Customizable hydrospongel based on chitosan microfibers.

Customizable and viscoelastic porous biomaterials are highly desired as implant scaffold for repairing large-volume defects. Herein, we report customizable chitosan microfibers (CMFs)-based hydrospongels with mechanical properties comparable to soft tissues. The CMFs formed under high-speed shearing during crystallization of chitosan, and then they are crosslinked through covalent bond and hydrogen bond to form hydrospongels.

Recombinant characterization and pathogenicity of a novel L1C RFLP-1-4-4 variant of porcine reproductive and respiratory syndrome virus in China.

Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant diseases affecting the pig industry worldwide and is caused by the PRRS virus (PRRSV), which has complex genetic variation due to frequent mutations, indels, and recombination. The emergence of PRRSV L1C.5 in 2020 in the United States has raised worldwide concerns about PRRSV with the RFLP 1-4-4 pattern and lineage 1C.

Polydopamine-modified konjac glucomannan scaffold with sustained release of vascular endothelial growth factor to promote angiogenesis.

The fabrication of scaffolds capable of the sustained release of the vascular endothelial growth factor (VEGF) to promote angiogenesis for a long time remains a challenge in tissue engineering. Here, we report a facile approach for effectively fabricating a bioactive scaffold that gradually releases VEGF to promote angiogenesis. The scaffold was fabricated by coating polydopamine (PDA) on a konjac glucomannan (KGM) scaffold, followed by the surface immobilization of VEGF with PDA.

Effect of HER2 expression status on the prognosis of patients with HR/HER2 advanced breast cancer undergoing advanced first‑line endocrine therapy.

The present study aimed to retrospectively assess the effects of human epidermal growth factor receptor 2 (HER2) expression on the diagnosis of patients with hormone receptor (HR)/HER2 late-stage breast cancer undergoing advanced first-line endocrine-based treatment. A total of 72 late-stage breast tumor cases from June 2017 to June 2019 were selected from the Department of Surgical Oncology, Shaanxi Provincial People's Hospital (Xi'an, China) and included in the present study. The expression of estrogen receptor, progesterone receptor and HER2 was detected by immunohistochemistry.

Immunomodulatory role of mesenchymal stem cell therapy in liver fibrosis.

Liver fibrosis is a fibrogenic and inflammatory process that results from hepatocyte injury and is characterized by hepatic architectural distortion and resultant loss of liver function. There is no effective treatment for advanced fibrosis other than liver transplantation, but it is limited by expensive costs, immune rejection, and postoperative complications. With the development of regenerative medicine in recent years, mesenchymal stem cell (MSCs) transplantation has become the most promising treatment for liver fibrosis.

Nanocellulose-based hydrogels as versatile drug delivery vehicles: A review.

Hydrogels designed with nanocellulose (i.e. cellulose nanocrystals (CNC), cellulose nanofibrils (CNF), and bacterial cellulose (BC)) have significant advantages as drug carriers due to their environmentally-benign features and excellent properties.

Biological Application of Novel Biodegradable Cellulose Composite as a Hemostatic Material.

Degradable hemostatic materials have unique advantages in reducing the amount of bleeding, shortening the surgical operation time, and improving patient prognosis. However, none of the current hemostatic materials are ideal and have disadvantages. Therefore, a novel biodegradable cellulose-based composite hemostatic material was prepared by crosslinking sodium carboxymethyl cellulose (CCNa) and hydroxyethyl cellulose (HEC), following an improved vacuum freeze-drying method.

Construction of a Prognostic Nomogram Model for Patients with Mucinous Breast Cancer.

Objective: The objective of the study is to develop a nomogram for estimating three- and five-year survival rates in mucinous breast cancer patients.

Methods: Between 2010 and 2016, the National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) were searched as a data source for patients associated with mucinous breast cancer (MBC). A total of 3964 patients were recruited after screening.

Mesenchymal Stem Cells (MSCs) in Targeted Drug Delivery: Literature Review and Exploratory Data on Migrating and Differentiation Capacities of Bone MSCs into Hepatic Progenitor Cells.

Background And Objective: Mesenchymal stem cells (MSCs), particularly bone MSCs (BMSCs) offer great potentials for targeted therapeutic applications owing to their migratory and differentiation capacities. Significant advances have been achieved in the differentiation of hepatocyte or hepatocyte-like cells both in vitro and in vivo. However, there is limited knowledge on the differentiation of BMSCs into bipotential hepatic progenitor cells or cholangiocyte.

Doxorubicin-loaded FeO-ZIF-8 nano-composites for hepatocellular carcinoma therapy.

Hepatocellular carcinoma (HCC) is one of the most common and malignant cancers and has no effective therapeutic approaches. Chemotherapeutic drug doxorubicin (DOX) is widely used for HCC therapy, but its application is limited by the clinical toxicity. In the present study, an FeO-ZIF-8 magnetic nano-composite was fabricated and used for DOX delivery for HCC therapy.

Association analysis of telomere length related gene ACYP2 with the gastric cancer risk in the northwest Chinese Han population.

Gastric cancer (GC) is a complex multifactorial disease, and genetic factors are believed the predominant cause to the occurrence of GC. We sought to investigate the associations between single nucleotide polymorphisms (SNPs) in ACYP2 gene and the risk of GC in the Northwest Chinese Han population. We recruited 302 GC cases and 300 controls from northwest China and selected 13 SNPs from ACYP2 gene.

[Strategies to choose scaffold materials for tissue engineering].

Current therapies of organ failure or a wide range of tissue defect are often not ideal. Transplantation is the only effective way for long time survival. But it is hard to meet huge patients demands because of donor shortage, immune rejection and other problems.

Liver regeneration using decellularized splenic scaffold: a novel approach in tissue engineering.

Background: The potential application of decellularized liver scaffold for liver regeneration is limited by severe shortage of donor organs. Attempt of using heterograft scaffold is accompanied with high risks of zoonosis and immunological rejection. We proposed that the spleen, which procured more extensively than the liver, could be an ideal source of decellularized scaffold for liver regeneration.

[Optimization of the protocols for in vitro culture and induction of hepatic differentiation of rat mesenchymal stem cells].

Objective: To optimize the protocols for isolation, in vitro culture, identification and induction of hepatic differentiation of rat bone marrow mesenchymal stem cells (BMSCs).

Methods: Rat BMSCs were separated and purified by differential adherent culture for 1.5 h with the first medium change at 12 h.

[Optimization of the method for isolating and culturing rat mesenchymal stem cells].

Objective: To optimize the protocols for isolation and culture of mesenchymal stem cells from rat bone marrow (BMSCs).

Methods: BMSCs were isolated by adherence to plastic with frequent medium change and reduced trypsinization time. The cell growth curves were drawn and the surface markers of BMSCs were detected by flow cytometry.