Atroposelective Construction of C-B Axial Chirality via N-Heterocyclic Carbene-Catalyzed Dynamic Kinetic Resolution.

C-B axially chiral architectures are valuable in materials science and medicinal chemistry, but their enantioselective synthesis remains a challenge. Herein, we report an efficient method for the enantioselective synthesis of C-B axially chiral 1,2-azaborines through N-heterocyclic carbene-catalyzed dynamic kinetic resolution. Treatment of racemic 1,2-azaborine-based arylaldehyde with a chiral N-heterocyclic carbene catalyst under oxidative conditions in the presence of an alcohol leads to atroposelective esterification with up to 97% yield and 98% ee.

How physical exercise enhances life satisfaction in Chinese senior college students: mediating roles of self-efficacy and resilience.

Objective: This study explored the relationship between physical exercise and life satisfaction among senior college students by focusing on the mediating roles of self-efficacy and resilience, thereby providing theoretical support for the mental health benefits of physical exercise for senior students.

Methods: A survey was conducted with 600 senior students from 10 universities using the physical exercise rating, life satisfaction, self-efficacy, and resilience scales. Data analysis was performed using SPSS 23.

Enantioselective Acylation of Benzylic C(sp3)-H Bond Enabled by a Cooperative Photoredox and N-Heterocyclic Carbene Catalysis.

Organocatalyzed direct and asymmetric functionalization of benzylic C(sp3)-H bond is attractive yet challenging. Herein, we report the enantioselective acylation of benzylic C(sp3)-H bond via a cooperative photoredox and N-heterocyclic carbene (NHC) catalysis, affording the corresponding chiral α-aryl ketones in moderate to good yields with good to excellent enantioselectivities (up to 99:1 er). The rational design of novel NHCs guided by initial evaluation of available catalysts and their application promote the asymmetric transformation.

BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cells.

Protein liquid-liquid phase separation (LLPS), a pivotal phenomenon intricately linked to cellular processes, is regulated by various other proteins. However, there is still a lack of high-throughput methods for screening protein regulators of LLPS in target proteins. Here, we developed a CRISPR/Cas9-based screening method to identify protein phase separation regulators by integrating bimolecular fluorescence complementation (BiFC) and fluorescence-activated cell sorting (FACS).

E3 ligase SYVN1-mediated polyubiquitination of CPSF6 promotes alternative polyadenylation and antivirus effects of macrophages.

Transcriptome-wide alternative polyadenylation (APA) is involved in both innate and adaptive immune responses of immune cells. Downregulation of the CPSF6 protein, one of the 3' end-processing factors, mediates APA in macrophages with responses to virus infection and plays an important role in its anti-virus effect. However, the signaling pathway and molecular mechanism underlying the downregulation of the CPSF6 protein remain elusive.

CMCS-PVA@CA hydrogel dressing: A promoter of wound healing with MRSA virulence attenuation function.

Traditional wound dressings, primarily centered on antimicrobial or bactericidal strategies, have inadvertently contributed to the rise of drug-resistant bacterial colonies at wound sites, thus prolonging the healing process. In this study, we developed an innovative hydrogel dressing, CMCS-PVA@CA, incorporating carboxymethyl chitosan (CMCS), polyvinyl alcohol (PVA), and cichoric acid (CA), specifically designed to treat skin wounds infected with methicillin-resistant Staphylococcus aureus (MRSA). Computational biology analyses reveal that CA exerts substantial anti-virulence activity by targeting serine/threonine phosphatase (Stp1), achieving an IC of 3.

Energy dependence of synchronization mode transitions in the delay-coupled FitzHugh-Nagumo system driven by chaotic activity.

Energy absorption and consumption are essential for the activity of single neurons and neuronal networks. The synchronization mode transition and energy dependence in a delay-coupled FitzHugh-Nagumo (FHN) neuronal system driven by chaotic activity are investigated in this paper. With the change of chaotic current intensity, it was found that the synchronization mode of coupled neurons undergoes synchronous state, transition state, anti-phase state, alternating asynchronous and anti-phase state, and chaotic current-induced chaotic state.

Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats.

Objective: The aim of this study was to obtain goat CRTC2 gene sequence and elucidate its biological properties, and further study the impact of overexpression and interference of CRTC2 on the cell differentiation of goat subcutaneous precursor adipocytes.

Methods: The sequence of goat CRTC2 was cloned by reverse transcription polymerase chain reaction (RT-PCR) and its molecular characterization was analyzed. The expression of CRTC2 gene in goat tissues and subcutaneous precursor adipocytes differentiated from 0 to 120 h was examined by quantitative real-time PCR (qRT-PCR).

Enantioselective Synthesis of 3-Hydroxy-2-Oxindoles via Ni-Catalyzed Asymmetric Addition of Aromatic Bromides to α-Ketoamides.

Nickel-catalyzed asymmetric intramolecular addition of aryl halides to α-ketoamides has been achieved to afford chiral 3-substituted-3-hydroxy-2-oxindoles in excellent yields and high enantioselectivities (up to 99 % yield and 98 % ee), which provides efficient access to valuable molecules containing 3-hydroxy-2-oxindole core. The gram-scale reaction proved the potential utility of the methodology.

Molecular Characterizations of and Its Functional Role in Inhibiting the Differentiation of Goat Intramuscular Adipocytes through RIG-I Receptor Signaling Pathway.

The aim of this study was to elucidate the effect of on the differentiation of goat intramuscular precursor adipocytes and its mechanism of action. Here, we cloned the CDS region 2094 bp of the goat gene, encoding a total of 697 amino acid residues. Functionally, overexpression of inhibited the differentiation of goat intramuscular adipocytes with a concomitant reduction in lipid droplets, whereas interference with expression promoted the differentiation of goat intramuscular adipocytes.

Efficient delivery of curcumin by functional solid lipid nanoparticles with promoting endosomal escape and liver targeting properties.

In the realm of intracellular drug delivery, overcoming the barrier of endosomal entrapment stands as a critical factor influencing the effectiveness of nanodrug delivery systems. This study focuses on the synthesis of an acid-sensitive fatty acid derivative called imidazole-stearic acid (IM-SA). Leveraging the proton sponge effect attributed to imidazole groups, IM-SA was anticipated to play a pivotal role in facilitating endosomal escape.

Pharmacokinetics and Safety of Linezolid Tablets of 2 Different Manufacturers in Healthy Chinese Subjects in Fasting and Fed States.

This study aimed to evaluate the pharmacokinetics (PKs) and safety of a generic drug, linezolid, compared to those of a reference drug in healthy Chinese subjects under both fasting and fed conditions. This was a randomized, open-label, 2-period, 2-sequence crossover study. The subjects received a single dose of the test or reference drug, linezolid (600 mg), in each period.

A novel memristive neuron model and its energy characteristics.

The functional neurons are basic building blocks of the nervous system and are responsible for transmitting information between different parts of the body. However, it is less known about the interaction between the neuron and the field. In this work, we propose a novel functional neuron by introducing a flux-controlled memristor into the FitzHugh-Nagumo neuron model, and the field effect is estimated by the memristor.

Prenatal Exposure of PFAS in Cohorts of Pregnant Women: Identifying the Critical Windows of Vulnerability and Health Implications.

Cohorts of pregnant women in 2018 and 2020 were selected to explore prenatal exposure to perfluoroalkyl and polyfluoroalkyl substances (PFAS). Maternal serum during the whole pregnancy (first to third trimesters) and matched cord serum were collected for the analysis of 50 PFAS. Perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), and 6:2 fluorotelomer sulfonic acid (6:2 FTS) were the dominant PFAS in both the maternal and cord serum.

How do life course events affect the accumulation of digital literacy?: Based on qualitative research of 16 medical university teachers of China.

The purpose of this article is to examine the level of the accumulation of digital literacy in medical university of China. In this study, we argue that positive life events facilitate the formation of digital literacy. We review the development of research of life course and digital literacy since the 2010.

High Levels of BPA and BPF Exposure during Pregnancy Are Associated with Lower Birth Weight in Shenyang in Northeast China.

Animal studies indicate that bisphenol A (BPA) has obesogenic effects. Recent experiments reported similar endocrine-disrupting effects of bisphenol F (BPF) and bisphenol S (BPS), which are substitutes of BPA. The aim of this study was to investigate the exposure levels of these bisphenols in pregnant women and their effects on the physical development of infants aged 0-12 months.

P2X3 Receptor Antagonist Eliapixant in Phase I Clinical Trials: Safety and Inter-ethnic Comparison of Pharmacokinetics in Healthy Chinese and Japanese Participants.

Background: Afferent neuronal hypersensitization via P2X3 receptor signaling has been implicated as a driver of several disorders, including refractory chronic cough, endometriosis, diabetic neuropathic pain, and overactive bladder. Eliapixant, a selective P2X3 receptor antagonist, has been in clinical development for all four disorders.

Objective: This paper describes pharmacokinetic (PK) and safety data from two phase I studies of eliapixant in healthy Japanese and Chinese participants and compares those data within the two populations and with previous multiple dose data from Caucasian participants.

RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31.

Colon adenocarcinoma (COAD) is the second leading cause of cancer death, and there is still a lack of diagnostic biomarkers and therapeutic targets. In this study, bioinformatics analysis of the TCGA database was used to obtain RUNX1, a gene with prognostic value in COAD. RUNX1 plays an important role in many malignancies, and its molecular regulatory mechanisms in COAD remain to be fully understood.

Prediction of Myocardial Infarction Using a Combined Generative Adversarial Network Model and Feature-Enhanced Loss Function.

Accurate risk prediction for myocardial infarction (MI) is crucial for preventive strategies, given its significant impact on global mortality and morbidity. Here, we propose a novel deep-learning approach to enhance the prediction of incident MI cases by incorporating metabolomics alongside clinical risk factors. We utilized data from the KORA cohort, including the baseline S4 and follow-up F4 studies, consisting of 1454 participants without prior history of MI.

Longitudinal Cervical Length Measurements and Spontaneous Preterm Birth in Singleton and Twin Pregnancies.

Importance: Changes in cervical length in twin pregnancies exhibit various patterns, but it is unclear whether the mechanism underlying spontaneous preterm birth (sPTB) is consistent. The existence of detailed phenomena in singleton pregnancies is also unclear.

Objectives: To explore the different patterns in cervical length trajectories in singleton and twin pregnancies and to analyze whether the immunological mechanisms of sPTB are consistent among these cervical length patterns.

How does physical activity improve adolescent resilience? Serial indirect effects self-efficacy and basic psychological needs.

Background: Resilience is vital for improving mental health and well-being during adolescence, which is an important yet vulnerable period. Previous research has indicated that physical activity enhances individual resilience. However, limited studies have examined underlying psychological mechanisms between them.

Combined magnetic biochar and ryegrass enhanced the remediation effect of soils contaminated with multiple heavy metals.

Biochar is a very promising material for soil remediation. However, most studies mainly focus on the adsorption ability of biochar on one heavy metal, which is difficult to evaluate the actual remediation effect since soils were contaminated with multiple heavy metals. In order to improve the soil remediation efficiency, we used the joint remediation method of magnetically modified biochar and ryegrass to remediate the soil polluted by compound heavy metals (chromium, nickel, copper, zinc, arsenic and cadmium), and evaluate the effect on the process of organic carbon mineralization in polluted soils.

Inflammation-associated D-dimer predicts neurological outcome of recent small subcortical infarct: A prospective clinical and laboratory study.

Objective: Elevated level of D-Dimer often indicates a worse prognosis in cerebral infarction. However, there is limited research on this impact within recent small subcortical infarction (RSSI). We aim to explore the role of inflammation and the total magnetic resonance imaging (MRI) burden of cerebral small vessel disease (cSVD) in this process.

Evaluating the bioequivalence and safety of liraglutide injection Victoza in healthy Chinese subjects: a randomized, open, two-cycle, self-crossover phase I clinical trial.

Liraglutide is an acylated glucagon-like peptide-1 (GLP-1) analog, and its pharmacokinetic and pharmacodynamic properties as a GLP-1 receptor (GLP-1R) agonist make it an important therapeutic option for many patients with type 2 diabetes mellitus. This study compared the bioequivalence and safety of liraglutide with the originator product in healthy Chinese adult subjects. Subjects (N = 36, both sexes) were randomized in a 1:1 ratio into two groups (18 cases each) for a two-cycle, self-crossover trial.