The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice.

Exendin-4 is a glucagon-like polypeptide-1 (GLP-1) analog derived from lizard venom, but its short half-life affects drug administration compliance. An anti-HSA nanobody with a smaller size to guide the peptide coupling to Human Serum Albumin(HSA) in vivo may be a feasible strategy for constructing inexpensive, long-acting exendin-4 analogs. For this purpose, a fusion protein (exendin-4-(G4S)3-sdAbHSA), in which a humanized anti-HSA nanobody to the C-terminal of exendin-4 through the (GlySer) flexible joint, was constructed.