Cloning and functional analysis of the gene in L.

The gene plays an important role in the formation of apical meristem and organ edges in plants. The apical meristematic tissue of () is associated with cold resistance, however, the role of the gene in cold resistance of is unclear. In this study, we used bioinformatics software to analyze the structure of gene, real-time fluorescence quantitative PCR to detect the expression level of , constructed transgenic by the flower dipping method and subcellular localization for functional validation.

Corrigendum: Transcriptomic insights into root development and overwintering transcriptional memory of L. grown in the field.

[This corrects the article DOI: 10.3389/fpls.2022.

Genome-Wide Identification of C2H2 ZFPs and Functional Analysis of under Low-Temperature Stress in Winter Rapeseed ().

Zinc-finger protein (ZFP) transcription factors are among the largest families of transcription factors in plants. They participate in various biological processes such as apoptosis, autophagy, and stemness maintenance and play important roles in regulating plant growth and development and the response to stress. To elucidate the functions of ZFP genes in the low-temperature response of winter ( L.

Discovery of anti-stroke active substances in Guhong injection based on multi-phenotypic screening of zebrafish.

Ischemic stroke is a leading cause of death worldwide, and it remains an urgent task to develop novel and alternative therapeutic strategies for the disease. We previously reported the positive effects of Guhong injection (GHI), composed of safflower extract and aceglutamide, in promoting functional recovery in ischemic stroke mice. However, the active substances and pharmacological mechanism of GHI is still elusive.

Transcriptomic Insights Into Root Development and Overwintering Transcriptional Memory of L. Grown in the Field.

As the only overwintering oil crop in the north area of China, living through winter is the primary feature of winter rapeseed. Roots are the only survival organ during prolonged cold exposure during winter to guarantee flowering in spring. However, little is known about its root development and overwintering memory mechanism.

A Chromosome Level Genome Assembly of a Winter Turnip Rape (.) to Explore the Genetic Basis of Cold Tolerance.

Winter rapeseed (.) is an important overwintering oilseed crop that is widely planted in northwest China and suffers chronic low temperatures in winter. So the cold stress becomes one of the major constraints that limit its production.

Guhong injection promotes post-stroke functional recovery via attenuating cortical inflammation and apoptosis in subacute stage of ischemic stroke.

Background: As a leading cause of death and disability, alternative therapies for stroke are still limited by its complicated pathophysiological manifestations. Guhong injection (GHI), consisting of safflower aqueous extract and aceglutamide, has been widely applied for the clinical treatment of cerebrovascular diseases, especially ischemic stroke and post-stroke recovery, in China. Recently, a series of studies have reported the positive effect of GHI against cerebral ischemia/reperfusion injury via targeting various molecular mechanisms.

iTRAQ-based quantitative proteome analysis insights into cold stress of Winter Rapeseed (Brassica rapa L.) grown in the field.

Winter rapeseed (Brassica rapa L.) is a major oilseed crop in Northern China, where its production was severely affected by chilling and freezing stress. However, not much is known about the role of differentially accumulated proteins (DAPs) during the chilling and freezing stress.

Identification of differentially expressed genes involved in amino acid and lipid accumulation of winter turnip rape (Brassica rapa L.) in response to cold stress.

Winter turnip rape (Brassica rapa L.) is an important overwintering oil crop that is widely planted in northwestern China. It considered to be a good genetic resource for cold-tolerant research because its roots can survive harsh winter conditions.

Screening and verification of reference genes for analysis of gene expression in winter rapeseed (Brassica rapa L.) under abiotic stress.

Winter rapeseed (Brassica rapa L.) is the main oilseed crop in northern China and can safely overwinter at 35 (i.e.

Ecological and economic benefits of planting winter rapeseed (Brassica rapa L.) in the wind erosion area of northern China.

Winter and early spring wind soil erosion have considerable impacts on ecosystems, human well-being and agricultural production in the low precipitation zones of northern China. Little is known about the impact of growing winter rapeseed on ecological cropping systems and the associated economic benefits in the wind erosion area. To explore the winter rapeseed cover effect, we conducted a field experiment in which we covered the soil with winter rapeseed, winter wheat and wheat stubble at different plant density levels and used the spring bare ground as the control (CK).

[Safe wintering and economic and ecological benefit of winter rapeseed in dry and cold areas of northern China].

The purpose of this study was to realize the security of safe wintering of winter rapeseed in dry and cold regions of northern China. Experiments were conducted with 18 winter rapeseed (Brassica campestris) varieties at 57 sites from 2008 to 2013 to statistically analyze the wintering rate variation of different varieties in dry and cold regions of northern China. The results showed that, the wintering rate varied from 70% to 90% during the study period in different regions, which had no significant difference between different years and varieties, and had high stability and remarkable economic benefit.

Noncovalent assembly of reduced graphene oxide and alkyl-grafted mesoporous silica: an effective drug carrier for near-infrared light-responsive controlled drug release.

In this paper, we report the assembly of reduced graphene oxide (RGO) and mesoporous silica grafted with alkyl chains (MSN-C) to develop a new class of drug carriers which are able to deliver the loaded drug molecules into living cells upon exposure to near-infrared (NIR) light. This novel drug carrier consists of a structure formed by the noncovalent interaction of RGO caps and alkyl chains on the surface of MSN-C. The capping of RGO sheets on mesoporous silica effectively blocks the pore mouths in the absence of NIR light.

Redox-responsive degradable honeycomb manganese oxide nanostructures as effective nanocarriers for intracellular glutathione-triggered drug release.

Redox-responsive degradable honeycomb manganese oxide (hMnO2) nanostructures consisting of some lamellar MnO2 platelets were established as a new class of drug carriers for intracellular glutathione-triggered drug release.

Nanometer-sized manganese oxide-quenched fluorescent oligonucleotides: an effective sensing platform for probing biomolecular interactions.

We propose a novel template-assisted strategy to prepare nanometer-sized manganese oxide (nano-MnO2) by self-assembly of some MnO2 platelets and demonstrate its application as a new class of biosensing platform for probing DNA hybridization and aptamer-target interaction in a homogeneous solution.

Remote-controlled drug release from graphene oxide-capped mesoporous silica to cancer cells by photoinduced pH-jump activation.

Remote light control of drug release enhances our ability to address the complexity of biological systems because of its remarkable spatial/temporal resolution. Here, a new class of remote-controlled release system by incorporating photoacid generator (PAG) into graphene oxide-capped mesoporous silica was designed for delivering drug payloads to cancer cells via photoinduced pH-jump activation. PAG was immobilized on pore wall of the boronic acid-grafted mesoporous silica via strong physical adsorption, and then the nanoparticle was capped with graphene oxide sheet by an acid-labile boroester bond, leading to the formation of nanogated ensemble (MSP-BA-GO).

[Effects of antisense acid invertase gene on reducing sugar and starch contents of potato (Solanum tuberosum L.) tuber stored at low temperature].

Two transgenic potato lines with antisense AcInv gene and the non-transgenic varieties were used to test the reducing sugar and starch contents. The results indicated that the reducing sugar content increased and total starch content decreased in all samples after tubers were stored at 4 degrees C for 40 d. The reducing sugar content in tubers of transgenic line "Anti-AcInv Atlantic" and "Anti-AcInv Gannongshu No.