with the previously reported Etp28 gene of Merck Strain LS18. The gene was expressed through standard procedures in the modified Autographa californica nuclear polyhedrosis virus (AcMNPV-OCC(-)) expression system and large amount of heterologous fusion protein (GST-6xHis-Etp28) was obtained.

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The p10 gene was localized within the 3.0 kbp BamH I fragment of the SINPV genome. With a coding sequence of 318 nucleotides, corresponding to a protein of 105 amino acids, the p10 gene of SINPV is the longest p10 gene identified so far in NPVs.

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A 4 730 bp region of the Spodoptera litura Nucleopolyhedrovirus genome EcoRI-E fragment was sequenced, in which the odv-e66 gene and other two ORFs, ORF 1086 and the p34 gene, were found. The SpltNPV odv-e66 gene open reading frame was deduced to be 2 079 bp, encoding a protein of 692 amino acids. Unlike odv-e66 genes of AcMNPV and LsNPV, only one transcriptional initiation conserved sequence ATAAG of the late gene was present upstream of the initial codon ATG of SpltNPV odv-e66 gene, and no poly(A) signal was detected at the 3' end of the gene.

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