Stochastic optical reconstruction microscopy (STORM) is extensively utilized in the fields of cell and molecular biology as a super-resolution imaging technique for visualizing cells and molecules. Nonetheless, the imaging process of STORM is frequently susceptible to noise, which can significantly impact the subsequent image analysis. Moreover, there is currently a lack of a comprehensive automated processing approach for analyzing protein aggregation states from a large number of STORM images.
View Article and Find Full Text PDFTDLAS (tunable diode laser absorption spectroscopy) is an important gas analysis method that can be employed to obtain characteristic parameters non-invasively by the infrared absorption spectra of tracer molecules such as CH, HO and O. In this study, a portable HO-based TDLAS system with a dual optical path was developed with the aim of assessing the combustion characteristics of flammable gases. Firstly, a calculation method of gas characteristics including temperature and velocity combining absorption spectra and a HITRAN database was provided.
View Article and Find Full Text PDFConcerning the issues regarding driving mileage reduction for electric vehicles (EVs) in cold climates, a heat pump system with low global warming potential refrigerant R290/R1234yf is employed as one of the promising solutions. Different from the widely used mobile refrigerant R134a, R290 and R1234yf are both flammable or explosive. The application of R290/R1234yf in the mobile heat pump system is hindered by unexpected refrigerant leakage with the existence of fire and explosion risk.
View Article and Find Full Text PDFThe accidental leakage is one of the main risks during the pipeline transportation of high pressure CO2. The decompression process of high pressure CO2 involves complex phase transition and large variations of the pressure and temperature fields. A mathematical method based on the homogeneous equilibrium mixture assumption is presented for simulating the leakage flow through a nozzle in a pressurised CO2 pipeline.
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