Overexpression of SLC2A1, ALDOC, and PFKFB4 in the glycolysis pathway drives strong drug resistance in 3D HeLa tumor cell spheroids.

The 3D multicellular tumor spheroid (MTS) model exhibits enhanced fidelity in replicating the tumor microenvironment and demonstrates exceptional resistance to clinical drugs compared to the 2D monolayer model. In this study, we used multiomics (transcriptome, proteomics, and metabolomics) tools to explore the molecular mechanisms and metabolic differences of the two culture models. Analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathways revealed that the differentially expressed genes between the two culture models were mainly enriched in cellular components and biological processes associated with extracellular matrix, extracellular structural organization, and mitochondrial function.

[Effects of moso bamboo () expansion on soil microbial community in evergreen broad-leaved forest].

The special eco-physiological characteristics of moso bamboo () facilitate their fast invasion in nature ecosystems. The widespread expansion of moso bamboo causes degradation of adjacent forest ecosystem and change of landscape, as well as soil properties and microbial community composition. However, how moso bamboo expansion affects soil microbial composition is far from fully understood.

Land-Use Change Enhanced SOC Mineralization but Did Not Significantly Affect Its Storage in the Surface Layer.

To achieve carbon (C) neutrality and mitigate climate change, it is crucial to understand how converting natural forests to agricultural plantations influences soil organic C (SOC) mineralization. In this study, we investigated the impact of converting evergreen broadleaf forests (EBF) to extensively managed Moso bamboo ( (Carriere) J. Houzeau) plantations (MBP) in subtropical China on SOC mineralization rate; the concentrations of labile SOC fractions such as dissolved organic C (DOC), microbial biomass C (MBC), and readily oxidizable C (ROC); the activities of C-degrading enzymes (cellobiohydrolase and phenol oxidase); and the abundance of C-degrading enzyme-encoding genes (I and ).

[Research on retrieving thermospheric O/N2 from FUV remote sensing].

Magnetic storms usually cause significant departures of thermospheric O and N2 from their normal values. To study the effects on thermospheric neutral species caused by magnetic storms, a method to retrieve thermospheric O/N2 based on the data obtained from global ultraviolet imager on board TIMED is presented. With the help of AURIC, the normalizations of observing angles and SZAs were preformed to the measurements and a relationship between 135.