Localization of tumor by chemiluminescence probe during photosensitization action.

As a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-[2-(N'-(5-fluoresceinyl) thioureido)ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one dosium salt (FCLA) can sensitively and specifically react with singlet oxygen ((1)O(2)) and superoxide (O(2)(-)). Based on the fact that photosensitization action involves (1)O(2) generation, this report presents a novel method for localization of tumor mediated by FCLA. In experiments, FCLA was used as an optical reporter of (1)O(2) produced from photosensitization reaction of hematoporphyrin derivative.

Experimental study on photodynamic diagnosis of cancer mediated by chemiluminescence probe.

A novel method of photodynamic diagnosis of cancer mediated by chemiluminescence probe is presented. The mechanism for photodynamic therapy involves singlet oxygen ((1)O(2)) generated by energy transfer from photosensitizers. (1)O(2) can react with 3,7-dihydro-6-[4-[2-(N'-(5-fluoresceinyl)thioureido)ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one sodium salt (FCLA), which is a Cypridina luciferin analog and a specific chemiluminescence probe for detecting (1)O(2) and superoxide (O(2)(-)).