Surface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms.

Biofilms are ubiquitous surface-associated bacterial communities embedded in an extracellular matrix. It is commonly assumed that biofilm cells are glued together by the matrix; however, how the specific biochemistry of matrix components affects the cell-matrix interactions and how these interactions vary during biofilm growth remain unclear. Here, we investigate cell-matrix interactions in Vibrio cholerae, the causative agent of cholera.

Incorporation of collagen into and biofilms impedes phagocytosis by neutrophils.

Biofilms are communities of microbes embedded in a matrix of extracellular polymeric substances (EPS). Matrix components can be produced by biofilm organisms and can also originate from the environment and then be incorporated into the biofilm. For example, we have recently shown that collagen, a host-produced protein that is abundant in many different infection sites, can be taken up into the biofilm matrix, altering biofilm mechanics.

Kindlin-2 promotes rear focal adhesion disassembly and directional persistence during cell migration.

Cell migration involves front-to-rear asymmetric focal adhesion (FA) dynamics, which facilitates trailing edge detachment and directional persistence. Here, we show that kindlin-2 is crucial for FA sliding and disassembly in migrating cells. Loss of kindlin-2 markedly reduced FA number and selectively impaired rear FA sliding and disassembly, resulting in defective rear retraction and reduced directional persistence during cell migration.

Pure Acetylene Semihydrogenation over Ni-Cu Bimetallic Catalysts: Effect of the Cu/Ni Ratio on Catalytic Performance.

Ethylene is an important chemical raw material and with the increasing consumption of petroleum resources, the production of ethylene through the calcium carbide acetylene route has important research significance. In this work, a series of bimetallic catalysts with different Cu/Ni molar ratios are prepared by co-impregnation method for the hydrogenation of calcium carbide acetylene to ethylene. The introduction of an appropriate amount of Cu effectively inhibits not only the formation of ethane and green oil, thus increasing the selectivity of ethylene, but also the formation of carbon deposits, which improves the stability of the catalyst.

[Activation of platelet activating factor receptor by Acinetobacter baumannii results in oxidative stress and apoptosis in human bronchial epithelial cells].

Objective To investigate the effect of platelet activating factor receptor (PAFR) on human bronchial epithelial (HBE) cells infected by Acinetobacter baumannii (A. baumannii). Methods HBE cells were divided into control group, ginkgolide B (GB) group, A.

[Blockade of TLR4/NF-κB pathway promotes the inflammation of Acinetobacter baumannii-infected rats].

Objective To explore the effects of TLR4 on Acinetobacter baumannii (A. baumannii) infection in a rat model. Methods Healthy male SD rats were divided into normal control group, TAK-242 treated group, A.

[Caspase-1 inhibitor AC-YVAD-CMK blocks IL-1β secretion of bone marrow-derived macrophages induced by Acinetobacter baumannii].

Objective To explore the effect of caspase-1 selective inhibitor AC-YVAD-CMK on IL-1β secretion of bone marrow-derived macrophages (BMDMs) induced by Acinetobacter baumannii (A. baumannii). Methods Macrophages were separated from C57BL/6 mice which were stimulated using different concentrations of A.

[Establishment of Acinetobacter baumannii-induced pneumonia model in mice].

Objective To establish Acinetobacter baumannii (A. baumannii)-induced pneumonia models in C57BL/6 mice, and study the molecule mechanism of A. baumannii infection.

Assessment of heavy metals contamination in sediments from three adjacent regions of the Yellow River using metal chemical fractions and multivariate analysis techniques.

Metal chemical fractions obtained by optimized BCR three-stage extraction procedure and multivariate analysis techniques were exploited for assessing 7 heavy metals (Cr, Pb, Cd, Co, Cu, Zn and Ni) in sediments from Gansu province, Ningxia and Inner Mongolia Autonomous Regions of the Yellow River in Northern China. The results indicated that higher susceptibility and bioavailability of Cr and Cd with a strong anthropogenic source were due to their higher availability in the exchangeable fraction. A portion of Pb, Cd, Co, Zn, and Ni in reducible fraction may be due to the fact that they can form stable complexes with Fe and Mn oxides.