Ultralow Dose Iron-Copper Bimetallic Single-Atom Nanozymes for Efficient Photothermal-Chemodynamic Antibacterial and Wound Healing.

The combination of photothermal and chemodynamic therapy (PTT-CDT) using single-atom nanozymes (SAzymes) shows great promise in combating pathogenic and drug-resistant bacteria. However, the photothermal conversion efficiency and catalytic activity of SAzymes with solely metal sites remain inadequate, often requiring high doses for effectiveness. Herein, a bimetallic single-atomic nanozymes with Fe and Cu active sites (FeCu BSNs) designed is reported for efficient treatment of bacterial infections through hyperthermia-amplified nanozyme catalysis strategy.

CLK2 Condensates Reorganize Nuclear Speckles and Induce Intron Retention.

Intron retention (IR) constitutes a less explored form of alternative splicing, wherein introns are retained within mature mRNA transcripts. This investigation demonstrates that the cell division cycle (CDC)-like kinase 2 (CLK2) undergoes liquid-liquid phase separation (LLPS) within nuclear speckles in response to heat shock (HS). The formation of CLK2 condensates depends on the intrinsically disordered region (IDR) located within the N-terminal amino acids 1-148.

Identification and Validation of a PEX5-Dependent Signature for Prognostic Prediction in Glioma.

Gliomas, the most prevalent and lethal form of brain cancer, are known to exhibit metabolic alterations that facilitate tumor growth, invasion, and resistance to therapies. Peroxisomes, essential organelles responsible for fatty acid oxidation and reactive oxygen species (ROS) homeostasis, rely on the receptor PEX5 for the import of metabolic enzymes into their matrix. However, the prognostic significance of peroxisomal enzymes for glioma patients remains unclear.

A novel EGFR variant EGFRx maintains glioblastoma stem cells through STAT5.

Background: Glioblastomas are universally lethal brain tumors containing tumor-propagating glioblastoma stem cells (GSCs). EGFR gene amplification or mutation is frequently detected in GBMs and is associated with poor prognosis. However, EGFR variants in GSCs and their role in the maintenance of GSCs and progression of GBM are unclear.

Single atomic cerium sites anchored on nitrogen-doped hollow carbon spheres for highly selective electroreduction of nitric oxide to ammonia.

Electrocatalytic nitric oxide reduction reaction (NORR) at ambient environments not only offers a promising strategy to yield ammonia (NH) but also degrades the NO contaminant; however, its application depends on searching for high-performance catalysts. Herein, we present single atomic Ce sites anchored on nitrogen-doped hollow carbon spheres that are capable of electro-catalyzing NO reduction to NH in an acidic solution, achieving a maximal Faradaic efficiency of 91 % and a yield rate of 1023 μg h mg at -0.7 V vs RHE for NH formation, both of which outperform these on Ce nanoclusters and approach the best-reported results.

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells.

Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-).

Peroxisomal Membrane Contact Sites in Mammalian Cells.

Peroxisomes participate in essential cellular metabolic processes, such as oxidation of fatty acids (FAs) and maintenance of reactive oxygen species (ROS) homeostasis. Peroxisomes must communicate with surrounding organelles to exchange information and metabolites. The formation of membrane contact sites (MCSs), where protein-protein or protein-lipid complexes tether the opposing membranes of two organelles, represents an essential means of organelle crosstalk.

Knockout of the Placenta Specific 8 Gene Affects the Proliferation and Migration of Human Embryonic Kidney 293T Cell.

Candidate oncogene placenta specific 8 (PLAC8) has been identified to participate in different cellular process and human diseases. However, the effects of PLAC8 on cell proliferation and migration in human kidney cancer (KC) remained unclear. In current study, physiological effects of PLAC8 in immortalized human embryonic kidney cell line (HEK293T) were investigated in vitro.

Placenta-specific 9, a putative secretory protein, induces G2/M arrest and inhibits the proliferation of human embryonic hepatic cells.

Placenta-specific 9 () is a putative secreted protein that was first discovered in the context of embryogenesis. The expression pattern of during embryogenesis, together with the results of recent reports, suggest that may play a role in the liver development. The present study was conducted to investigate the secretory characteristics of and its potential role in liver cell physiology.

Probing Phase Evolutions of Au-Methyl-Propyl-Thiolate Self-Assembled Monolayers on Au(111) at the Molecular Level.

A self-assembled monolayer (SAM) consisting of a mixture of CHS-Au-SCH, CHS-Au-S(CH)CH, and CH(CH)S-Au-S(CH)CH was studied systematically using scanning tunneling microscopy and density functional calculations. We find that the SAM is subjected to frequent changes at the molecular level on the time scale of ∼minutes. The presence of CHS or CHS-Au as a dissociation product of CHS-Au-SCH plays a key role in the dynamical behavior of the mixed SAM.