Mitochondrial protein heterogeneity stems from the stochastic nature of co-translational protein targeting in cell senescence.

A decline in mitochondrial function is a hallmark of aging and neurodegenerative diseases. It has been proposed that changes in mitochondrial morphology, including fragmentation of the tubular mitochondrial network, can lead to mitochondrial dysfunction, yet the mechanism of this loss of function is unclear. Most proteins contained within mitochondria are nuclear-encoded and must be properly targeted to the mitochondria.

Ag-modified CuO cavity arrays as a SERS-electrochemical dual signal platform for thiram detection.

Rapid and sensitive determination of pesticide residues in fruits and vegetables is critical for human health and ecosystems. This paper used an Ag-modified CuO sphere-cavity array (CuO@Ag) electrode as a thiram SERS/electrochemical dual readout detection platform. Numerous Raman "hotspots" generated by uniformly distributed silver nanoparticles, charge transfer at the CuO@Ag interface, and the formation of Ag-thiram complexes contribute to the significant enhancement of this SERS substrate, which results in excellent SERS performance with an enhancement factor up to 1.

Association of sleep disorders with asthma: a meta-analysis.

Background: Animal experiments and clinical trials have revealed a potential relationship between sleep disorders and asthma. However, the associations between these factors remain unclear.

Material And Methods: We searched PubMed, Embase, Web of Science and Cochrane Library databases for eligible studies published before 30 December 2022.

A SERS/electrochemical dual-signal readout immunosensor using highly-ordered Au/Ag bimetallic cavity array as the substrate for simultaneous detection of three β-adrenergic agonists.

A surface-enhanced Raman scattering (SERS)/electrochemical dual-signal readout immunosensor was developed for simultaneous detection of β-adrenergic agonists salbutamol (SAL), ractopamine (RAC) and phenylethanolamine A (PA). The highly-ordered gold/silver bimetallic cavity array (BMCA) was prepared by electrodepositing Au/Ag nanoparticles to the interstice of highly ordered close-packed polystyrene templates. After electrochemical and SERS characterization, the BMCA was used as the substrate for constructing SERS/electrochemical dual-signal readout immunosensor.

Selective detection of HSO in living cells using a pyrroloquinoline structure containing turn-on fluorescent probe.

A turn-on fluorescence probe PQP-1 with a pyrroloquinoline skeleton has been designed and synthesized. Probe PQP-1 showed high sensitivity to HSO, low detection limit (16.83 nM), and a wide linear range (50-3000 μM).

HO/Glucose Sensor Based on a Pyrroloquinoline Skeleton-Containing Molecule Modified Gold Cavity Array Electrode.

HO-related metabolites are essential indicators in clinical diagnosis because the accumulation of such reactive oxygen species could cause the risk of cardiovascular disease. Herein, we reported an electrochemical sensor to determine HO and glucose. The pyrroloquinoline skeleton containing molecules (PQT) were used as the electrocatalyst and the gold cavity array (GCA) electrodes as the supporting electrode.

Eliminating Stubborn Insulated Deposition by Coordination Effect to Boost Zn Electrode Reversibility in Aqueous Electrolyte.

Aqueous rechargeable zinc-ion batteries (ZIBs) have recently shined in energy storage and transmission, which are due to high safety and low cost. However, the extremely stubborn by-products in the Zn anode severely inhibited the Zn adsorption/desorption and exacerbated the dendrite formation. Herein, we report a facile strategy to eliminate inert Zn(OH)SO·xHO for the improvement of ZIBs according to the coordination effect by employing ethylenediaminetetraacetic acid-diamine (EDTA-2Na) as a coordination additive in traditional electrolyte.

Metformin in Combination with Malvidin Prevents Progression of Non-Alcoholic Fatty Liver Disease via Improving Lipid and Glucose Metabolisms, and Inhibiting Inflammation in Type 2 Diabetes Rats.

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the primary causes of chronic liver disease and is closely linked to insulin resistance, type 2 diabetes mellitus (T2DM), and dyslipidemia. However, no effective drug therapies have been approved to treat this disease. The present research aimed to evaluate the therapeutic effects of the combination of oral hypoglycemic drug metformin (MET) and a natural product malvidin (MAL) on hepatic damage in HFD/STZ-induced diabetic rats.

A close-up on COVID-19 and cardiovascular diseases.

Aims: To analyze the potential mechanism of cardiovascular dysfunctions induced by Coronavirus Disease 2019 (COVID-19) and to evaluate more effective therapeutic pathways for patients with cardiovascular diseases.

Data Synthesis: COVID-19 mainly invades the lungs, causing its serious damage. Studies found that COVID-19 induced the renin-angiotensin system imbalance, inflammatory storm, hypoxemia, stress response, and so on; all contributed to hypertension and serious myocardial damage in the process of virus pathogenesis, even increasing mortality in COVID-19 patients.

NBD-BPEA regulates Zn- or Cu-induced Aβ aggregation and cytotoxicity.

Abnormal interaction of amyloid-β peptide (Aβ) and metal ions is proved to be related to the etiology of Alzheimer's disease (AD). Using metal chelators to reverse metal-triggered Aβ aggregation has become one of the potential therapies for AD. In our work, the effect of metal chelator, NBD-BPEA, on Zn- or Cu-mediated Aβ aggregation and neurotoxicity has been systematically studied.

Electrochemical detection of carcinoembryonic antigen.

In this work, a sandwich-type electrochemical immunosensor for carcinoembryonic antigen (CEA) detection has been constructed and tested. Unlike many other sensors using external electrochemical species in the electrolyte to generate an electrochemical signal, a ferrocene derivative has been integrated into the design of the sensor to provide an internal reporting system, allowing detection of CEA in buffers and biological samples. Gold nanoparticles, which have been used to increase the conductivity of sensing surfaces, also carry immobilized secondary anti-CEA and a ferrocene derivative.

Two macrocyclic polyamines as modulators of metal-mediated Aβ40 aggregation.

Dysfunctional interactions of amyloid-β (Aβ) with Zn and Cu ions are proved to be related to the etiology of Alzheimer's disease (AD). Disruption of these metal-Aβ interactions using metal chelators holds considerable promise as a therapeutic strategy to combat this incurable disease. Herein, we report that two cyclam derivatives (L1 and L2) are capable of modulating Zn(2+)/Cu(2+)-mediated Aβ40 aggregation, reactive oxygen species (ROS) production, and neurotoxicity.

Detection of dopamine on a mercapto-terminated hexanuclear Fe(III) cluster modified gold electrode.

In this study, a novel mercapto-terminated hexanuclear iron(III) cluster [Fe6O2(OH)2(O2CC6H4SCH3)10(hep)2]·CH3CN·CH2Cl2 [hep=2-(2-hydroxyethyl) pyridine] (Fe6) modified Au electrode was fabricated, having highly sensitive dopamine (DA) detection capabilities. In such Fe6 molecules, 10 thiomethyl groups are located at the periphery of the cluster, which enable the Fe6 molecules to self-assemble onto the surface of Au electrodes through the formation of Au-S bonds. The as-prepared Fe6-modified Au electrode (Au/Fe6) exhibits excellent electrocatalytic activity for the oxidation of dopamine (DA) in PBS with a diffusion coefficient of 3.

Using a silver-enhanced microarray sandwich structure to improve SERS sensitivity for protein detection.

A simple and sensitive method, based on surface-enhanced Raman scattering (SERS), for immunoassay and label-free protein detection is reported. A series of bowl-shaped silver cavity arrays were fabricated by electrodeposition using a self-assembled polystyrene spheres template. The reflection spectra of these cavity arrays were recorded as a function of film thickness, and then correlated with SERS enhancement using sodium thiophenolate as the probe molecule.

[Fabrication of two dimensional silver cavity array and its application in SERS detection].

The highly ordered two dimensional micro-/nano-sized silver cavity was fabricated by galvanostatic multistep method using ordered arrays of close-packed 700 nm diameter polystyrene spheres as templates. The morphology and the surface plasmon resonance of this silver cavity array were characterized by scanning electron microscope and reflectance UV-Vis spectra, and surface-enhanced Raman scattering (SERS) spectra of p-aminothiophenol (PATP) and Rhodamine 6G (R6G) on as-prepared substrate were also studied. The results show that: the depth of cavities can be easily regulated through varying the parameters of the electrochemical deposition.

Hydrogen peroxide biosensor based on microperoxidase-11 immobilized in a silica cavity array electrode.

Hydrogen peroxide biosensor based on the silica cavity array modified indium-doped tin oxide (ITO) electrode was constructed. An array of silica microcavities was fabricated by electrodeposition using the assembled polystyrene particles as template. Due to the resistance gradient of the silica cavity structure, the silica cavity exhibits a confinement effect on the electrochemical reactions, making the electrode function as an array of "soft" microelectrodes.

Fabrication of a bowl-shaped silver cavity substrate for SERS-based immunoassay.

In this study, a metal sandwich substrate bridged by an immunocomplex has been created for a surface enhanced Raman scattering (SERS)-based immunoassay. The bottom bowl-shaped silver cavity thin film layer was prepared by electrodeposition using a closely packed monolayer of 700 nm diameter polystyrene spheres as a template. The reflection spectra of the films were recorded as a function of film thickness, and then correlated with SERS enhancement using p-aminothiophenol as the probe molecule.