Integrated Transcriptomic-Metabolomic Analysis Reveals the Effect of Different Light Intensities on Ovarian Development in Chickens.

Light is a key environmental factor regulating reproduction in avians. However, the mechanism of light intensity regulating ovarian development is still unclear. In this study, 5-week-old (5 wk) partridge broiler breeders were randomly divided into a low-light-intensity group (LL group) and a natural-light-intensity group (NL group) ( = 100).

Dual-Temperature/pH-Sensitive Hydrogels with Excellent Strength and Toughness Crosslinked Using Three Crosslinking Methods.

Hydrogels are widely used as excellent drug carriers in the field of biomedicine. However, their application in medicine is limited by their poor mechanical properties and softness. To improve the mechanical properties of hydrogels, a novel triple-network amphiphilic hydrogel with three overlapping crosslinking methods using a one-pot free-radical polymerization was synthesized in this study.

Bilayered PLGA/Wool Keratin Composite Membranes Support Periodontal Regeneration in Beagle Dogs.

Regenerative procedures using guided tissue regeneration (GTR) membrane are presently well-established in periodontal therapy. In this study, bilayered poly(lactic--glycolic acid) (PLGA)/wool keratin (WK) composite membranes were prepared for use as GTR membrane by the solvent casting and electrospinning methods. Then, the composite membranes were used to evaluate the effects in guided tissue regeneration in beagle dogs.

Construction and characterization of osteogenic and vascular endothelial cell sheets from rat adipose-derived mesenchymal stem cells.

In this study, adipose-derived mesenchymal stem cells (ADSCs) were isolated from adipose tissues of rats. Flow cytometry identification showed that ADSCs of passage 3 highly expressed CD29 and CD44, but hardly expressed CD31 and CD45. Adipogenic, osteogenic, and chondrogenic differentiation were confirmed by the results of oil red O staining, alkaline phosphatase (ALP), and alcian blue staining, respectively.