Introduction: , a pivotal salt-tolerant plant species in Central Asian salt desert ecosystems, has garnered significant attention due to its resilience under harsh environmental conditions. This study investigates the response mechanisms of melatonin on the dynamic physiology and transcriptomics of , a critical salt-tolerant plant species in Central Asian salt desert ecosystems. Despite significant progress in understanding plant salt tolerance, research on the positive effects of melatonin on , particularly its impact on seed germination and the underlying physiological and molecular mechanisms, remains limited.
View Article and Find Full Text PDFFruit preservation materials play an instrumental role in preventing fruit deterioration and extending shelf life. However, existing fresh-keeping materials often prove inadequate in simultaneously achieving antibacterial properties, maintaining freshness, antioxidant effects, good biocompatibility, and prolonged fruit shelf life. Therefore, we present the first preparation of a natural polysaccharide spray hydrogel (Q/O/Zn hydrogel), loaded with chlorogenic acid‑zinc nanoparticles (CA@ZnNPs), utilizing quaternary ammonium insect chitosan (QECS) and oxidized pullulan (OPUL) for the preservation of perishable fruits.
View Article and Find Full Text PDFIntroduction: Mitochondrial DNA (mtDNA) has been extensively utilized in archeology, human evolutionary genetics, and forensic genetic for over three decades, primarily due to its maternal inheritance and relatively high mutation rate. The Chinese Han, the largest and most widely distributed ethnic group in China, have been the focus of numerous genetic studies. However, the forensic parameters and genetic structure of the Shandong Han, specifically in relation to the whole mitochondrial genome, remain undocumented.
View Article and Find Full Text PDFInfected wounds caused by bacteria commonly cause delayed wound healing and even serious tissue damage, evolving into one of the most problematic clinical issues. Traditional therapies and wound dressings cannot achieve either the ideal antibiotic activity or the accelerated wound healing outcomes. Based on our previous finding that glycoproteins (PAGP) derived from medicinal animal insect Periplaneta americana L.
View Article and Find Full Text PDFIdentifying the species and origin of adhesives in criminal investigations aids in narrowing inquiry scope and supporting case detection. This study introduces two advanced combined analytical techniques for distinguishing adhesive species, including attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) combined with Raman spectroscopy, and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) together with multivariate statistical analysis. ATR-FTIR categorized seven adhesives into three groups based on the base materials, with further differentiation achieved via Raman spectra.
View Article and Find Full Text PDFChironomidae is a cosmopolitan and species-rich family of insects, with many species serving as useful indicators of aquatic ecosystem health. In this study, we newly sequenced six species of Goetghebuer, 1922 (Chironomidae: Chironominae) by high-throughput sequencing technology. We analyzed characters of the mitochondrial genome, including the sequence length, nucleotide composition, and evolutionary rates of this genus.
View Article and Find Full Text PDFTo investigate the synergistic effect of IAA and melatonin (MT) on three plants to alleviate the effects of salt damage on plants, we aim to determine the optimal concentrations of exogenous hormone treatments that improve salinity resistance for each species. In this experiment, three desert plants, , , and , which are common in Wuhai City, were used as plant materials. Two time periods (12 h,24 h) of exogenous hormone IAA (100 μmol/L) and exogenous melatonin concentration (0, 100, 200, 300 μmol/L) were used to treat the three desert plants in saline soil under different conditions of exogenous IAA and exogenous melatonin.
View Article and Find Full Text PDFChitosan samples were prepared from the shells of marine animals (crab and shrimp) and the cell walls of fungi (agaricus bisporus and aspergillus niger). Fourier-transform infrared spectroscopy (FT-IR) was used to detect their molecular structures, while headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was employed to analyze their odor composition. A total of 220 volatile organic compounds (VOCs), including esters, ketones, aldehydes, etc.
View Article and Find Full Text PDFBackground: Amauroderma rugosum (AR) is a medicinal mushroom commonly used to treat inflammation, gastric disorders, epilepsy, and cancers due to its remarkable anti-inflammatory and anti-oxidative properties. This study was designed to evaluate the pharmacological effects of AR and its underlying mechanism of action against ulcerative colitis (UC) in vitro and in vivo.
Methods: A UC mouse model was established by administration of dextran sulfate sodium (DSS).
The goal of the following study is to clarify whether the skeletal remains over 70 years old from missing persons and their alleged relatives shared identical Y-STR loci. Nowadays, advances in ancient DNA extraction techniques and approaches of using multiple different Y-STRs have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Given the ages and conditions of the skeletal remains, ancient DNA extraction methods can be used to maximize the probability of DNA recovery.
View Article and Find Full Text PDFMethicillin-resistant Staphylococcus aureus (MRSA) and other drug-resistant bacteria have become more common in recent years, which has made it extremely difficult to treat and heal many different kinds of wounds and caused enormous financial losses. Because of its unique "Trojan horse" function, Ga has been recognized as a new possible candidate for inhibiting and eradicating drug-resistant bacteria. Furthermore, natural polysaccharide materials with outstanding biological characteristics, such as insect chitosan (CS) and pullulan (PUL), have attracted significant interest.
View Article and Find Full Text PDFUlcerative colitis (UC) is a chronic disease with diffuse mucosal inflammation limited to the colon. A topical drug delivery system that could be facilely performed and efficiently retained at colon are attractive for clinical ulcerative colitis treatment. Herein, a novel platform for rectal administration of thermosensitive hydrogel co-loaded with nanoparticles to treat ulcerative colitis was developed.
View Article and Find Full Text PDFThe rising crime rate associated with document forgery has a significant impact on public safety and social stability. In document fraud cases, determining the origin of a particular stamp-pad ink is the most important objective. In this study, a comprehensive analysis of the volatile compounds in quick-drying stamp-pad inks from six commonly used brands were performed for the first time, utilizing a combination of headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) and multivariate statistical analysis methods.
View Article and Find Full Text PDFThe auxin/indole-3-acetic acid () and auxin response factor () genes are two crucial gene families in the plant auxin signaling pathway. Nonetheless, there is limited knowledge regarding the and gene families in . In this study, we first identified 33 putative and 35 in the genome.
View Article and Find Full Text PDFLocal recurrence and distant metastasis are the main causes of death in patients with cancer. Only considering species abundance changes when identifying markers of recurrence and metastasis in patients hinders finding solutions. Consideration of microbial abundance changes and microbial interactions facilitates the identification of microbial markers of tumour recurrence and metastasis.
View Article and Find Full Text PDFWound-healing of drug-resistant bacterial infections has always been a clinical challenge. The design and development of effective and economically safe wound dressings with antimicrobial activity and healing-promoting properties is highly desirable, especially in the context of wound-infections. Herein, we designed a physical dual-network multifunctional hydrogel adhesive based on polysaccharide material for the treatment of full-thickness skin defects infected with multidrug-resistant bacteria.
View Article and Find Full Text PDFMitochondrial genomics, as a useful marker for phylogenetics and systematics of organisms, are important for molecular biology studies. The phylogenetic relationships of the generic complex remains controversial, due to lack taxonomy and molecular information. In this study, we newly sequenced mitogenomes of 14 species of the generic complex.
View Article and Find Full Text PDFNon-small-cell lung cancer (NSCLC) is the subtype of lung cancer, which accounts for about 85% of diagnosed lung cancer cases, and is without any effective therapy. Emerging evidence has revealed microRNA-598 (miR-598) as potential therapeutic target and diagnostic marker of NSCLC. In the present study, we sought to define the role of mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) containing miR-598 in NSCLC.
View Article and Find Full Text PDFPeriplaneta americana has been used medicinally for years to treat a wide variety of skin lesions or ulcers. However, a sizable portion of the drug residues that are retained after extraction are routinely thrown away, thus posing a hazard to the environment and depleting resources. In this study, low molecular weight Periplaneta americana chitosan (LPCS) and high molecular weight Periplaneta americana chitosan (HPCS) were extracted from Periplaneta americana residue (PAR) based on the conventional acid-base method and two deacetylation methods.
View Article and Find Full Text PDFDue to its capabilities for wound healing, antimicrobial defense, hemostasis, and biodegradation, chitosan has seen increased use in biomedical disciplines in recent years. In the meantime, efforts have been made to develop and use insect chitosan as a source to address the seasonal, irritating, and regional shortcomings of traditional shrimp and crab chitosan. In this study, a new type of insect chitosan (DCS) was first extracted from Eupolyphaga sinensis Walker by a low-temperature intermittent method and was compared with commercially available pharmaceutical chitosan (CS).
View Article and Find Full Text PDF. (also called as longan) is a subtropical and tropical evergreen tree belonging to the Sapindaceae family and is widely distributed in China, Southeast Asia and South Asia. The pulp of longan fruit is a time-honored traditional medicinal and edible raw material in China and some Asian countries.
View Article and Find Full Text PDFSpecies identification of unknown biological samples is crucial for forensic applications, especially in cases of explosion, disaster accidents, and body mutilation after murdering, as well as poaching, illegal trade in endangered animals, and meat food fraud. In this study, we identified 60 volatile organic compounds (VOCs) in fresh skeletal muscle tissues of seven different animal species (cattle, sheep, pigs, rabbits, rats, chickens and carp) and a human dead body by headspace-gas-chromatography ion-mobility spectrometry (HS-GC-IMS), and compared their differences by retention time, drift time and molecular weight. The results showed that these VOCs formed different gallery plot fingerprints in the skeletal muscle tissues of the human dead body and seven animal species.
View Article and Find Full Text PDFNatural polysaccharides are critical to a wide range of fields (e.g., medicine, food production, and cosmetics) for their various remarkable physical properties and biological activities.
View Article and Find Full Text PDFCarnitine-acylcarnitine translocase deficiency (CACTD) is a rare and life-threatening autosomal recessive disorder of fatty acid β-oxidation (FAO). Most patients with CACTD develop severe metabolic decompensation which deteriorates progressively and rapidly, causing death in infancy or childhood. As CACTD in some patients is asymptomatic or only with some nonspecific symptoms, the diagnosis is easy to be ignored, resulting in sudden death, which often triggers medical disputes.
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