Total Synthesis of (-)- and (+)-Talaroenamine B and Diphenylene Derivatives.

The first total synthesis of (±)-talaroenamine B was achieved through a concise four-step procedure. The key feature of this synthetic strategy lies in a one-pot reaction involving I (III)-mediated oxidative dearomatization to construct a racemic cyclohexanedione unit, followed by imination using a chiral auxiliary to afford a separable mixture of diastereoisomers. A further acid-catalyzed substitution reaction of aniline with the diastereoisomers led to the natural product (-)-talaroenamine B and its enantiomer (+)-talaroenamine B.

Soybean-mediated suppression of BjaI/BjaR quorum sensing in impacts symbiotic nitrogen fixation.

The acyl-homoserine lactones (AHLs)-mediated LuxI/LuxR quorum sensing (QS) system orchestrates diverse bacterial behaviors in response to changes in population density. The role of the BjaI/BjaR QS system in USDA 110, which shares homology with LuxI/LuxR, remains elusive during symbiotic interaction with soybean. Here this genetic system in wild-type (WT) bacteria residing inside nodules exhibited significantly reduced activity compared to free-living cells, potentially attributed to soybean-mediated suppression.

Telephone instructions improve the quality of bowel preparation for colonoscopy: A meta-analysis of randomized controlled trials.

Objective: To evaluate the effect of telephone instructions on the quality of bowel preparation in patients undergoing colonoscopy.

Methods: Online English databases (PubMed, Web of Science, Cochrane Library, and Embase) were screened for randomized controlled trials on telephone instructions regarding bowel preparation for colonoscopy from inception to April 15, 2022. After data extraction, the Review Manager software was used for meta-analysis.

Aniline-induced production of aniline-containing polyketides and related bicyclic polyketides by the Yellow River wetland-derived fungus .

Introduction And Methods: Silencing gene activation can effectively enrich the diversity of fungal secondary metabolites.

Results And Discussion: Cultivation of the Yellow River wetland-derived fungus HPU-Y01 with aniline led to the isolation of one new aniline-containing polyketide tanicutone A (), two new bicyclic polyketides tanicutones B-C (-), a new related trienoic acid 8-methyldeca-2,4,6-trienoic acid (), and a known compound . The planar structures and configurations of - were determined by NMR, MS, and ECD calculations.

Stress response and influencing factors among fever outpatients and children's families during normalisation of the COVID-19 epidemic: A cross-sectional study.

Aim: To examine the demographic characteristics associated with stress response of fever outpatients and children's families during normalisation of the COVID-19 epidemic and to examine the relationship between stress response, coping style and resilience.

Design: Online cross-sectional study.

Methods: A total of 541 fever clinic participants from Yiwu, China, were recruited via WeChat from February to November 2021.

Production of Multiple Talaroenamines from via One-Pot/Two-Stage Precursor-Directed Biosynthesis.

Nineteen new talaroenamine derivatives, talaroenamines F1-F19 (-), were isolated from the Yellow River wetland derived HPU-J01 by use of a one-pot/two-stage precursor-directed biosynthesis approach. During this approach, the initial precursor -methylaniline was first used as a carrier to capture the biologically synthesized cyclohexanedione to produce talaroenamine F, and then the other aniline derivatives were employed to replace the -methylaniline fragment of talaroenamine F to generate the final products. LC-MS analysis showed that only four compounds (, , , and ) could be produced by the traditional precursor-directed biosynthesis in which the aniline precursors were added simultaneously.

Patient education by smartphones for bowel preparation before colonoscopy.

Background And Aim: We aim to evaluate the effect of smartphone education on the bowel preparation quality of patients undergoing colonoscopy by meta-analysis.

Methods: Randomized controlled trials using smartphones to educate patients on bowel preparation for colonoscopy were screened from the PubMed, Web of Science, Cochrane Library, and Embase databases from inception to August 31, 2021. After extracting the data, Review Manager software was used for meta-analysis.

Impact of Patient Education via WeChat on Bowel Preparation for Colonoscopy: A Meta-analysis of Randomized Controlled Trials.

The aim of this study was to evaluate the impact of education via WeChat on the quality of bowel preparation in patients undergoing colonoscopy through a meta-analysis of randomized controlled trials. Randomized controlled trials of bowel preparation education provided via WeChat for patients undergoing colonoscopy were screened from databases such as PubMed, Web of Science, Cochrane Library, and Embase. Papers published from the date of database construction to May 1, 2021, were extracted, and a meta-analysis was performed using Review Manager software.

Reinforced education by short message service improves the quality of bowel preparation for colonoscopy.

Purpose: This study aimed to evaluate the effect of reinforced education (RE) by short message service (SMS) on the bowel preparation quality of patients undergoing colonoscopy.

Methods: Randomized controlled trials (RCTs), conducted on the effect of RE by SMS on bowel preparation for colonoscopy from inception to November 1, 2021, were queried from databases, including PubMed, Web of Science, the Cochrane Library, and EMBASE. After extracting the data, meta-analysis was conducted using Review Manager Software version 5.

Precursor-Directed Biosynthesis of Talaroenamine Derivatives Using a Yellow River Wetland-Derived .

Precursor-directed biosynthesis was used to introduce selected aniline derivatives into the talaroenamine pathway, which had recently been defined from a Yellow River wetland-derived HPU-J01. The known talaroenamine B () and six previously undescribed talaroenamine derivatives, talaroenamines F-K (-), were generated and structurally characterized. The aniline derivatives are introduced via nonenzymatic addition to the reactive intermediate cyclohexanedione.

Involvement of a Novel TetR-Like Regulator (BdtR) of in the Efflux of Isoflavonoid Genistein.

A wide variety of leguminous plant-released (iso)flavonoids, such as genistein, are potential inducers of the nodulation () genes of endosymbiotic rhizobia for the production of Nod factors, which are vital signaling molecules for triggering the symbiotic process. However, these (iso)flavonoids are generally thought to be toxic to the bacterial partner to varying degrees. Here, a novel TetR-like regulator gene of the soybean symbiont USDA110, (systematic designation ), was characterized.

A meta-analysis of randomized controlled trials of the addition of lubiprostone to bowel preparation before colonoscopy.

Aim: Adequate bowel preparation is essential to the quality of colonoscopy. We performed a meta-analysis to determine the efficacy and safety of the addition of lubiprostone to the bowel preparation process prior to colonoscopy.

Methods: Online databases, namely, PubMed, MEDLINE and Cochrane Library, were searched for randomized controlled trials that assessed the additive effect of lubiprostone on the quality of colon preparation in patients undergoing colonoscopy.

Correction to: Varilactones and wortmannilactones produced by Penicillium variabile cultured with histone deacetylase inhibitor.

The author would like to include conflict of interest statement of the online published article. The correct conflict of interest statement should read as.

Sorbicillasins A⁻B and Scirpyrone K from a Deep-Sea-Derived Fungus, sp. FL30r.

Two new nitrogen-containing sorbicillinoids named sorbicillasins A and B ( and ) and a new 3,4,6-trisubstituted -pyrone derivative, scirpyrone K (), together with two known biosynthetically related polyketides (⁻), were isolated from the deep-sea-derived fungus sp. FL30r by using the OSMAC (one strain-many compounds) method. The structures of ⁻, including absolute configurations, were deduced based on MS, NMR, and time-dependent density functional theory (TD-DFT) calculations of specific ECD (electronic circular dichroism) spectra.

Aniline-Tetramic Acids from the Deep-Sea-Derived Fungus Cladosporium sphaerospermum L3P3 Cultured with the HDAC Inhibitor SAHA.

Four new tetramic acids, cladosins H-K (1-4), and a related known compound, cladodionen (5), were isolated from the culture of the Mariana Trench (depth 6562 m) sediment-derived fungus Cladosporium sphaerospermum L3P3 treated with the histone deacetylase inhibitor SAHA (suberanilohydroxamic acid). Interestingly, compounds 1-5 existed as equilibrium E/ Z mixtures and 1-4 were the first cases of tetramic acids containing aniline moieties. Their structures including absolute configurations were elucidated through a combination of NMR, MS, and Mosher's method, together with the consideration of biogenetic origins.

Inducing Secondary Metabolite Production by Combined Culture of Talaromyces aculeatus and Penicillium variabile.

Four new polyketides, penitalarins A-C (1-3) and nafuredin B (4), together with the known biogenetically related nafuredin A (5) were isolated from a mixed culture of a deep-sea-derived fungus Talaromyces aculeatus and a mangrove-derived fungus Penicillium variabile. Liquid chromatography/mass spectrometry analysis showed that none of compounds 1-5 was produced by either of the two fungi when cultured alone under the same condition. The structures of 1-4, including absolute configurations, were deduced based on the interpretation of MS, NMR data, and time-dependent density functional theory calculations of specific electronic circular dichroism spectra.

Varilactones and wortmannilactones produced by Penicillium variabile cultured with histone deacetylase inhibitor.

Seven polyketides, including four new ones named as varilactones A-B (1-2) and wortmannilactones M-N (3-4), as well as three biogenetically related known wortmannilactones E, F and H (5-7), were isolated from the fungus Penicillium variabile HXQ-H-1, cultivated in potato-based medium with the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA). Their structures (1-7) were established by analysis of NMR and MS data, and the absolute configuration of oxabicyclo[2.2.

Late-Stage Terpene Cyclization by an Integral Membrane Cyclase in the Biosynthesis of Isoprenoid Epoxycyclohexenone Natural Products.

Macrophorins are representative examples of isoprenoid epoxycyclohexenones containing cyclized drimane moieties. We located and characterized the biosynthetic gene cluster of macrophorin from Penicillium terrestris. MacJ encoded by this cluster was characterized to be the first example of a membrane-bound type-II terpene cyclase catalyzing the cyclization of meroterpenoids via direct protonation of the terminal olefinic bond in acyclic yanuthones.

Peniphenylanes A-G from the Deep-Sea-Derived Fungus Penicillium fellutanum HDN14-323.

Seven new 6-methylsaligenin derivatives, including the trimeric peniphenylanes A-B (1-2) and dimeric peniphenylanes C-G (3-7), together with four known biogenetically related compounds (8-11) were discovered from the extract of the deep-sea-derived fungus Penicillium fellutanum HDN14-323. The structures of the new compounds were established through extensive analysis. Their cytotoxic activity against HeLa, HL-60, and HCT-116 cell lines was evaluated, with compound 4 exhibiting the best activity against the HeLa cell line (IC50 = 9.

Varitatin A, a Highly Modified Fatty Acid Amide from Penicillium variabile Cultured with a DNA Methyltransferase Inhibitor.

A new, highly modified fatty acid amide, varitatin A (1), was isolated from the fungus Penicillium variabile HXQ-H-1 cultivated with the DNA methyltransferase inhibitor 5-azacytidine. The structure including the absolute configuration of 1 was established by analysis of NMR and MS data, together with chemical degradation and Mosher's method based on MPA esters. Compound 1 showed cytotoxicity against HCT-116 cells with an IC50 value of 2.