A New Phospholipase D from sp. JT01: Biochemical Characterization, Crystallization and Application in the Synthesis of Phosphatidic Acid.

A new phospholipase D from marine sp. JT01 (MsPLD) was recombinantly expressed and biochemically characterized. The optimal reaction temperature and pH of MsPLD were determined to be 35 °C and 8.

Improving Both the Thermostability and Catalytic Efficiency of Phospholipase D from sp. JT01 through Disulfide Bond Engineering Strategy.

Mining of Phospholipase D (PLD) with high activity and stability has attracted strong interest for investigation. A novel PLD from marine sp. JT01 (MsPLD) was biochemically and structurally characterized in our previous study; however, the short half-life time () under its optimum reaction temperature seriously hampered its further applications.

Crystal Structure of a Phospholipase D from the Plant-Associated Bacteria Strain AS9 Reveals a Unique Arrangement of Catalytic Pocket.

Phospholipases D (PLDs) play important roles in different organisms and in vitro phospholipid modifications, which attract strong interests for investigation. However, the lack of PLD structural information has seriously hampered both the understanding of their structure-function relationships and the structure-based bioengineering of this enzyme. Herein, we presented the crystal structure of a PLD from the plant-associated bacteria strain AS9 (SpPLD) at a resolution of 1.

[Sources and Distribution of Phosphorus in Sediments of the Jinpen Reservoir].

In order to explore the sources and distribution of phosphorus in sediments of a stratified water source reservoir, the total phosphorus (TP) content and distribution of phosphorus fractions in sedimentation particles and sediments of the Jinpen Reservoir of Xi'an were analyzed from March to November in 2017. The results showed that the TP content in the surface sediments of the Jinpen Reservoir was obviously affected by the deposition of particulate phosphorus (PP), and the correlation coefficient was 0.8775.

[Migration Characteristics of Manganese During Rainfall Events and Its Impacts on Water Quality in a Drinking Water Source Reservoir].

In view of the problem of excessive manganese concentrations in the Xi'an Jinpen Reservoir during the flood season in 2017, the vertical distribution of manganese in density currents and its occurrence pattern were monitored at multiple monitoring sections along the upstream reaches to the main basin. The influences of density currents plunging into the reservoir on the migration and transformation of Mn were studied, and sedimentation, output, and deposition of manganese in the reservoir water were also specifically estimated during a single, typical storm runoff process. Devices for avoiding high turbidity and high load inflows in rainfall events were proposed.

Controlling reduced iron and manganese in a drinking water reservoir by hypolimnetic aeration and artificial destratification.

The concentrations of iron (Fe) and manganese (Mn) in the water column have extremely important effects on the water quality of drinking water reservoirs; however, reservoirs often experience problematic Fe and Mn levels during seasonal stratification and rainfall events. Water-lifting aerators (WLAs) were deployed in the Jinpen Reservoir to control these issues with Fe and Mn at the source via bottom aeration and artificial destratification. In this study, variations of Fe and Mn concentrations in the water column, porewater, and sediments, were used to characterize behaviors of reduced Fe and Mn under the conditions of hypolimnetic aeration and artificial destratification during periods of hypolimnion hypoxia and rainfall events.

LPE-1, an orally active pyrimidine derivative, inhibits growth and mobility of human esophageal cancers by targeting LSD1.

Histone lysine specific demethylase 1 (LSD1) plays an important role in epigenetic modifications, and aberrant expression of LSD1 predicts tumor progression and poor prognosis in human esophageal cancers. In this study, a series of LSD1 inhibitors were synthesized and proved to be highly potent against human esophageal squamous cell carcinoma (ESCC). Our data showed that these LSD1 inhibitors selectively suppressed the viability of esophageal cancer cell line (EC-109) bearing overexpressed LSD1.

Efficacy and safety of AIR inhaled insulin compared to insulin lispro in patients with type 1 diabetes mellitus in a 6-month, randomized, noninferiority trial.

Background: Patients with type 1 diabetes may prefer features of AIR inhaled insulin (developed by Alkermes, Inc. [Cambridge, MA] and Eli Lilly and Company [Indianapolis, IN]; AIR is a registered trademark of Alkermes, Inc.) over insulin injection, but the two methods need to be compared for efficacy and safety.