Tetrahedral DNA-linked aptamer-antibody-based sandwich-type electrochemical sensor with Ag@Au core-shell nanoparticles as a signal amplifier for highly sensitive detection of α-fetoprotein.

The conventional electrochemical detection strategy for alpha-fetoprotein (AFP) is limited by the antigen-antibody (Ag-Ab) reactions and suffers from low sensitivity and poor reproducibility due to the inconsistency of Ab-modified electrodes. Herein, we designed and explored a sandwich-type electrochemical sensor for highly sensitive detection of AFP based on aptamer (Apt)-AFP-Ab interaction mode with silver@gold (Ag@Au) core-shell nanoparticles (NPs) as a signal amplifier. AuNPs were electrodeposited onto MXene (TiCT)-modified glassy carbon electrode (GCE) to get AuNPs/MXene/GCE and further used as the signal amplification substrate.

Hemin/G-quadruplex and AuNPs-MoS based novel dual signal amplification strategy for ultrasensitively sandwich-type electrochemical thrombin aptasensor.

In this work, a novel sandwich-type electrochemical aptasensor based on the dual signal amplification strategy of hemin/G-quadruplex and AuNPs-MoS was designed and constructed, which realized the highly sensitive and specific detection of thrombin (TB). In this aptasensor, the 15-mer TB-binding aptamer (TBA-1) modified with thiol group was immobilized on the surface of AuNPs modified glassy carbon electrode (AuNPs/GCE) as capturing elements. Another thiol-modified 29-mer TB-binding aptamer (TBA-2) sequence containing G-quadruplex structure for hemin immobilization was designed.

Three-dimensional MoS-graphene aerogel nanocomposites for electrochemical sensing of quercetin.

A facile and novel electrochemical sensing platform is reported for quercetin determination with MoS nanoflowers-3D graphene aerogel (3D MoS-GA) nanocomposite as signal amplified material. The 3D MoS-GA nanocomposite was synthesized through a two-step hydrothermal method, in which MoS nanoflowers were prepared in advance. Characterizations of 3D MoS-GA were performed by scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy.

Large-scale defect-rich iron/nitrogen co-doped graphene-based materials as the excellent bifunctional electrocatalyst for liquid and flexible all-solid-state zinc-air batteries.

Defect-engineering in transition-metal-doped carbon-based catalyst plays an essential role for improving the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) performance. Herein, we report a ball-milling induced defect assisted with ZnCl strategy for fabricating defect-rich iron/nitrogen co-doped graphene-based materials (Fe-N-G). The substantial mechanical shear forces and the constant corrosion to the carbon matrix by ZnCl lead to the creation of abundant defects in graphene-based materials, which facilitates doping for heteroatoms.

Poor Prognosis of Pulmonary Adenosquamous Carcinoma with and Double Mutation.

RAS mutations constitute one of the major tumorigenic mechanisms and are detected in approximately 20% of lung cancers. The most frequent mutated and well-studied RAS isoform is , which is associated with an overall poor prognosis in non-small-cell lung cancer (NSCLC). However, the clinical significances of and in NSCLC are rarely reported.

Association between Physical Literacy and Physical Activity: A Multilevel Analysis Study among Chinese Undergraduates.

Aim: To examine the association between the perceived physical literacy (PL) and physical activity (PA) levels among Chinese undergraduates.

Methods: Simplified Chinese version of the Perceived Physical Literacy Instrument and the International Physical Activity Questionnaire were used to measure 536 students' perceived PL and PA levels. Pearson's product-moment correlation and multiple linear regression were then used to examine the relationship between the perceived PL and PA levels.

Highly Exposed Active Sites of Fe/N Co-doped Defect-rich Graphene as an Efficient Electrocatalyst for Oxygen Reduction Reaction.

A defect-rich interconnected hierarchical three-dimensional Fe and N co-doped graphene has been prepared by a facile synthesis with poly (2,5-benzimidazole) (ABPBI) as nitrogen and carbon sources and CaCO as the template. ABPBI possesses abundant nitrogen, and pyrolysis of ABPBI is helpful to form graphene structure. CaCO and its decomposition products CO can promote the formation of interconnected hierarchical porous three-dimensional graphene, which possesses more defects and exposed active sites.

Circulating tumor DNA detection is correlated to histologic types in patients with early-stage non-small-cell lung cancer.

Objectives: Circulating tumor DNA (ctDNA) testing in plasma in patients with non-small-cell lung cancer (NSCLC) has the potential to be a supplemental or surrogate tool for tissue biopsy. Detection of genomic abnormalities in ctDNA and their association with clinical characteristics in early-stage NSCLC need to be clarified.

Materials And Methods: Here, we comprehensively analyzed gene variations of 48 tumor tissues and 48 matched preoperative (pre-op) plasma and 25 postoperative (post-op) plasma from early-stage NSCLC patients using a targeted 546 genes capture-based next generation sequencing (NGS) assay.

Three-dimensional reduced graphene oxide aerogel modified electrode for the sensitive quercetin sensing and its application.

Quercetin belongs to flavonoid drug that has favorable properties such as antiviral, anticancer, anti-allergic and anti-tumor. Therefore a sensitive method is highly required for quercetin determination. In this paper, a three-dimensional reduced graphene oxide aerogel (3D-rGA) with excellent porous framework was synthesized via one-step hydrothermal technique.

A graphene modified carbon ionic liquid electrode for voltammetric analysis of the sequence of the Staphylococcus aureus nuc gene.

The authors describe a voltammetric method for the detection of the nuc ssDNA sequence originating from Staphylococcus aureus by using a carbon ionic liquid electrode modified with electrodeposited three-dimensional graphene (3DGR). Probe ssDNA was electrostatically adsorbed on the modified electrode by a potentiostatic method. The porous structure and large surface area of 3DGR greatly increase the amount of immobilized probe ssDNA on the interface, which is beneficial for the reaction with target ssDNA.

Investigation on direct electrochemical and electrocatalytic behavior of hemoglobin on palladium-graphene modified electrode.

Palladium-graphene (Pd-GR) nanocomposite was acted as modifier for construction of the modified electrode with direct electrochemistry of hemoglobin (Hb) realized. By using Nafion as the immobilization film, Hb was fixed tightly on Pd-GR nanocomposite modified carbon ionic liquid electrode. Electrochemical behaviors of Hb modified electrode were checked by cyclic voltammetry and a pair of redox peaks originated from direct electron transfer of Hb was appeared.

The Gα1-cAMP signaling pathway controls conidiation, development and secondary metabolism in the taxol-producing fungus Pestalotiopsis microspora.

G-protein-mediated signaling pathways regulate fungal morphogenesis, development and secondary metabolism. In this study, we report a gene, pgα1, that putatively encodes the α-subunit of a group I G protein in Pestalotiopsis microspora NK17, which is known to produce various secondary metabolites, including the antitumor drug taxol and pestalotiollide B (PB). Mutants of pgα1 showed retarded vegetative growth, aging of the mycelium, premature conidiation, deformed conidia, significantly increased melanin production, and a sharp decrease in PB production.

A Putative Histone Deacetylase Modulates the Biosynthesis of Pestalotiollide B and Conidiation in Pestalotiopsis microspora.

Fungi of the genus Pestalotiopsis have drawn attention for their capability to produce an array of bioactive secondary metabolites that have potential for drug development. Here, we report the determination of a polyketide derivative compound, pestalotiollide B, in the culture of the saprophytic fungus Pestalotiopsis microspora NK17. Structural information acquired by analyses with a set of spectroscopic and chromatographic techniques suggests that pestalotiollide B has the same skeleton as the penicillide derivatives, dibenzodioxocinones, which are inhibitors of cholesterol ester transfer protein (CETP), and as purpactins A and C', inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT).

Voltammetric investigation on interaction of protein with chromotrope 2R and its analytical application.

The electrochemical behaviors of the interaction of chromotrope 2R (CH2R) with human serum albumin (HSA) are investigated on the hanging mercury drop electrode with linear sweep voltammetry. In the acidic buffer solution (pH 2.5) CH2R has a well-defined voltammetric reductive wave at -0.

[Fading spectrophotometric determination of heparin with brilliant cresyl blue].

A new fading spectrophotometric method for determining trace heparin is proposed in the present paper. In pH 3.0 Britton-Robinson (B-R) buffer solution, brilliant cresyl blue can react with heparin resulting in the decrease of absorbance of BCB at 592 nm.