Background: Exosomes are released by most cell types, including tumor-associated macrophages (TAMs), transfer diverse macromolecules and participate in intercellular communication in cancer. However, whether M2-polarized TAMs (M2-TAMs)-derived exosomes (M2-exos) transmit the oncogenic protein malate dehydrogenase 1 (MDH1) to reprogram lung adenocarcinoma (LUAD) cancer cells is unknown.
Methods: THP-1-differentiated macrophages were co-cultured with A549 cells to generate TAMs (M0-TAMs and M2-TAMs).
Background: Endometrial cancer represents a significant health challenge, with rising incidence and complex prognostic challenges. This study aimed to develop a robust predictive model integrating programmed cell death-related genes and advanced machine learning techniques.
Methods: Utilizing transcriptomic data from TCGA-UCEC and GSE119041 datasets, we employed a comprehensive approach involving 117 machine learning algorithms.
Background: Mastitis caused by Staphylococcus aureus (S. aureus) is one of the most intractable problems for the dairy industry, causing significantly reduced milk yields and early slaughter of cows worldwide. MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and studies in recent years have shown the importance of miRNA-associated gene regulation in S.
View Article and Find Full Text PDFObjectives: To assess the efficacy of escitalopram combined with quetiapine vs quetiapine alone in the treatment of depressive episodes of bipolar disorder (BPD).
Methods: 88 hospitalized patients with a BPD depressive episode were recruited into the study. All patients were randomized to a control group (n = 44) or the intervention group (n = 44).
Braz J Otorhinolaryngol
January 2025
Objective: Pharyngocutaneous Fistula (PCF) is one of the most common and challenging postoperative complications after Total Laryngectomy (TLE). This study aimed to evaluate the impact of the modified Vertical Pressure Bandage (VPB) on the occurrence of Pharyngocutaneous Fistula (PCF) after Total Laryngectomy (TLE) for Head and Neck (H&N) cancer.
Methods: This retrospective study included patients with H&N cancer who underwent TLE at our hospital between January 2010 and January 2021.
Yeast shows promise as a delivery system for drugs and vaccines due to its specific targeting and immunogenic properties. The objective of this research is to create novel and effective yeast-based methods for delivering subunit vaccines. Through the modification of yeast expression plasmids and optimization of expression techniques, a new dual-expression system has been developed.
View Article and Find Full Text PDFThis study aims to explore the feasibility of applying the "Three-Low" technique (low injection rate, low iodine contrast volume, low radiation dose) in coronary CT angiography (CCTA). We prospectively collected data from 90 patients who underwent CCTA at our hospital between 2021 and 2024. The patients were randomly assigned to either the experimental group (n = 45) or the control group (n = 45).
View Article and Find Full Text PDFBackground: Intrahepatic cholangiocarcinoma is a malignant tumor that starts from the epithelium of the bile duct and has a poor prognosis. They are characterized by poor response to chemotherapy and lack of effective targeted therapies; thus, therapeutic options are limited.
Case Presentation: A 59-year-old man was admitted to the hospital for a workup of abnormal CA19-9 levels.
Rabies is an acute lethal disease causing by the neurotropic virus rabies virus (RABV). Rabies immune globulin (RIG) as an indispensable component of rabies postexposure prophylaxis (PEP) always faces with great challenges in terms of costs, stability and safety. Our objective is to develop a novel and potential fully human monoclonal antibodies (mAbs) cocktail for the improvement of rabies PEP.
View Article and Find Full Text PDFThe rearing system of livestock plays a vital role in animal production, meat quality, and overall welfare. This study aimed to assess the influence of cage-rearing system and forest-rearing system on the ileum microbiota, metabolome, and ileal mucosa transcriptome in meat rabbits. Moreover, 16S rDNA sequencing revealed significant differences in the ileal microbiome composition: caged rabbits exhibited a higher abundance of the genera uncultured and , whereas the levels of , unclassified , and uncultured were lower compared to rabbits reared in the forest.
View Article and Find Full Text PDFMetabolic reprogramming is a common feature in tumor progression and metastasis. Like proteins, lipids can transduce signals through lipid-protein interactions. During tumor initiation and metastasis, dysregulation of the Hippo pathway plays a critical role.
View Article and Find Full Text PDFEmerg Microbes Infect
December 2024
Background: Spring viremia of carp virus (SVCV) infects a wide range of fish species and causes high mortality rates in aquaculture. This viral infection is characterized by seasonal outbreaks that are temperature-dependent. However, the specific mechanism behind temperature-dependent SVCV infectivity and pathogenicity remains unclear.
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