Enhanced Expression in Silkworm Silk Gland Promotes DNA Replication and Silk Production.

Silkworm is an economically important insect that synthetizes silk proteins for silk production in silk gland, and silk gland cells undergo endoreplication during larval period. Transcription factor Myc is essential for cell growth and proliferation. Although silkworm gene has been identified previously, its biological functions in silkworm silk gland are still largely unknown.

Genome-wide identification of target genes for transcription factor BR-C in the silkworm, Bombyx mori.

Transcription factor Broad Complex (BR-C) is an ecdysone primary response gene in insects and participates in the regulation of insect growth and development. In this study, we performed a genome-wide identification of BR-C target genes in silkworm (Bombyx mori) using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). As a result, a total of 1006 BR-C ChIP peaks were identified, and 15% of peaks were located in the promoter regions of 133 protein-coding genes.

Identification of Salt Tolerance-related microRNAs and Their Targets in Maize ( L.) Using High-throughput Sequencing and Degradome Analysis.

To identify the known and novel microRNAs (miRNAs) and their targets that are involved in the response and adaptation of maize () to salt stress, miRNAs and their targets were identified by a combined analysis of the deep sequencing of small RNAs (sRNA) and degradome libraries. The identities were confirmed by a quantitative expression analysis with over 100 million raw reads of sRNA and degradome sequences. A total of 1040 previously known miRNAs were identified from four maize libraries, with 762 and 726 miRNAs derived from leaves and roots, respectively, and 448 miRNAs that were common between the leaves and roots.