Active heteromeric acetyl-CoA carboxylase forms polymorphic helical tubular filaments.

The heteromeric acetyl-CoA carboxylase (ACC) has four subunits assumed to form an elusive catalytic complex and are involved in allosteric and transcriptional regulation. The ACC represents almost all ACCs from pathogenic bacteria making it a key antibiotic development target to fight growing antibiotic resistance. Furthermore, it is a model for cyanobacterial and plant plastid ACCs as biofuel engineering targets.

The 2.6 Å Structure of a Tulane Virus Variant with Minor Mutations Leading to Receptor Change.

Human noroviruses (HuNoVs) are a major cause of acute gastroenteritis, contributing significantly to annual foodborne illness cases. However, studying these viruses has been challenging due to limitations in tissue culture techniques for over four decades. Tulane virus (TV) has emerged as a crucial surrogate for HuNoVs due to its close resemblance in amino acid composition and the availability of a robust cell culture system.

A comparative analysis of compression bearing capacity in recycled concrete brick walls and composite walls incorporating coal-ash blocks.

In the face of the problem of waste disposal in the demolition of concrete structures, a composite wall composed of recycled concrete bricks and fly ash blocks was proposed, and based on the previous thermal performance research, its axial compression performance were further studied. Four types of walls were designed and constructed: (1) clay brick masonry (CBM), (2) recycled concrete brick masonry (RBM), (3) bilateral clay bricks masonry with coal-ash blocks sandwich insulation wall (CFCM), and (4) bilateral recycled concrete bricks masonry with coal-ash blocks sandwich insulation wall (RFRM). The test results showed that recycled concrete brick masonry exhibited a higher bearing capacity than clay brick masonry.

"Is this blueberry ripe?": a blueberry ripeness detection algorithm for use on picking robots.

Blueberries are grown worldwide because of their high nutritional value; however, manual picking is difficult, and expert pickers are scarce. To meet the real needs of the market, picking robots that can identify the ripeness of blueberries are increasingly being used to replace manual operators. However, they struggle to accurately identify the ripeness of blueberries because of the heavy shading between the fruits and the small size of the fruit.

Discovery and Biological Characterization of PRMT5:MEP50 Protein-Protein Interaction Inhibitors.

Protein arginine methyltransferase 5 (PRMT5) is a master epigenetic regulator and an extensively validated therapeutic target in multiple cancers. Notably, PRMT5 is the only PRMT that requires an obligate cofactor, methylosome protein 50 (MEP50), to function. We developed compound , a novel small-molecule PRMT5:MEP50 protein-protein interaction (PPI) inhibitor, after initial virtual screen hit identification and analogue refinement.

Spatial and Temporal Variations of Polar Ionospheric Total Electron Content over Nearly Thirteen Years.

It is of great significance for the global navigation satellite system (GNSS) service to detect the polar ionospheric total electron content (TEC) and its variations, particularly under disturbed ionosphere conditions, including different phases of solar activity, the polar day and night alternation, the Weddell Sea anomaly (WSA) as well as geomagnetic storms. In this paper, four different models are utilized to map the ionospheric TEC over the Arctic and Antarctic for about one solar cycle: the polynomial (POLY) model, the generalized trigonometric series function (GTSF) model, the spherical harmonic (SH) model, and the spherical cap harmonic (SCH) model. Compared to other models, the SCH model has the best performance with ±0.

Study on the Quality Control for Periodogram in the Determination of Water Level Using the GNSS-IR Technique.

A GNSS station, located on the shore of sea and inland waters, and equipped with standard geodetic receivers and antennas, can be used to measure water levels using a technique called GNSS Interferometric Reflectometry (GNSS-IR). The classical GNSS-IR method is based on SNR data and LSP spectrum analysis method. In order to promote the application of GNSS-IR, the accuracy of the results needs to be further improved, and quality control needs to be achieved better.

Structural basis for reactivating the mutant TERT promoter by cooperative binding of p52 and ETS1.

Transcriptional factors ETS1/2 and p52 synergize downstream of non-canonical NF-κB signaling to drive reactivation of the -146C>T mutant TERT promoter in multiple cancer types, but the mechanism underlying this cooperativity remains unknown. Here we report the crystal structure of a ternary p52/ETS1/-146C>T TERT promoter complex. While p52 needs to associate with consensus κB sites on the DNA to function during non-canonical NF-κB signaling, we show that p52 can activate the -146C>T TERT promoter without binding DNA.

Structural and mechanistic insights into NDM-1 catalyzed hydrolysis of cephalosporins.

Cephalosporins constitute a large class of β-lactam antibiotics clinically used as antimicrobial drugs. New Dehli metallo-β-lactamase (NDM-1) poses a global threat to human health as it confers on bacterial pathogen resistance to almost all β-lactams, including penicillins, cephalosporins, and carbapenems. Here we report the first crystal structures of NDM-1 in complex with cefuroxime and cephalexin, as well as NMR spectra monitoring cefuroxime and cefixime hydrolysis catalyzed by NDM-1.

Structural basis for the unique heterodimeric assembly between cerebral cavernous malformation 3 and germinal center kinase III.

Defects in cerebral cavernous malformation protein CCM3 result in cerebral cavernous malformation (CCM), a common vascular lesion of the human CNS. CCM3 functions as an adaptor protein that interacts with various signal proteins. Among these partner proteins, germinal center kinase III (GCKIII) proteins have attracted significant interest because GCKIII-CCM3 interactions play essential roles in vascular physiology.

Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4.

MST4 is a member of the GCKIII kinases. The interaction between cerebral cavernous malformation 3 (CCM3) and GCKIII kinases plays a critical role in cardiovascular development and in cerebral cavernous malformations. The complex of CCM3 and the C-terminal domain of MST4 has been constructed, purified and crystallized, and a diffraction data set has been collected to 2.