Establishment of a platform based on dual RPA combined with CRISPR/Cas12a for the detection of and its KPC resistance gene.

Carbapenem resistant (CRKP) can cause serious hospital- and community-acquired infections. Treatment for CRKP infection is limited, resulting in prolonged hospitalization and high consultation costs. The KPC genotype has the highest detection rate of CRKP, and its mortality rate is higher than the overall mortality rate of CRKP.

The Impact of miR-34a on Endothelial Cell Viability and Apoptosis in Ischemic Stroke: Unraveling the -Homocysteine Pathway.

Introduction: Ischemic stroke (IS) is a global health concern, often tied to dyslipidemia and vascular endothelial dysfunction. MicroRNA-34a (miR-34a) was reported to be up-regulated in the blood samples of patients with IS, but the specific role of miR-34a and methylenetetrahydrofolate reductase (MTHFR) in IS remains to be elucidated.

Methods: We studied 143 subjects: 71 IS patients, and 72 healthy controls.

Investigation of genomic and pathogenicity characteristics of ST1 human strains from Guangxi Zhuang Autonomous Region (GX) between 2005 and 2020 in China.

is a significant and emerging zoonotic pathogen. ST1 and ST7 strains are the primary agents responsible for human infections in China, including the Guangxi Zhuang Autonomous Region (GX). To enhance our understanding of ST1 population characteristics, we conducted an investigation into the phylogenetic structure, genomic features, and virulence levels of 73 ST1 human strains from GX between 2005 and 2020.

A dual RPA-LFD assay for the simultaneous detection of and .

was one of the most common bacteria that caused foodborne illness, with () and () infections accounting for more than 75% of human salmonella infections. In this study, we developed a method of dual recombinase polymerase amplification (RPA) combined with a lateral flow dipstick for the rapid detection of and in clinical specimens (stool). The entire reaction process, including amplification and result reading, could be completed within 65 min.

A multiplex RPA coupled with CRISPR-Cas12a system for rapid and cost-effective identification of carbapenem-resistant .

Background: Carbapenem-resistant (CRAB) poses a severe nosocomial threat, prompting a need for efficient detection methods. Traditional approaches, such as bacterial culture and PCR, are time-consuming and cumbersome. The CRISPR-based gene editing system offered a potential approach for point-of-care testing of CRAB.

Analysis of carbapenem-resistant carbapenemase gene distribution and biofilm formation.

Objective: In recent years, has been appearing in hospitals with high drug resistance and strong vitality, which brings many difficulties to clinical treatment. In this study, 255 strains of were isolated from Youjiang Medical University for Nationalities Affiliated Hospital clinical samples and found to be highly resistant to carbapenems. The drug resistance, biofilm-forming ability, and carbapenase gene distribution of 145 carbapenem-resistant (CRAB) strains were analyzed statistically.

, an Emerging Zoonotic Pathogen, Possesses the Capacity to Induce Cerebral Inflammatory Responses.

To date, three strains, BS26, BS27, and NN1, have been isolated from the blood cultures of patients with peritonitis, pneumonia, and arthritis, indicating that is an emerging threat to susceptible people. There is thus an urgent need to further evaluate the pathogenesis of clinical strains in order to design efficient anti-inflammatory strategies. Our previous study demonstrated the capacity of clinical strains to enter the central nervous system (CNS) of infected mice.

Comparative Genomics Reveal the Utilization Ability of Variable Carbohydrates as Key Genetic Features of Pathogens in Their Pathogenic Lifestyles.

Background: and , the only two pathogens of , can survive in various environments, having different pathogenic characteristics. However, the genetic basis of their excellent adaptability and differences in pathogenicity has still not been completely elucidated.

Methods: We performed a comparative genomic analysis based on 275 , 10 and 22 non-pathogenic strains.

Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.

After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people's lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid testing methods. Among several common isothermal amplification methods like displaced amplification, rolling circle amplification, and so on, recombinase polymerase amplification (RPA) was recently paid more attention to.

Investigation of the Genomic and Pathogenic Features of the Potentially Zoonotic .

Recently, reference strains of serotype 20, 22, and 26 were reclassified as . The public health significance of is underestimated due to the lack of clinical isolates. In the present study, we first reported two sporadic infections in humans, after using full-length 16S rRNA and housekeeping genes' phylogeny and ANI values of genome sequence comparisons to determine the species of their isolates BS26 and BS27.