Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy.

Stem cell apoptosis exists widely in embryonic development, tissue regeneration, repair, aging and pathophysiology of disease. The molecular mechanism of stem cell apoptosis has been extensively investigated. However, alterations in biomechanics and nanomorphology have rarely been studied.

Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells.

Background: Uniaxial/biaxial tensile stress has been employed to induce chondrocyte differentiation of mesenchymal stem cells. However, the effects of radial tensile stimuli on differentiation of MSCs into fibrocartilage remain unclear.

Results: It was found that induced bone marrow mesenchymal stem cells (BMSCs) were not only similar to TMJ disc cells in morphology, but also could synthesize type I collagen (Col I), a small amount of type II collagen (Col II) and glycosaminoglycans (GAGs).

Feasibility of peaking carbon emissions of the power sector in China's eight regions: decomposition, decoupling, and prediction analysis.

Carbon emissions in the power sector are an important part of China's total carbon emissions and have a significant impact on whether China can achieve the 2030 carbon peak target. Based on the three perspectives of decomposition, decoupling, and prediction, this paper studies the feasibility of carbon emission peaks in eight major regional power sectors in China. First, the generalized Divisia index model (GDIM) is used to decompose the carbon emissions of the eight regional power sectors, and the driving factors and their effects on carbon emissions in the power sector of each region are compared.

Mechanical, nanomorphological and biological reconstruction of early‑stage apoptosis in HeLa cells induced by cytochalasin B.

There is a growing interest in the fact that mechanical signals may be as important as biological signals in evaluating cell viability. To investigate the alterations in biomechanics, nanomorphology and biological apoptotic signals during early apoptosis, an apoptosis model was established for cervical cancer HeLa cells induced by cytochalasin B (CB). The cellular mechanical properties, geometry, morphology and expression of key apoptotic proteins were systematically analyzed.

40-Fold Enhanced Intrinsic Proton Conductivity in Coordination Polymers with the Same Proton-Conducting Pathway by Tuning Metal Cation Nodes.

Three isostructural imidazole-cation-templated metal phosphates (FJU-25) are the first examples to demonstrate that the tuning of metal cation nodes can be an efficient strategy to significantly improve the proton conductivity without changing the structure of the proton-conducting pathway.

[Topography and mechanical property of goat temporomandibular joint disc cells].

Objective: This study is performed to investigate the cell topographies and biomechanical properties of two different types of temporomandibular joint (TMJ) discs from goats by using JPK Nano Wizard 3 biological atomic force microscopy (AFM). This process provides a guideline for selecting seed cells for TMJ disc tissue engineering.

Methods: TMJ disc cells from primary goats were cultured by monolayer culture method.

Peroxisome proliferator-activated receptor alpha expression changes in human pregnant myometrium.

Peroxisome proliferator-activated receptor alpha (PPARα) has been demonstrated to exhibit anti-inflammatory activities that are hypothesized to play a key role in labor suppression and maintenance of uterine quiescence. The aim of this study was to identify pregnancy- and labor-associated changes in PPARα in human myometrium. For this investigation, human myometrium was obtained from premenopausal women, and the study participants were categorized into the following 4 groups: nonpregnant (NP; n = 10), preterm not in labor (PNL; n = 10, gestation range 20-35 weeks), term not in labor (TNL; n = 20, gestation range 37-41 weeks), and term in labor (TL; n = 20, gestation range 37-41 weeks).

[Effects of basic fibroblast growth factor on bone marrow mesenchymal stem cell differentiation into temporomandibular joint disc cells].

The present paper is aimed to observe the effects of basic fibroblast growth factor (bFGF) on bone marrow mesenchymal stem cell (BMSCs) differentiation. The bFGF was used to stimulate BMSCs and histology, immunohistochemistry and enzyme linked immunosorbent assay (ELISA) were used to examine the extracellular matrix produced by induced BMSCs, evaluated the feasibility of BMSCs being the seeding cells of temporomandibular joint (TMJ) disc tissue engineering. The results showed that having been induced with bFGF, the BMSCs could differentiate into fibroblast-like cells, which could synthesize GAG and collagen type I matrix.

[Cell sources for engineered temporomandibular joint disc tissue: present and future].

The purpose of this review is to provide a reference for researchers in investigating the tissue engineering of the temporomandibular joint (TMJ) disc. Currently tissue engineering of the TMJ disc is in its infancy, and cell source is one of the key factors that define the development of the tissue engineering of TMJ disc. In this paper, 6 kinds of cells: the TMJ disc native cells, chondrocytes, dermal fibroblasts, bone marrow-derived mesenchymal stem cells, adipose-derived stem cells, and embryonic stem cells are introduced.