Recognition of hyperacetylated N-terminus of H2AZ by TbBDF2 from .

Histone modification plays an important role in various biological processes, including gene expression regulation. Bromodomain, as one of histone readers, recognizes specifically the ε--lysine acetylation (KAc) of histone. Although the bromodomains and histone acetylation sites of (), a lethal parasite responsible for sleeping sickness in human and nagana in cattle, have been identified, how acetylated histones are recognized by bromodomains is still unknown.

Platinum transfer from hCTR1 to Atox1 is dependent on the type of platinum complex.

In spite of their wide application, the cellular uptake of platinum based anticancer drugs is still unclear. The copper transport protein, hCTR1, is proposed to facilitate the cellular uptake of cisplatin, whereas organic cation transport (OCT) is more important for oxaliplatin. It has been reported that both N-terminal and C-terminal metal binding motifs of hCTR1 are highly reactive to cisplatin, which is the initial step of protein assisted cellular uptake of cisplatin.

A comparative study on the interactions of human copper chaperone Cox17 with anticancer organoruthenium(II) complexes and cisplatin by mass spectrometry.

Herein we report investigation of the interactions between anticancer organoruthenium complexes, [(η(6)-arene)Ru(en)(Cl)]PF6 (en=ethylenediamine, arene=p-cymene (1) or biphenyl (2)), and the human copper chaperone protein Cox17 by mass spectrometry with cisplatin as a reference. The electrospray ionization mass spectrometry (ESI-MS) results indicate much weaker binding of the ruthenium complexes than that of cisplatin to apo-Cox172s-s, the functional state of Cox17. Up to tetra-platinated Cox17 adducts were identified while only mono-ruthenated and a little amount of di-ruthenated Cox17 adducts were detected even for the reactions with 10-fold excess of the Ru complexes.

The reaction of a platinated methionine motif of CTR1 with cysteine and histidine is dependent upon the type of precursor platinum complex.

The human copper protein (hCTR1) is believed to facilitate the cellular uptake of cisplatin. Cisplatin likely binds to the methionine (Met)-rich motifs located in the N-terminus of hCTR1, and ligand exchange would be essential if cisplatin has to pass through the hCTR1 channel. In this work, we investigated the reaction between platinated adducts of a methionine-rich motif of yeast CTR1 (Mets7) and N-acetyl-cysteine (AcCys) or N-acetyl-histidine (AcHis), mimicking metal-binding residues downstream the CTR1 channel.

The safe and efficient use of forced-air warming systems.

Maintaining perioperative normothermia is important to ensure that a patient does not experience inadvertent hypothermia and its consequences, such as increased blood loss, cardiac abnormalities, prolonged recovery, and increased risk for wound infection. Many clinical guidelines recommend the use of forced-air warming as one of several techniques to prevent inadvertent perioperative hypothermia. Safe use of forced-air warming devices includes choosing the right device, assessing the patient for risks, protecting the patient from burn injuries, appropriately maintaining the patient's body temperature, and using the device as directed by the manufacturer's recommendations.

[Observation on analgesia effect of electroacupuncture during gynecologic outpatient operation].

Objective: To verify the feasibility of electroacupuncture analgesia applied to gynecologic outpatient operation.

Methods: Two hundred patients were randomly divided into an electroacupuncture analgesia group and an intravenous anesthesia group, 100 cases in each group. Operation types included artificial abortion, diagnostic curettage and remove of intrauterine divice.

Contributions of functional groups and extracellular polymeric substances on the biosorption of dyes by aerobic granules.

The contributions of loosely bound extracellular polymeric substances (LB-EPS), tightly bound EPS (TB-EPS), residual sludge (the sludge left after EPS extraction) and functional groups such as amine, carboxyl, phosphate and lipid on aerobic granules on biosorption of four different dyes (Reactive Brilliant Blue KN-R (KN-R), Congo Red (CR), Reactive Brilliant Red K-2G (RBR) and Malachite Green (MG)) were investigated. EPS may be responsible for biosorption of cationic dyes. However, residual sludge always made greater contribution than that of EPS.

[Sludge population optimisation in biological wastewater treatment systems through on-line control].

The structure and function of activated sludge community were studied in an A/O pilot-scale plant treating actual domestic wastewater. FISH results show the feasibility and effectiveness of sludge population optimisation. A/O process short-cut nitrification with process control of DO and aeration based on DO and pH on-line sensors can be achieved, and gradually eliminated nitrite oxidising bacteria, and therefore achieved nitrogen removal via the nitrite pathway.

[Short-cut/Complete nitrification and denitrification in a pilot-scale plant treating actual domestic wastewater].

It is realized short-cut nitrification and denitrification in an A/O pilot-scale plant treating actual domestic wastewater controlling DO concentration (0.5 mg/L) in low level at normal temperature, the average nitrite accumulation rate reached 85 % or above. Three operational modes: low DO short-cut nitrification and denitrification (Mode 1), low DO complete nitrification and denitrification (Mode 2) and high DO complete nitrification and denitrification (Mode 3) were briefly compared, based on the nitrogen removal, operating cost, sludge characteristics and operational mechanism.

[Experimental study of nitrite accumulation in pre-denitrification biological nitrogen removal process].

It was realized nitrite accumulation in a pilot-scale pre-denitrification process at normal temperature. The influence factors, temperature, pH, FA and DO, for nitrite accumulation were analyzed. The nitrite was accumulated in the beginning time with the DO kept at 0.