Copper nanocluster-Ru(dcbpy) as a cathodic ECL-RET probe combined with 3D bipedal DNA walker amplification for bioanalysis.

In this study, a cathodic intra-molecular electrochemiluminescence resonance energy transfer (ECL-RET) probe was exquisitely designed the integration of an ECL donor (Cu NCs) with an acceptor (Ru(dcbpy)), and further employed the 3D bipedal DNA walker amplification strategy to monitor the platelet-derived growth factor BB (PDGF-BB). Specifically, blue emission Cu NCs with low consumption, biocompatibility and numerous resources, act as well-overlapped donors and significantly improve the ECL efficiency of Ru(dcbpy). More impressively, the intra-molecular ECL-RET of Cu NC-Ru endowed a better and more stable ECL signal by reducing the electron-transfer distance and decreasing the energy loss.

Core-shell Au@PtAg modified TiO-TiC heterostructure and target-triggered DNAzyme cascade amplification for photoelectrochemical detection of ochratoxin A.

Efficient charge separation and utilization are critical factors to obtain a high initial signal in photoelectrochemical (PEC) aptasensor. Reports demonstrate that constructing metal/semiconductor Schottky junction can effectively improve the charge separation efficiency. Herein, a photoelectrode Au@PtAg/TiO-TiC Schottky junction is successfully synthesized.

DNA coated CoZn-ZIF metal-organic frameworks for fluorescent sensing guanosine triphosphate and discrimination of nucleoside triphosphates.

Imidazole-based metal-organic frameworks (MOFs) are easy to prepare as well-dispersed nanoparticles, which have attracted a lot of interest in sensing. Metal substitution is an effective way to regulate the composition and performance of MOFs. Herein, by tuning the contents of Co and Zn, a series of homobimetallic CoZn-ZIF (x = 0-100) were synthesized.

Cu(I)-Catalyzed Click Reaction-Triggered 3D DNA Walker for Constructing an "OFF-ON" Fluorescent Biosensor for Cu Detection.

Herein, a highly selective and sensitive "OFF-ON" fluorescent biosensor was designed for intracellular Cu detection. Compared to the fluorescent Cu biosensors reported so far, this work tackled the tricky issue of reliability of Cu, which mainly depends on the integration of the high selectivity of the Cu(I)-catalyzed click reaction with the ultrahigh sensitivity of a spherical nucleic acid-based 3D DNA walker. Typically, DNA track is carried out by coconjugating N-S1 and Cy3-HP onto gold nanoparticles (AuNPs).

A sensor array based on DNA-wrapped bimetallic zeolitic imidazolate frameworks for detection of ATP hydrolysis products.

Most current biosensors were designed for the detection of individual analytes, or a group of chemically similar analytes. We reason that sensors designed to track both reactants and products might be useful for following chemical reactions. Adenosine triphosphate (ATP) is a key biomolecule that participates in various biochemical reactions, and its hydrolysis plays a fundamental role in life.

Sensing ATP: Zeolitic Imidazolate Framework-67 Is Superior to Aptamers for Target Recognition.

In a typical biosensor, a biomolecule such as an aptamer is used for target recognition, and a nanomaterial is used for signal generation. Herein, we communicate a reverse system using a nanomaterial for target recognition and a DNA for signaling. We discovered that a classic metal-organic framework material, zeolitic imidazolate framework (ZIF)-67, has ultrahigh selectivity for recognizing adenosine triphosphate (ATP), allowing a fluorescently labeled DNA oligonucleotide to be used for signal generation.

Cortex dictamni-induced liver injury in mice: The role of P450-mediated metabolic activation of furanoids.

Many furan containing compounds have been reported to be toxic resulted from the metabolic activation of the furan ring to reactive metabolite (RM). Cortex Dictamni (CD), a widely used herbal medicine, has been reported to cause severe even fatal hepatotoxicity. The injurious components and mechanism of CD-induced liver injury remain unclear.

Simultaneous Quantitative Determination of Polyphenolic Compounds in (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector.

A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of , including 17 flavonoids and 1 phenylethanone. The extraction was separated by a Kromasil C column (250 × 4.6 mm, 5 m) with a binary gradient mobile phase consisting of acetonitrile and 0.

Ultrasensitive aptasensing of insulin based on hollow porous CN/SO/AuPtAg ECL ternary system and DNA walker amplification.

In this work, a high-efficiency electrochemiluminescence (ECL) ternary system was constructed for ultrasensitive assay of insulin based on hollow porous graphitic carbon nitride (HP-CN) as novel luminophore, SO as coreactant and tri-metallic AuPtAg as coreaction accelerator. Specifically, in comparison with CN-based bulk nanomaterials, the as-prepared HP-CN exhibits high luminous efficiency though decreased inner filter effect and minimized inactive ECL emitter. Noteworthy, tri-metallic AuPtAg, possessing the superiority of Au, Pt and Ag, was first used as coreaction accelerator to significantly enhance ECL intensity of HP-CN and SO.

Gypenosides Altered Hepatic Bile Acids Homeostasis in Mice Treated with High Fat Diet.

Gypenosides extracted from (Thunb.) Makino have significant role in reducing serum lipid level and treating fatty liver diseases, however, without clear mechanism. As gypenosides share the similar core structures with bile acids (the endogenous ligands of nuclear receptor FXR), we hypothesize that gypenosides may improve hypercholesterolemia via FXR-mediated bile acids signaling.

Development and validation of a rapid LC-MS/MS method for simultaneous quantification of arecoline and its two active metabolites in rat plasma and its application to a pharmacokinetic study.

Arecoline is the primary active and toxic constituent of areca nut. Arecaidine and arecoline N-oxide are two major active metabolites of arecoline. In this work, an accurate and simple high performance liquid chromatography tandem mass spectrometry method for simultaneous quantification of arecoline, arecaidine and arecoline N-oxide in rat plasma was developed and fully validated to study their pharmacokinetic behaviors in rats.

Liquid chromatography-tandem mass spectrometric assay for determination of unstable arecoline in rat plasma and its application.

Arecoline, a predominant alkaloid in areca nut, shows several pharmacological and toxicological effects. In present study, a sensitive and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and fully validated for quantification of arecoline in rat plasma. Importantly, our group found that arecoline was highly unstable in rat plasma samples, which brought challenges to accurately quantify in vivo.

Separation and purification of five alkaloids from Aconitum duclouxii by counter-current chromatography.

C19 -diterpenoid alkaloids are the main components of Aconitum duclouxii Levl. The process of separation and purification of these compounds in previous studies was tedious and time consuming, requiring multiple chromatographic steps, thus resulted in low recovery and high cost. In the present work, five C19 -diterpenoid alkaloids, namely, benzoylaconine (1), N-deethylaconitine (2), aconitine (3), deoxyaconitine (4), and ducloudine A (5), were efficiently prepared from A.

Separation and purification of 9,10-dihydrophenanthrenes and bibenzyls from Pholidota chinensis by high-speed countercurrent chromatography.

Stilbenoids are the main components of leaves and stems of Pholidota chinensis. In the present investigation, high-speed counter-current chromatography was used for the separation and purification of two classes of stilbenoids, namely, bibenzyls and 9,10-dihydrophenanthrenes, on a preparative scale from whole plants of P. chinensis with different solvent systems after silica gel column chromatography fractionation.