Analysis of the Roles of the Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells.

Zearalenone (ZEN) is one of the mycotoxins that pose high risks for human and animal health, as well as food safety. However, the regulators involved in ZEN cellular toxicity remain largely unknown. Herein, we showed that cell viability of porcine intestinal epithelial cells (IPEC-J2) tended to decrease with increasing doses of ZEN by the cell counting kit-8 assay.

Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Extracts.

(Ljub.) L.W.

[Correlation analysis of color change and Maillard reaction during processing of Gardeniae Fructus Praeparatus].

The chromaticity space parameters of the samples during the processing of Gardeniae Fructus Praeparatus(Jiaozhizi in Chinese herbal name, JZZ) were measured by the visual analyzer to analyze the color variation rule during the processing of JZZ, and the content changes of total reducing sugar, total amino acid and 5-hydroxymethylfurfural(5-HMF) related to Maillard reaction were measured. Pearson correlation analysis and linear regression analysis of the data were carried out by SPSS 24.0 software.

Treatment Mechanism of Fructus and Its Carbonized Product Against Ethanol-Induced Gastric Lesions in Rats.

Fructus (GF) and carbonized GF (GFC) have been shown to exert a gastrointestinal protective effect and are frequently used in clinical practice for the treatment of hemorrhage and brown stool. In this study, we employed a combination of pharmacological methods and metabolomics in a rat model of ethanol-induced acute stomach ulcer to investigate the gastroprotective effect of GF and GFC water extracts and the potential mechanism involved in this process. The levels of nitric oxide (NO) and interleukin 6 (IL-6) in the plasma of rats were determined.

The Potential Antipyretic Mechanism of Gardeniae Fructus and Its Heat-Processed Products With Plasma Metabolomics Using Rats With Yeast-Induced Fever.

Gardeniae Fructus (GF), prepared GF (GFP), and carbonized GF (GFC) are widely used in China for the treatment of fever. However, the involved antipyretic mechanism has not been fully elucidated. In this study, rectal temperature and pyrogenic cytokines were used to evaluate the antipyretic effect of raw and processed GF in rats with dry-yeast-induced pyrexia.

ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTS OF COMPOUND .

Background: Chinese medicine has its own uniqueness, advantageous in the treatment of hepatic diseases, and they were widely used in the oxidation. At the same time, oxidation is one of the mechanism of protect liver.

Materials And Methods: In the present study, the antioxidant activity of different extracts from Compound Lobelia were estimated respectively by the methods of measuring the [2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid] diamonium salt (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP).

[Analysis of volatile ingredients in Gardeniae Fructus and its processed products by GC-MS].

Gardeniae Fructus contains volatile ingredients, however, the species and proportions in different processed products of Gardeniae Fructus are different. In this experiment, volatile ingredients were separated by steam distillation with content of 1.2, 1.

[Research progress on processed Chinese medicine in vivo].

Processed Chinese medicine is the core of traditional Chinese medicine (TCM) industry chain,which directly affects the clinical efficacy and. safety of Chinese patent medicine and clinical formula Decoction pieces. Studied the variation of effective substance in vivo Chinese medicine processing before and after processed, clarifying the effective substance and processing principle is a top priority of the development of Chinese medicine processing.

Antioxidant activities in vitro and hepatoprotective effects of Nelumbo nucifera leaves in vivo.

Background: Herbal medicines played a major role in the treatment of hepatic disorders, and a number of medicinal plants and their compounds were widely used for the treatment of these disorders, and oxidant stress injury was one of the mechanism of liver injury.

Materials And Methods: Antioxidant activity of Nelumbo nucifera leaves (NU) extracts was assayed by the methods of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical and ferric reducing antioxidant power (FRAP) in vitro. By intraperitoneal injection carbon tetrachloride (CCl4) to establish acute liver injury model in mice, the levels of Glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), superoxide dismutase (SOD) and the content of and maleicdialdehyde (MDA) were detected to evaluate hepatoprotective effect of NU using corresponding test kit.

Antioxidant activity in vitro and hepatoprotective effect of Phlomis maximowiczii in vivo.

Background: A number of medicinal plants and there compounds played a major role in the treatment of hepatic disorders. They were widely used for the treatment of these disorders, and oxidant stress injury was one of the liver injury mechanisms. The present study evaluated the antioxidant activity and the hepatoprotective effect of each extracts of Phlomis maximowiczii.

[Content comparison of main chemical compositions in Gardenia jasminoids roasted with ginger juice].

Objective: To compare the contents of the main chemical compositions in Gardenia jasminoids before and after being roasted with ginger juice.

Method: Four diterpenoid pigments constituents (C-1, C-2, C-3, crocetin) were determined simultaneously by UPLC on an Agilent Poroshell 120 EC-C18 column at 35 degrees C with the methanol-0.5% formic acid anhydrous in gradient elution as the mobile phrase.

[New beta-carboline alkaloids contained in Psammosilene tunicoides].

Objective: To study the chemical constituents of Psammosilene tunicoides.

Method: The two chemical constituents were separated by various chromatographic methods, and their structures were identified on the basis of spectroscopic analysis.

Result: Two beta-carboline alkaloids were separated from normal butanol fraction of P.

[Analysis of volatile oil in Gardenia jasminoids roasted with ginger juice by GC-MS].

Objective: To compare the components and contents of volatile oil in Gardenia jasminoids, Zingiber officinale, G. jasminoids roasted with ginger juice and to provide scientific basis for intrinsic material of G. jasminoids roasted with ginger juice.

[Preparation of licorice juice as a processing excipient].

Objective: To develop the preparation method of licorice juice as a processing excipient and to determine the proper proportion of licorice juice and fructus evodiae during process.

Method: Nine experiments were carried out through L9 (3(4)) orthogonal design. The wetting results were compared by using different proportion of licorice juice and fructus evodiae.

[The HPLC fingerprint analysis of Fructus citri].

Objective: To study and establish the fingerprint of raw Fructus Citri by phased high performance liquid chromatography.

Method: The chromatographic conditions were as follows: a Kromasil C18 column was used; the mobile phase was composed of methanol and acetic-water(3.1:100, v/v) with gradient elution; the flow rate was 1.

[Effects of different types and standard of processing vinegaron inherent constituents in rhizoma of Corydalis yanhusuo].

Objective: To investigate the effects of processing adjuvants-different types of processing vinegar on effective constituents in rhizoma of Corydalis yanhusuo, and evaluate the quality of different types of vinegar in China.

Method: The HPLC method was adopted to determine the extraction solubility of dl-tetrahydropalmation and total alkaloids in rhizoma of Corydalis yanhusuo processed by vinegar. The sample extracts were separated on kromasil ODS column with mobile phase of methanol-1% phosphoric acid solution(65:35) and detection wavelength was 280 nm.

[Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC].

Objective: To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.

Method: Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.