High-throughput screening of novel TFEB agonists in protecting against acetaminophen-induced liver injury in mice.

Macroautophagy (referred to as autophagy hereafter) is a major intracellular lysosomal degradation pathway that is responsible for the degradation of misfolded/damaged proteins and organelles. Previous studies showed that autophagy protects against acetaminophen (APAP)-induced injury (AILI) selective removal of damaged mitochondria and APAP protein adducts. The lysosome is a critical organelle sitting at the end stage of autophagy for autophagic degradation fusion with autophagosomes.

Identification, flavor characteristics and molecular docking of umami taste peptides of Xuanwei ham.

To better understand the palatable properties of Xuanwei ham, the aqueous extract was isolated, analyzed and combin with sensory evaluation. Of umami-tasting activity and umami-enhancing impact, four new peptides (MDAIKKMQ, RKYEEVAR, YVGDEAQSKRG, and VNVDEVGGEALGR) were extracted and identified by ultrafiltration, gel separation, reverse performance liquid chromatography, and nano-LC-MS / MS. Sensory evaluation results showed that all of them had umami activity and enhanced umami taste, among which VNVDEVGGEALGR had the best effect.

Flame atomic absorption spectrometry combined with surface-modified magnetic mesoporous silica microspheres by polyethyleneimine for enrichment, isolation and determination of Cu in preserved eggs after high-temperature digestion.

A new efficient magnetic solid-phase extractant based on a surface-modified magnetic mesoporous silica microsphere referred as MMSM-PEI was synthesised and used for the enrichment and isolation of copper ions (Cu) in preserved eggs. The physicochemical properties and morphology of MMSM-PEI were characterized by X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), vibration sample magnetometry (VSM), scanning electron microscopy (SEM) and thermos-gravimetric analyses (TGA). The concentrations of trace Cu in the preserved egg were determined by flame atomic absorption spectroscopy (FAAS).

Effective preparation of magnetic molecularly imprinted polymer nanoparticle for the rapid and selective extraction of cyfluthrin from honeysuckle.

Cyfluthrin is a widely used pesticide. In this study, a sensitive and efficient magnetic molecularly imprinted polymer (MMIP) was prepared by surface molecular imprinting, which used functionalized FeO particles as magnetic cores. Cyfluthrin was extracted and enriched using magnetic molecularly polymer for analyzing pesticide residue of Chinese herbal medicines.

Magnetic molecularly imprinted polymer combined with high performance liquid chromatography for selective extraction and determination of the metabolic content of quercetin in rat plasma.

In this study, a novel magnetic solid phase extraction adsorbent was prepared, namely FeO@SiO as the carrier, quercetin as the template molecule, acrylamide (AM) as the functional monomer, ethylene glycol dimethacrylamide (EGDMA) as the crosslinker, 2,2'-azoisobutyronitrile (AIBN) as the initiator to prepare magnetic molecularly imprinted polymer (MMIPs) with specific adsorption of quercetin by surface molecular imprinting technique. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and transmission electron microscope (TEM). The binding properties of MMIPs were evaluated by isothermal adsorption, kinetic adsorption and selective adsorption experiments.

Four new compounds from .

Four new compounds, compounds , , , , along with two known compounds , , were isolated from the methanol extract of the fruiting body of . The structures of compounds were elucidated by HRMS and NMR spectroscopic methods. The anti-inflammatory activity of the isolated compounds was evaluated.

Draft Genome Sequence of Fungus Clonostachys rosea Strain YKD0085.

Here, we report the draft genome sequence of Clonostachys rosea (strain YKD0085). The functional annotation of C. rosea provides important information related to its ability to produce secondary metabolites.

A Rapid Centrifugation-Assisted Solid-Phase Extraction and Liquid Chromatography Method for Determination of Loureirin A and Loureirin B of Dragon's Blood Capsules in Rat Plasma and Urine After Oral Administration.

A simple, sensitive and rapid centrifugation-assisted solid-phase extraction (SPE) with high-performance liquid chromatography (SPE-HPLC) method was developed for simultaneous determination of the metabolites loureirin A and loureirin B from Dragon's blood in rat plasma and urine. The development of the extraction procedure included optimization of some important extraction phases. After evaluation, the metabolites of Dragon's blood were extracted by centrifugation-assisted SPE and separated by using HPLC.

Utility of 4-chloro-7-nitrobenzofurazan for the spectrofluorimetric determination of butylated hydroxyanisole and propyl gallate in foodstuffs.

Unlabelled: A spectrofluorimetric method is presented for the determination of 2 synthetic phenolic antioxidants (SPAs), butylated hydroxyanisole (BHA), and propyl gallate (PG) in foodstuffs. The proposed method is based on the derivatization of SPAs with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in phosphate buffer of pH 9.0 to yield a highly fluorescent brown product.

Three new indole alkaloids from Trigonostemon lii.

Three unprecedented indole alkaloids, trigonoliimines A-C (1-3) with a unique polycyclic system, were isolated from the leaves of Trigonostemon lii Y. T. Chang.

Carboline alkaloids from Trigonostemon lii.

Three types with six new carboline alkaloids, trigonostemonines A-F (1- 6), were isolated from the roots and stems of Trigonostemon lii. Their structures were elucidated by spectroscopic analyses. It is the first time that beta-carboline alkaloids have been reported in Trigonostemon species.

Two new norbisabolane sesquiterpenoid glycosides from Glochidion coccineum.

Two new norbisabolane sesquiterpenoid glycosides, glochicoccinosides A (1) and B (2), together with two known compounds, have been isolated from the rhizomes of Glochidion coccineum. Their structures were elucidated by the combination of 1D NMR, 2D NMR, and MS spectral analysis, as well as chemical evidence. Cytotoxic activities and the antioxidant effect of these compounds were evaluated, but none of them showed activity.

Tarennane and tarennone, two novel chalcone constituents from Tarenna attenuata.

Two novel chalcone constituents, tarennane (1) and tarennone (2), together with nine known compounds, were isolated from the whole plant of Tarenna attenuata. By analysis of physical and spectroscopic data, the structures of new compounds were elucidated as (E)-4-[3-(4-hydroxy-3-methoxyphenyl)-acryl]-3,4,5-trimethoxycyclohexa-2,5-dienone (1) and 1,2-bis(3,5-dimethoxy-4-hydroxyphenyl)-3-hydroxypropan-1-one (2). These two compounds were tested for antioxidant activities in the MTT and DPPH assays.