Publications by authors named "Xuexue Cao"
In order to study the assembly mechanism of phycocyanin in red algae, the apo-phycocyanin genes (pcB and pcA) were cloned from Gracilariopsis lemaneiformis. The full length of phycocyanin β-subunit (pcB) contained 519 nucleotides encoding a protein of 172 amino acids, and the full length of phycocyanin α-subunit(pcA) contained 489 nucleotides encoding a protein of 162 amino acids. Expression vector pACYCDuet-pcB-pcA was constructed and transformed into E.
View Article and Find Full Text PDFArticle Synopsis
- Aurantiochytrium limacinum is recognized for its high levels of polyunsaturated fatty acids, specifically docosahexaenoic acid (DHA), which is produced via the polyketide synthase (PKS) pathway involving three gene clusters: pks1, pks2, and pks3.
- The researchers successfully amplified the full-length pks3 gene and identified key enzyme domains (KR and DH) that are important for DHA synthesis.
- By knocking in the KR and DH genes into A. limacinum, they increased the total fatty acid content and unsaturation levels, paving the way for enhanced industrial production of DHA.