A novel bi-functional DNA vaccine expressing VP1 protein and producing antisense RNA targeted to 5'UTR of foot-and-mouth disease virus can induce both rapid inhibitory effect and specific immune response in mice.

To overcome the inability of a conventional vaccine against a virus to induce rapid protection against viral challenge, a novel strategy was performed to generate a novel bi-functional vector expressing antisense RNA targeted to 5' untranslated regions (UTR) and VP1 protein of foot-and-mouth disease virus (FMDV). FMDV 5'UTR containing the viral RNA replication start elements was inserted inversely into the pIRES vector to produce antisense RNA, followed by insertion of FMDV VP1 gene to generate a recombinant plasmid pAS-IR-VP1. BHK-21 cells transfected with pAS-IR-VP1 plasmid showed a specific resistance against FMDV infection.