Genetic Diagnosis and Clinical Features of Fetuses With Congenital Diaphragmatic Hernia.

Objective: Congenital diaphragmatic hernia (CDH) is a rare abnormality with highly heterogeneous genetic causes. This study investigated chromosomal and monogenic abnormalities in fetal CDH patients and evaluated the efficacy of chromosomal microarray analysis (CMA) and whole-exome sequencing (WES) for genetic diagnosis. The clinical features of the patients were also evaluated.

Layered Double Hydroxide (LDH)-Based Nanotripod for High-Entropydynamic Therapy Associated with Metabolism Homeostasis.

Multielemental transition metal compounds represent a class of promising candidates for the biomedical field due to their unique structure and biomedical application potential. However, their synthesis process remains challenging, which was subject to the high-temperature treatment of the multimetallic elements integrated within one system. Herein, for the first time, we have fabricated the nanotripod, , (FeCoNiCuZnAl)O (denoted as HEO) agent, via the structural topotactic transformation of layered double hydroxide (LDH) precursors with the tunable disorder degree, for highly efficient high-entropydynamic therapy associated with metabolism homeostasis.

Follow-up supportive policies and risk perception influence livelihood adaptation of anti-poverty relocated households in ethnic mountains of southwest China.

The poverty alleviation relocation program (PAR) is a milestone project in breaking spatial poverty traps in ethnic mountainous regions and provides a key solution for eradicating global absolute poverty. As direct participants in the restructuring of environmental and livelihood systems, the livelihood adaptation of relocated households is fundamental to the sustainable development of human-land systems in resettlement sites and the consolidation of the success of poverty alleviation. This research constructs an adaptive integration analysis framework of "external disturbance-adaptation process-adaptation outcome", by utilizing questionnaire data from relocated households in the Liangshan Yi minority area of Southwest China, and employing a formative structural equation model to investigate the transmission mechanisms and heterogeneous effects of PAR on livelihood adaptation across different resettlement characteristics.

Exploring exosomes: novel diagnostic and therapeutic frontiers in thyroid cancer.

In recent years, the incidence of thyroid cancer has surged globally, posing significant challenges in its diagnosis, treatment, and prognosis. Exosomes, as a class of extracellular vesicles, are secreted by nearly all cell types and encapsulate a variety of nucleic acids and proteins reflective of their cell of origin, thereby facilitating critical intercellular communication. Recent advancements in understanding these exosomes have catalyzed their application in oncology, particularly through uncovering their roles in the pathogenesis, diagnosis, and therapy of cancers.

Application value of strain elastography and shear wave elastography in patients with type 2 diabetic peripheral neuropathy: a prospective observational study.

Objective: To evaluate the value of conventional ultrasound (US), strain elastography (SE), and shear wave elastography (SWE) in detecting diabetic peripheral neuropathy (DPN) of the tibial nerve (TN), and to establish a predictive model for the diagnosis of DPN.

Methods: 32 healthy participants, 34 diabetic patients without DPN, and 36 diabetic patients with DPN were recruited for this study. The TN at the ankle and popliteal fossa were selected for examination.

Bacillus methanolicus: an emerging chassis for low-carbon biomanufacturing.

Bacillus methanolicus is a thermophilic methylotrophic bacterium that grows quickly on methanol in sea water-based media. It has been engineered for chemical bioproduction from methanol, but its efficiency needs improvement for industrialization. Synthetic biology approaches such as metabolic modeling and genome editing can reprogram B.

Compartment syndrome associations with drugs: a pharmacovigilance study of the FDA adverse event reporting system (FAERS).

Background: Compartment syndrome is an uncommon but life-threatening condition. No study has comprehensively compared compartment syndrome (CS) association with available drugs. The objective of this study was to estimate the association between CS and drugs using the FDA Adverse Event Report System (FAERS).

Triple-Responsive, Multimodal, Visual Electronic Skin toward All-in-One Health Management for Gestational Diabetes Mellitus.

Gestational diabetes mellitus (GDM) is one of the most common metabolic disorders during pregnancy, leading to serious complications for pregnant women and a threat to life safety of infants. Therefore, it is particularly important to establish a multipurpose monitoring pathway to important physiological indicators of pregnant women. In this work, three kinds of double network hydrogels are prepared with poly(vinyl alcohol) (PVA), borax, and cellulose ethers with varying substituents of methyl (methyl cellulose, MC), hydroxypropyl (hydroxypropyl cellulose, HPC), or both (hydroxypropyl methyl cellulose, HPMC), respectively.

Nitroaromatic compounds in six major Chinese cities: Influence of different formation mechanisms on light absorption properties.

Nitroaromatic compounds (NACs) are important nitrogen organics in aerosol with strong light-absorbing and chemically reactive properties. In this study, NACs in six Chinese megacities, including Harbin (HB), Beijing (BJ), Xi'an (XA), Wuhan (WH), Chengdu (CD), and Guangzhou (GZ), were investigated for understanding their sources, gas-particle partitioning, and impact on BrC absorption properties. The concentrations of ΣNACs in PM in the six cities ranged from 9.

Proteomic and metabolomic features in patients with HCC responding to lenvatinib and anti-PD1 therapy.

Combination therapy (lenvatinib/programmed death-1 inhibitor) is effective for treating unresectable hepatocellular carcinoma (uHCC). We reveal that responders have better overall and progression-free survival, as well as high tumor mutation burden and special somatic variants. We analyze the proteome and metabolome of 82 plasma samples from patients with hepatocellular carcinoma (HCC; n = 51) and normal controls (n = 15), revealing that individual differences outweigh treatment differences.

Insight into the Role of NH/NH and NO/NO in the Formation of Nitrogen-Containing Brown Carbon in Chinese Megacities.

Particulate brown carbon (BrC) plays a crucial role in the global radiative balance due to its ability to absorb light. However, the effect of molecular formation on the light absorption properties of BrC remains poorly understood. In this study, atmospheric BrC samples collected from six Chinese megacities in winter and summer were characterized through ultrahigh-performance liquid chromatography coupled with Orbitrap mass spectrometry (UHPLC-Orbitrap MS) and light absorption measurements.

Injectable Xenogeneic Dental Pulp Decellularized Extracellular Matrix Hydrogel Promotes Functional Dental Pulp Regeneration.

The present challenge in dental pulp tissue engineering scaffold materials lies in the development of tissue-specific scaffolds that are conducive to an optimal regenerative microenvironment and capable of accommodating intricate root canal systems. This study utilized porcine dental pulp to derive the decellularized extracellular matrix (dECM) via appropriate decellularization protocols. The resultant dECM was dissolved in an acid pepsin solution to form dECM hydrogels.

FAXDC2 inhibits the proliferation and invasion of human liver cancer HepG2 cells.

The reprogramming of lipid metabolism serves an important role in occurrence and development of liver cancer. Fatty acid hydroxylase domain containing 2 (FAXDC2) is a hydroxylase involved in the synthesis of cholesterol and sphingomyelin and downregulated in various types of cancer. There are no reports on the relationship between FAXDC2 and liver carcinogenesis.

Insights the dominant contribution of biomass burning to methanol-soluble PM bounded oxidation potential based on multilayer perceptron neural network analysis in Xi'an, China.

Atmospheric fine particulate matter (PM) is associated with cardiorespiratory morbidity and mortality due to its ability to generate reactive oxygen species (ROS). Ambient PM samples were collected during heating and nonheating seasons in Xi'an, China, and the ROS-generation potential of PM was quantified using the dithiothreitol (DTT) assay. Additionally, positive matrix factorization combined with multilayer perceptron was employed to apportion sources contributing to the oxidation potential of PM.

Panoramic variation analysis of a family with neurodevelopmental disorders caused by biallelic loss-of-function variants in TMEM141, DDHD2, and LHFPL5.

Highly clinical and genetic heterogeneity of neurodevelopmental disorders presents a major challenge in clinical genetics and medicine. Panoramic variation analysis is imperative to analyze the disease phenotypes resulting from multilocus genomic variation. Here, a Pakistani family with parental consanguinity was presented, characterized with severe intellectual disability (ID), spastic paraplegia, and deafness.

Discrepant diversity patterns and function of bacterial and fungal communities on an earthquake-prone mountain gradient in Northwest Sichuan, China.

Patterns of microbial diversity on elevational gradients have been extensively studied, but little is known about those patterns during the restoration of earthquake-fractured alpine ecosystems. In this study, soil properties, soil enzyme activities, abundance and diversity of soil bacterial and fungal communities at four positions along a 2.6-km elevational gradient in the Snow Treasure Summit National Nature Reserve, located in Pingwu County, Southwest China.

14-3-3 proteins regulate the HCHO stress response by interacting with AtMDH1 and AtGS1 in tobacco and Arabidopsis.

Formaldehyde (HCHO) is one of the most essential common carcinogenic environmental pollutants. While 14-3-3 proteins are known to regulate the response of plants to HCHO stress, the regulatory mechanisms responsible for a tolerant phenotype remain unclear. We first performed qPCR analysis of HCHO-treated Arabidopsis and tobacco and determined that the expression of At14-3-3PSI and Nt14-3-3C genes was rapidly upregulated after HCHO stress.

Cost-effectiveness analysis of ceftazidime-avibactam as definitive treatment for treatment of carbapenem-resistant bloodstream infection.

Background: Ceftazidime-avibactam (CAZ-AVI) is a novel antibiotic that has been confirmed in the United States and China for use in patients with carbapenem-resistant (CRKP) bloodstream infection (BSI). However, the cost-effectiveness of CAZ-AVI is unknown in China. This study aimed to evaluate the cost-effectiveness of CAZ-AVI compared to polymyxin B (PMB) monotherapy or PMB-based therapy for the treatment of CRKP BSI from the Chinese healthcare perspective.

Insights into the nitroaromatic compounds, formation, and light absorption contributing emissions from various geological maturity coals.

Nitroaromatic compounds (NACs) are essential components of atmospheric organic aerosols. Coal combustion is a key source of atmospheric NACs. In this study, a triple-quadrupole liquid chromatography-mass spectrometry (LC-MS) system was used to identify ten individual NAC emitted in combustions of chunk coal and its briquette at different maturity levels.

Layered double hydroxide-based nanozyme for NO-boost multi-enzyme dynamic therapy with tumor specificity.

The development of dual chemodynamic therapy and NO therapy can significantly improve the efficiency of cancer treatment. Therefore, designing a multifunctional agent to take full advantage of them and maximize their therapeutic effect remains a challenging goal. Herein, we have developed a novel LDHzyme by the confinement of L-arginine (L-Arg) on the surface of Mn-LDH nanosheets.

A 3D-Bioprinted Functional Module Based on Decellularized Extracellular Matrix Bioink for Periodontal Regeneration.

Poor fiber orientation and mismatched bone-ligament interface fusion have plagued the regeneration of periodontal defects by cell-based scaffolds. A 3D bioprinted biomimetic periodontal module is designed with high architectural integrity using a methacrylate gelatin/decellularized extracellular matrix (GelMA/dECM) cell-laden bioink. The module presents favorable mechanical properties and orientation guidance by high-precision topographical cues and provides a biochemical environment conducive to regulating encapsulated cell behavior.

Bionic Assembly of Layered Double Hydroxides Nanosheets and Positively Charged Micelles by Counterions Balance and Their Selective Detection of Mannose.

The selective detection of mannose is significant for tumor early diagnosis. However, current methods for detecting mannose are expensive and time-consuming, limiting their application. In this paper, we have obtained a 25-layer positively charged micellar/LDHs nanocomposite film system by electrostatic layer-by-layer assembly with reference to the unique properties of homogeneous charge ion attraction and charge overcompensation in biomolecules: hexadecyl trimethylammonium bromide (CTAB) was used to coat neutral molecules of fluorescein (FLU) to form (FLU@CTAB) cationic micelles, which were electrostatically assembled with laminate positively charged layered double hydroxides (LDHs) nanosheets to form (FLU@CTAB/LDHs) ultrathin films (UTFs) by the layer-by-layer electrostatic assembly, where the mediating role of the Br counteranion had a profound effect on the success of the assembly.