Rapid identification of α-glucosidase inhibitors from using spectrum-effect, component knock-out, and molecular docking technique.

Instruction: Poria is known for its health-promoting effects and is consumed as a food due to its potential hypoglycemic activity. However, the composition of Poria is complex, and the bioactive compounds that inhibit α-glucosidase are not clear.

Methods: In this study, the fingerprint of the Poria methanol extract characterized by high-performance liquid chromatography (HPLC) and the model of the corresponding spectrum-effect relationship for α-glucosidase was first established to screen the active compounds from Poria.

Patrinoside and Patrinoside A from a Improve Insulin Resistance by Inhibiting NF-B, MAPK Pathways and Oxidative Stress in RAW264.7 and 3 T3-L1 Cells.

, as traditional food and medicine plant, was used to treat appendicitis, enteritis, and hepatitis for thousand years in China. Patrinoside and patrinoside A isolated from could significantly improve insulin resistance (IR) by activating PI-3 K/AKT signaling pathway in our previous study. Since IR is closely related to inflammation, their anti-inflammatory activities in RAW264.

Inhibitory effect of quercetin-3-O-α-rhamnoside, p-coumaric acid, phloridzin and 4-O-β-glucopyranosyl-cis-coumaric acid on rats liver microsomes cytochrome P450 enzyme activities.

P-coumaric acid, phloridzin, quercetin-3-O-α-rhamnoside and 4-O-β-glucopyranosyl-cis-coumaric acid isolated in Malus micromalus Makino fruit were investigated the inhibitory activity of cytochrome CYP450 enzyme by the probe test method of rat liver microsomes in vitro, and determined the role in drug metabolism and/or toxicology. Enzymatic kinetics method was used to determine the inhibition type of these components and corresponding inhibition constants. The results demonstrated that all the 4 compounds had no significance to inhibit the activities of CYP2E1 and CYP2C11.

Effects of two triterpenoids from seeds on insulin resistance of 3T3-L1 adipocytes.

Insulin resistance (IR) is a physiological abnormality that occurs when insulin fails to activate the signal transduction pathway in target organs. It was found that supplementation of seeds with oral antidiabetic medicines helps improve blood glucose control by enhanced β cells activity and alleviation of IR. However, the activities and related mechanisms of phytochemicals from seeds have not been thoroughly explored.

The Effect of Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics.

Proteomics and intestinal flora were used to determine the mechanism of immune modulatory effects of polysaccharide on immunosuppressed mice. The results showed that compared with the model group, polysaccharide could increase thymus and spleen indices and improve thymus tissue structure in mice; IL-2 and IL-4 contents were significantly increased and IL-6 and TNF-α contents were significantly decreased; serum acid phosphatase (ACP), lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC) activities were increased ( < 0.05); in the liver, superoxide dismutase (SOD) and catalase (CAT) activities were increased ( < 0.

Structural Characterization and Anticoagulant Activity of a 3--Methylated Heteroglycan From Fruiting Bodies of .

, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of (PPp-W) and evaluate its anticoagulant activity . The high-performance liquid chromatography and GC-MS analysis indicated that PPp-W with a molecular weight of 27.

[Effects of swimming and epigallocatechin gallate on interstitial proteins expression of myocardium from type 2 diabetic rats].

Objective: To observe the effects of swimming training and/or epigallocatechin gallate(EGCG) on the expression of myocardial interstitial protein and its relationship with transforming growth factor-beta(TGF-β)/Smads signaling pathway in type 2 diabetic rats.

Methods: Male SD rats(SPF grade, 6 weeks old) were fed with high-fat diet for 6 weeks and intraperitoneal injection of streptozotocin(STZ, 30 mg/kg). The rats were randomly divided into four groups: diabetic model group(D), diabetes EGCG group(DG), diabetes exercise group(DE), and diabetes exercise EGCG group(DEG), with 8 rats in each group, and 6 male SD rats in the same batch were added as the normal C group.