Effects of moderate intensity exercise on liver metabolism in mice based on multi-omics analysis.

Physical exercise is beneficial to keep physical and mental health. The molecular mechanisms underlying exercise are still worth exploring. The healthy adult mice after six weeks of moderate-intensity exercise (experimental group) and sedentary mice (control group) were used to perform transcriptomic, proteomic, lactylation modification, and metabolomics analysis.

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs.

In the early stage of embryonic development, the neural tube (NT) cannot be closed properly due to some complex factors, including environmental factors, genetic factors, and the relationship between various factors, leading to the occurrence of neural tube defects (NTDs). In this study, we induced a mouse model of NTDs by feeding mice with a low-folate diet and intraperitoneally injecting them with 1.5 mg/kg methotrexate on E7.

Acetylome analyses provide novel insights into the effects of chronic intermittent hypoxia on hippocampus-dependent cognitive impairment.

Introduction: Chronic intermittent hypoxia (CIH) can negatively affect hippocampal function through various molecular mechanisms. Protein acetylation, a frequently occurring modification, plays crucial roles in synaptic plasticity and cognitive processes. However, the global protein acetylation induced by CIH in the hippocampus and its specific effects on hippocampal function and behavior remain poorly understood.

Altered dynamic functional and effective connectivity in drug-naive children with Tourette syndrome.

Tourette syndrome (TS) is a developmental neuropsychiatric disorder characterized by repetitive, stereotyped, involuntary tics, the neurological basis of which remains unclear. Although traditional resting-state MRI (rfMRI) studies have identified abnormal static functional connectivity (FC) in patients with TS, dynamic FC (dFC) remains relatively unexplored. The rfMRI data of 54 children with TS and 46 typically developing children (TDC) were analyzed using group independent component analysis to obtain independent components (ICs), and a sliding-window approach to generate dFC matrices.

Intergenerational association of gut microbiota and metabolism with perinatal folate metabolism and neural tube defects.

Disorders of folic acid metabolism during pregnancy lead to fetal neural tube defects (NTDs). However, the mechanisms still require further investigation. Here, we aim to analyze the brain metabolic profiles of 30 NTDs and 30 healthy fetuses.

Multi-omics analysis explores the effect of chronic exercise on liver metabolic reprogramming in mice.

The effect of exercise on human metabolism is obvious. However, the effect of chronic exercise on liver metabolism in mice is less well described. The healthy adult mice running for 6 weeks as exercise model and sedentary mice as control were used to perform transcriptomic, proteomic, acetyl-proteomics, and metabolomics analysis.

Disturbed microbiota-metabolites-immune interaction network is associated with olfactory dysfunction in patients with chronic rhinosinusitis.

Purpose: Olfactory dysfunction (OD) is a debilitating symptom frequently reported by patients with chronic rhinosinusitis (CRS) and it is associated with a dysregulated sinonasal inflammation. However, little information is available about the effect of the inflammation-related nasal microbiota and related metabolites on the olfactory function in these patients. Therefore, the current study aimed to investigate the nasal microbiota-metabolites-immune interactions and their role in the pathogenesis of OD in CRS patients.

Folate-deficiency induced acyl-CoA synthetase short-chain family member 2 increases lysine crotonylome involved in neural tube defects.

Maternal folate deficiency increases the risk of neural tube defects (NTDs), but the mechanism remains unclear. Here, we established a mouse model of NTDs low folate diets combined with MTX-induced conditions. We found that a significant increase in butyrate acid was observed in mouse NTDs brains.

Sodium butyrate mediates histone crotonylation and alleviated neonatal rats hypoxic-ischemic brain injury through gut-brain axis.

Neonatal hypoxic-ischemic encephalopathy (HIE) refers to nervous system damage caused by perinatal hypoxia, which is the major cause of long-term neuro-developmental disorders in surviving infants. However, the mechanisms still require further investigation. In this study, we found that the butanoate metabolism pathway exhibited significantly decreased and short chain fatty acid (SCFAs)-producing bacteria, especially butyrate-producing bacteria, were significantly decreased in fecal of neonatal hypoxic-ischemic brain damage (HIBD) rats.

Abnormal transcriptome-wide DNA demethylation induced by folate deficiency causes neural tube defects.

Neural tube defect (NTDs) is one of the most common and serious fetal and neonatal birth defects. Neural tube closure (NTC) is an exquisitely coordinated process and this procedure is influenced by both genetic and environmental factor. Folic acid (FA) supplementation is an effective for prevention of a proportion of NTDs, however, the mechanism remains unclear.

Quantification of the carbon released by a marine fish using a carbon release model and radiocarbon.

Here we propose a carbon release model that divides fish-released carbon into two sources (ingested food and the fish body), and three forms (dissolved organic carbon (DOC), CO, and particulate carbon (PC)). We quantified the daily carbon budget of a marine fish Oryzias melastigma by feeding the fish radiocarbon-labeled living rotifer. We found that 91%-92%, 25%-47%, 28%-50%, 20%-31%, and 8%-9% of the ingested food carbon was absorbed, assimilated, and released as DOC, CO, and PC, respectively.

The Alternation of Gray Matter Morphological Topology in Drug-Naïve Tourette's Syndrome in Children.

Tourette syndrome (TS) is a neurodevelopment disorder characterized by motor and phonic tics. We investigated the topological alterations in pediatric TS using morphological topological analysis of brain structures. We obtained three-dimensional T1-weighted magnetic resonance imaging (MRI) sequences from 59 drug-naïve pediatric patients with TS and 87 healthy controls.

Physiological and behavioral responses of the copepod Temora turbinata to hypoxia.

Hypoxia is intensified in tropical and subtropical estuarine and coastal waters and brings about lethal and sublethal effects to marine copepods. The physiological and behavioral responses of the subtropical calanoid copepod Temora turbinata were tested after short-term exposure to hypoxia. The LD values were 3.

Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.

Background: The long noncoding RNAs (lncRNAs) have been confirmed to be involved in sepsis-induced organ injury. Here, we first investigated the functional role and the underlying mechanism of lncRNA LINC00472 in sepsis-induced acute hepatic injury (AHI).

Methods: Human liver THLE-3 cells were treated with lipopolysaccharide (LPS) to mimic sepsis-induced AHI in vitro; intraperitoneal injection of LPS in rats were used as an in vivo model of AHI induced by sepsis.

Allocation and stoichiometric regulation of phosphorus in a freshwater zooplankton under limited conditions: Implication for nutrient cycling.

Because zooplankton is potentially limited by phosphorus (P) in freshwater, they may modify their body P distributions in different biochemical and anatomic components depending on the environmental P levels. In the present study, we quantified the distribution and regulation of P in a freshwater zooplankton Daphnia magna under P-limited conditions by using P as a radiotracer. We demonstrated that the P allocation patterns in D.

Influence of Hydrological Heterogeneity on Rotifer Community Structure in Three Different Water Bodies in Shantou Area, Guangdong (China).

Rotifers, small but essential invertebrates in aquatic ecosystems, are sensitive to environmental changes and are proposed to be indicators of trophic state. However, the effects of hydrological heterogeneity on the rotifer community and the ability of rotifer indices to reflect trophic state across different water bodies are still unclear. Here, we investigated rotifer community structure in different seasons in the three types of water bodies: Han river downstream (HD), Reservoir (RE) and Tidal creek (TC) in Shantou City, Guangdong, China.

Allocation Costs Associated with Induced Defense in Phaeocystis globosa (Prymnesiophyceae): the Effects of Nutrient Availability.

Colony enlargement in Phaeocystis globosa has been considered as an induced defense strategy that reduces its susceptibility to grazers, but allocation costs inflicted by this plastic morphological defense are poorly understood. We conducted experiments in which P. globosa cultures were exposed to chemical cues from copepods, ciliates and heterotrophic dinoflagellates, respectively, under nutrient sufficient and deficient conditions to evaluate allocation costs associated with induced defense.

[Algae identification research based on fluorescence spectral imaging technology combined with cluster analysis and principal component analysis].

In order to explore rapid real-time algae detection methods, in the present study experiments were carried out to use fluorescence spectral imaging technology combined with a pattern recognition method for identification research of different types of algae. The fluorescence effect of algae samples is obvious during the detection. The fluorescence spectral imaging system was adopted to collect spectral images of 40 algal samples.