[Effects of glucocorticoid receptor agonists on hyperalgesia of rats with neuropathic pain and its mechanisms].

Objective: To investigate the effects of glucocorticoid receptor agonists on hyperalgesia in rats with neuropathic pain (NPP) by regulating nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)/interleukin-1β (IL-1β) pathway and its mechanisms.

Methods: Forty SD rats were divided into control group, NPP model group, NPP treated with NLRP3 inhibitor group and dexamethasone treatment group with 10 rats in each group. The NPP rat model was induced by vincristine.

Prediction Value of Epilepsy Secondary to Inferior Cavity Hemorrhage Based on Scalp EEG Wave Pattern in Deep Learning.

Objective: To search the predictive value of epilepsy secondary to acute subarachnoid hemorrhage (aSAH) based on EEG wave pattern in deep learning.

Methods: A total of 156 cases of secondary epilepsy with lower cavity hemorrhage in our hospital were selected and divided into the late epilepsy group and the early epilepsy group according to seizure time, and the nonseizure group and the seizure group according to seizure condition. General data of patients were collected, the EEG types of each group were analyzed, and the disease recurrence rate, treatment effect, and symptom onset time were compared.

Deep Learning-Based Detection and Diagnosis of Subarachnoid Hemorrhage.

Subarachnoid hemorrhage (SAH) is one of the critical and severe neurological diseases with high morbidity and mortality. Head computed tomography (CT) is among the preferred methods for the diagnosis of SAH, which is confirmed by CT showing high-density shadow in the subarachnoid space. Analysis of these images through a deep learning-based subarachnoid hemorrhage will reduce the approximate rate of misdiagnosis in general and missed diagnosis by clinicians in particular.

Estrogen improved the regeneration of axons after subcortical axon injury via regulation of PI3K/Akt/CDK5/Tau pathway.

Aim: To investigate the effect of estrogen on axon regeneration and neurological recovery after subcortical axon injury, and further explore its underlying molecular mechanisms.

Method: Subcortical axonal fiber injury model was used in this study. Morris water maze was conducted to detect the learning and memory ability of the rats; modified neurological severity score (mNSS) and beam walking test were performed to evaluate the behavioral; and diffusion tensor imaging (DTI) was used for the determination of recovery after subcortical axonal injury, while Western blotting was performed to detect the expression of p-Akt, CDK5, p-Ser262, p-Ser404, and p-Thr205.

The relationship between estrogen-induced phenotypic transformation and proliferation of vascular smooth muscle and hypertensive intracerebral hemorrhage.

Background: To explore the effect of estrogen on human cerebral vascular smooth muscle cells (VSMCs) and to clarify the molecular mechanism of estrogen inhibition of VSMC proliferation, which could provide an important reference basis for the clinical treatment of hypertensive intracerebral hemorrhage.

Method: Firstly, the effects of different concentrations of estradiol and estrogen receptor (ESR) blocker (tamoxifen) on the proliferation of human VSMCs and the expression of estrogen-related receptor gene (ESR: ESR1, ESR2, GPER), myocardin (MYOCD), serum reaction factor (SRF), and apoptosis gene caspase-3 were measured to discover the effect and mechanism of tamoxifen on the proliferation and apoptosis of VSMCs. Secondly, the effects of estradiol on human VSMCs treated with angiotensin II (Ang II) were observed by measuring the expression of vascular smooth muscle markers, α-smooth muscle actin (α-SMA), SM22α, FLN, MCP-1, and TLR4.

The miR-455-5p/ERα36 axis regulates mammalian neuronal viability and axonal regeneration.

Estrogen signal transduction plays an important role in regulating neuronal cell growth and axonal regeneration. Estrogen receptor alpha 36 (ERα36) is a truncated isoform of the estrogen receptor that inhibits aspects of estrogen transduction. Here, we investigated the role of ERα36 in neuronal viability as well as axonal growth and regeneration using SH-SY5Y neuroblastoma cells and murine sensory neurons.

Sinomenine enhances microglia M2 polarization and attenuates inflammatory injury in intracerebral hemorrhage.

Microglia polarization plays a vital role in brain inflammatory injury following intracerebral hemorrhage (ICH). Previous studies have shown that sinomenine possesses potential immunoregulatory capabilities. However, microglia polarization's exact mechanisms in ICH remain uncertain.

MCP-1-mediated activation of microglia promotes white matter lesions and cognitive deficits by chronic cerebral hypoperfusion in mice.

Microglia activation played a vital role in the pathogenesis of white matter lesions (WMLs) by chronic cerebral hypoperfusion. In addition, hypoxia induced up-regulated expression of MCP-1, promotes the activation of microglia. However, the role of MCP-1-mediated microglia activation in chronic cerebral ischemia is still unknown.

The Role of Platelet-Derived Growth Factor Receptor in Early Brain Injury Following Subarachnoid Hemorrhage.

Objective: This study aims to observe encephaledema and cell apoptosis in rats following subarachnoid hemorrhage (SAH) and to explore the mechanism of platelet-derived growth factor receptor (PDGFR) in the development of early brain injury (EBI).

Methods: Adult and male Sprague-Dawley rats were randomly divided into 4 groups: sham operation, SAH, SAH + imatinib, and SAH + platelet-derived growth factor-BB (PDGF-BB). SAH model was established using intravascular silk puncture of the internal carotid artery crotch.