Modulation of the magnetic properties of mononuclear Dy(III) complexes by tuning the coordination geometry and local symmetry.

Precise control of the crystal field and local symmetry around the paramagnetic spin center is crucial for the design and synthesis of single-molecule magnets (SMMs). Herein, three mononuclear Dy(III)-based complexes, [Dy(L)(CHCOO)](BPh)(CHCl) (1), [Dy(L)(2,6-Cl-4-NO-PhO)(HO)](PF)(HO)(2,6-Cl-4-NO-PhO) (2) and [Dy(L)(2,6-Cl-4-NO-PhO)](BPh)(CHCl) (3) (L = N6-hexagonal plane accomplished by a neutral Schiff base ligand formed from 2,6-diacetylpyridine and ethylenediamine), are successfully isolated. In these complexes, the Dy(III) centers are coordinated with six neutral N atoms from a nonrigid equatorial ligand, while different oxygen-bearing ligands are arranged at the axial positions of the central ions by gradual regularization of the axial ligands.

MicroRNA-483-5p accentuates cisplatin-induced acute kidney injury by targeting GPX3.

The ability of cisplatin (cis-diamminedichloroplatinum II) toxicity to induce acute kidney injury (AKI) has attracted attention and concern for a long time, but the molecular mechanism of action for cisplatin is not clear. MicroRNA-483 is involved in several diseases, such as tumorigenesis and osteoarthritis, but its renal target and potential role in AKI are unknown. In this study, we explored the pathogenic role and underlying mechanism of miR-483-5p in cisplatin-induced AKI, using transgenic mice, clinical specimen, and in vitro cell line.

[Estradiol significantly increases the expression of antioxidant enzymes in osteoporotic rats and osteoblasts in vitro].

Objective: To investigate the effect of estradiol on the expression of antioxidant enzymes in osteoblasts and its role in postmenopausal osteoporosis.

Methods: Rat models of osteoporosis established by ovariectomy were treated with estradiol for 3 months, and the changes in serum levels of reactive oxygen species (HO) and antioxidant enzymes (γ -GCS, GSH-ST and GSH-px) were detected. The effects of estradiol on the expression of γ -GCS mRNA and protein in osteoblast-like cells MC3T3-E1, MG63 and OB were examined with PCR and Western blotting.

DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis.

Background/aims: The mechanistic target of rapamycin (mTOR) signaling pathway is essential for angiogenesis and embryonic development. DEP domain-containing mTOR-interacting protein (DEPTOR) is an mTOR binding protein that functions to inhibit the mTOR pathway In vitro experiments suggest that DEPTOR is crucial for vascular endothelial cell (EC) activation and angiogenic responses. However, knowledge of the effects of DEPTOR on angiogenesis in vivo is limited.

[A method for efficient transduction of miR-483-5p in the kidney of mice].

Objective: To establish a method for gene delivery in murine renal tissue using lentivirus vector encoding miR-483-5p.

Methods: Thirty-five C57BL/6J mice were randomly divided into control group, low-dose treatment group (5 µL each kidney) , and high?dose treatment group (20 µL each kidney), and in the latter two groups, the lentivirus vector encoding miR-483-5p were injected in the renal cortex. The tissue samples were collected at 7 and 21 days after the injection.

[Cloning, mutagenesis and symbiotic phenotype of three lipid transfer protein encoding genes from Mesorhizobium huakuii 7653R].

Objective: Lipid transfer protein superfamily is involved in lipid transport and metabolism. This study aimed to construct mutants of three lipid transfer protein encoding genes in Mesorhizobium huakuii 7653R, and to study the phenotypes and function of mutations during symbiosis with Astragalus sinicus.

Methods: We used bioinformatics to predict structure characteristics and biological functions of lipid transfer proteins, and conducted semi-quantitative and fluorescent quantitative real-time PCR to analyze the expression levels of target genes in free-living and symbiotic conditions.

Treatment of 140 cerebral palsied children with a combined method based on traditional Chinese medicine (TCM) and western medicine.

Objective: To observe and evaluate a method that is effective and practical for treatment of cerebral palsied (CP) children in China.

Method: The patient's age and disease type and individual specific conditions were considered in choosing therapy methods accordingly: Chinese herbs, acupuncture, auricular seed pressure, point finger pressing, massage, orthopedic hand manipulation, physiotherapy, occupational therapy, language therapy, etc. Meanwhile we created a new CP treatment model that combines hospitalized treatment with family therapy.

[Follow-up study of mental developments in high-risk children].

Objective: To study the mental developments in high risk children and the impact of the high risk factors on neurologic abnormalities, mental defect and long-term outcome.

Methods: The mental development of 122 children who had been exposed to high-risk factors and treated between March 1994 to May 1995 during their newborn periods was evaluated. Gesell development scales were performed when they were at 6 and 12 months old.

[A case-control study of risk factors for childhood cerebal palsy].

OBJECTIVE: To identify possible risk factors for cerebral palsy (CP) in children. METHODS: A Population-based survey was conducted (including 92 CP cases) in 66 townships of 15 cities of Zhejiang Province from October to November, 1998. 184 of matched controls were selected for comparison.