Greenhouse gases emissions from aquaculture ponds: Different emission patterns and key microbial processes affected by increased nitrogen loading.

Global aquaculture production is expected to rise to meet the growing demand for food worldwide, potentially leading to increased anthropogenic greenhouse gases (GHG) emissions. As the demand for fish protein increases, so will stocking density, feeding amounts, and nitrogen loading in aquaculture ponds. However, the impact of GHG emissions and the underlying microbial processes remain poorly understood.

Exploring organic matter conversion pathway and its effect on nitrogen removal in tidal flow constructed wetlands.

Achieving effective nitrogen removal remains a significant challenge faced by constructed wetlands. Although organic matter is a crucial factor influencing nitrogen removal, little attention has been paid to the impact of organic matter conversion pathways on nitrogen removal in constructed wetlands. Here, we showed that endogenous microorganisms performing carbon internalization could be easily enriched in tidal flow constructed wetlands (TFCWs) under its special rhythmic cycle of anaerobic/aerobic operational mode.

Selenium attenuated food borne cadmium-induced intestinal inflammation in red swamp crayfish (Procambarus clarkii) via regulating PI3K/Akt/NF-κB pathway.

Selenium (Se), an indispensable micronutrient for living organisms, has been extensively studied for its heavy metal-detoxifying properties in diverse biological systems and tissues. Nevertheless, it is not entirely certain whether Se can effectively protect against Cadmium (Cd)-induced gut inflammation, especially in aquatic animals. In this study, we employed various approaches, including transcriptome profiling, histological examinations, assessment of antioxidant enzyme activities, and analysis of gut microbiota composition to investigate the effects on crayfish growth and intestinal health after exposure to dietary Cd (15 mg kg diet) and Se (15 mg kg diet) individually or in combination for 8 weeks.

The effect of novel aquaculture mode on phosphorus sorption-release in pond sediment.

The emergence of aquaculture modes has brought considerable changes to the aquaculture landscape and profoundly influenced environmental processes. However, there is limited research on nutrient cycling in emerging aquaculture modes. This study investigated the characteristics and mechanisms of sediment phosphorus (P) sorption-release in traditional earthen pond culture (TEP) and pond-tank culture mode (PTC), which represents novel aquaculture modes.

Mangiferin and Taurine Ameliorate MSRV Infection by Suppressing NF-κB Signaling.

The emergence or reemergence of viruses pose a substantial threat and challenge to the world population, livestock, and wildlife. However, the landscape of antiviral agents either for human or animal viral diseases is still underdeveloped. The far tougher actuality is the case that there are no approved antiviral drugs in the aquaculture industry, although there are diverse viral pathogens.

BJTJ-1837, a novel FXI activation-blocking antibody.

Background: Factor (F)XI contributes to thrombosis development while it plays a limited role in normal hemostasis. FXI targeting has the potential for preventing and treating thrombosis with little bleeding risk.

Objectives: The aim of this study was to develop novel antibody therapeutics against FXI for the treatment of thrombosis-related diseases.

Formation of microorganism-derived dissolved organic nitrogen in intermittent aeration constructed wetland and its stimulating effect on phytoplankton production: Implications for nitrogen mitigation.

To control eutrophication in aquatic ecosystems, enhancing nitrogen removal in the constructed wetland (CW) by upgrading conventional CW to aeration CW is commonplace. However, regulatory efforts have only focused on reducing dissolved inorganic nitrogen (DIN) discharge and disregarding dissolved organic nitrogen (DON). Here, we used experimental mesocosms to investigate the effect of aeration mode on the characteristics of effluent DON in CW.

Sediment diffusion is feasible to simultaneously reduce nitrate discharge from recirculating aquaculture system and ammonium release from sediments in receiving intensive aquaculture pond.

Nitrogen accumulation has become one of the greatest unresolved challenges restricting the development of aquaculture worldwide. In recirculating aquaculture system (RAS), lack of organic matter (OM) and sensitive organisms makes it difficult to apply efficient denitrifying technology, thus leading to a high nitrate‑nitrogen (NO-N) accumulation. In contrast, excess OM accumulation in intensive aquaculture pond sediments is associated with dissolved oxygen depletion and ammonium‑nitrogen (NH-N) accumulation in the sediments.

Isoandrographolide from Andrographis paniculata ameliorates tubulointerstitial fibrosis in ureteral obstruction-induced mice, associated with negatively regulating AKT/GSK-3β/β-cat signaling pathway.

Tubulointerstitial fibrosis (TIF) is a prominent pathological manifestation for the progression of almost all chronic kidney diseases (CKDs) to end-stage renal failure. However, there exist few efficient therapies to cure TIF. Our recent results showed that (8R, 12S)-isoandrographolide (ISA), a diterpenoid lactone ingredient of traditional Chinese herbal Andrographis paniculata (Burm.

Integrating Microbial Protein Production and Harvest Systems into Pilot-Scale Recirculating Aquaculture Systems for Sustainable Resource Recovery: Linking Nitrogen Recovery to Microbial Communities.

In aquaculture, it is important to raise the nitrogen recovery efficiency (NRE) to improve sustainability. To achieve this, recovery of microbial protein (RMP), instead of nitrification/denitrification in conventional wastewater treatment, is a promising approach whose microbiological mechanisms must be characterized. Here, periodic RMP was conducted in an biofloc-based aquaculture system (IBAS) and a separating assimilation reactor-based recirculating aquaculture system (SRAS).

Strengthening anoxic glycogen consumption in SNEDPR-CW as a strategy to control PAO-GAO competition under carbon limited condition.

Cooperation between Phosphate and Glycogen Accumulating Organisms (PAOs and GAOs) plays a pivotal role in nutrients removal in simultaneous nitrification endogenous denitrification and phosphorous removal (SNEDPR) systems. Recent findings have expanded the application of SNEDPR from activated sludge system to constructed wetland (CW). However, how to regulate competition between PAOs and GAOs in SNEDPR-based CW is still unclear.

Advanced nitrogen and phosphorus removal by combining endogenous denitrification and denitrifying dephosphatation in constructed wetlands.

To achieve high-efficiency nutrient removal in constructed wetlands (CWs), a novel simultaneous nitrogen and phosphorus removal (SNPR) process was developed by combining nitrification, endogenous denitrification, and denitrifying phosphorus removal. In SNPR process, denitrifying glycogen-accumulating organisms (DGAOs) and denitrifying polyphosphate-accumulating organisms (DPAOs) utilized NO-N(NO-N or NO-N) as electron acceptor and poly-beta-hydroxy-alkanoates (PHAs) as carbon sources for endogenous denitrification and denitrifying phosphorus removal processes. Results from 217 days of operation showed that a high-level of nitrogen removal efficiency of 83.

Dynamic changes in microbial community structure in farming pond water and their effect on the intestinal microbial community profile in juvenile common carp (Cyprinus carpio L.).

Water quality parameter dynamics, gut, sediment and water bacteria communities were studied to understand the environmental influence on the gut microbial community of a new strain of Huanghe common carp. A total of 3,384,078 raw tags and 5105 OTUs were obtained for the gut, water and sediment bacteria. The water quality had a stronger influence on the water bacteria community than gut and sediment bacteria communities.

Nitrogen removal by heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas sp. DM02: Removal performance, mechanism and immobilized application for real aquaculture wastewater treatment.

A bacterial strain was isolated and identified as Pseudomonas sp. DM02 from an aquaculture system. Strain DM02 showed efficient heterotrophic nitrification-aerobic denitrification capability.

Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio.

In this study, the effect of aerobic denitrification on nitrogen removal was investigated using two zero-discharge biofloc-based recirculating aquaculture systems with representative carbon-to-nitrogen (C/N) ratios of 15 (CN15) and 20 (CN20). Aquaculture wastewater, residual feed, and fish feces were treated in an aerated suspended growth reactor (SGR, dissolved oxygen > 5.0 mg L).

Improvement of Muscle Quality of Grass Carp () With a Bio-Floating Bed in Culture Ponds.

Muscle quality and the physiological condition of fish are affected greatly by the culture environment. In aquaculture ponds, a bio-floating bed planted with vegetation is often used to improve water quality. This study investigated the growth and muscle quality of grass carp () cultured in ponds equipped with bio-floating beds.

Diet Affects Muscle Quality and Growth Traits of Grass Carp (): A Comparison Between Grass and Artificial Feed.

Fish muscle, the main edible parts with high protein level and low fat level, is consumed worldwide. Diet contributes greatly to fish growth performance and muscle quality. In order to elucidate the correlation between diet and muscle quality, the same batch of juvenile grass carp () were divided into two groups and fed with either grass ( and ) or artificial feed, respectively.

Enhancing Nitrate Removal from Freshwater Pond by Regulating Carbon/Nitrogen Ratio.

Nitrogen accumulation is a serious environmental problem in freshwater ponds, which can lead to massive death of fish and shrimps as well as the eutrophication. The removal of nitrate by regulating the carbon to nitrogen (C/N) ratio and the underlying mechanisms were investigated. The nitrate removal system comprised 530-mL medium containing 5 mg/L [Formula: see text]-N and 0-66.

Achieving short-cut nitrification and denitrification in modified intermittently aerated constructed wetland.

This study aim to enhance nitrogen removal performance via shifting nitrogen removal pathway from nitrate to nitrite pathway. It was demonstrated that nitrite pathway was successfully and stably achieved in CWs by using modified intermittent aeration control with aeration 20min/non-aeration 100min and reducing DO concentration during aeration, nitrite in the effluent could accumulate to over 70% of the total oxidized nitrogen. Q-PCR analysis showed that nitrifying microbial communities were optimized under the alternating anoxic and aerobic conditions, ammonia oxidizing bacteria increased from 7.

Mercury bioaccumulation in the food web of Three Gorges Reservoir (China): Tempo-spatial patterns and effect of reservoir management.

Tempo-spatial patterns of mercury bioaccumulation and tropho-dynamics, and the potential for a reservoir effect were evaluated in the Three Gorges Reservoir (TGR, China) from 2011 to 2012, using total mercury concentrations (THg) and stable isotopes (δ(13)C and δ(15)N) of food web components (seston, aquatic invertebrates and fish). Hg concentrations in aquatic invertebrates and fish indicated a significant temporal trend associated with regular seasonal water-level manipulation. This includes water level lowering to allow for storage of water during the wet season (summer); a decrease of water levels from September to June providing a setting for flood storage.

Bulk signatures of pressure-induced band inversion and topological phase transitions in Pb(1-x)Sn(x)Se.

The characteristics of topological insulators are manifested in both their surface and bulk properties, but the latter remain to be explored. Here we report bulk signatures of pressure-induced band inversion and topological phase transitions in Pb(1-x)Sn(x)Se (x=0.00, 0.

Engineering integrable nonautonomous nonlinear Schrödinger equations.

We investigate Painlevé integrability of a generalized nonautonomous one-dimensional nonlinear Schrödinger (NLS) equation with time- and space-dependent dispersion, nonlinearity, and external potentials. Through the Painlevé analysis some explicit requirements on the dispersion, nonlinearity, dissipation/gain, and the external potential as well as the constraint conditions are identified. It provides an explicit way to engineer integrable nonautonomous NLS equations at least in the sense of Painlevé integrability.

Osmoregulation in juvenile Chinese sturgeon (Acipenser sinensis Gray) during brackish water adaptation.

The osmoregulation capabilities of 7-month-old juvenile Chinese sturgeon (Acipenser sinensis Gray) (128.8 +/- 15 g) transferred directly from fresh water (0 per thousand, 46 mOsmol kg(-1)) to brackish water (10 per thousand, 273 mOsmol kg(-1)) were studied over a 20-day period. Changes in serum osmolarity, chloride (Cl(-)), sodium (Na+), potassium (K+) and calcium (Ca2+) ion concentrations, as well as gill and spiral valve Na+,K+-ATPase activities were measured at 3, 12, 24, 72, 216 and 480 h after transfer to BW.

[Reproductive biology of Macrobrachium nipponensis in Lake Wuhu].

Macrobrachium nipponensis is a key species of freshwater shrimps in China. To know its reproductive biology is very important for its protection and rational use. Studies from September 1998 to September 1999 in LakeWuhu showed that the spawning ground of M.