Upregulation of OGT-mediated EZH2 O-GlcNAcylation Promotes Human Umbilical Vein Endothelial Cell Proliferation, Invasion, Migration, and Tube Formation in Gestational Diabetes Mellitus.

O-linked N-acetylglucosamine transferase (OGT)-catalyzed O-linked N-acetylglucosamine glycosylation (O-GlcNAcylation) is closely associated with diabetes progression. This study aims to investigate the mechanism of OGT in regulating endothelial dysfunction in gestational diabetes mellitus (GDM). Expressions of OGT, O-linked N-acetylglucosamine (O-GlcNAc), enhancer of zeste homolog 2 (EZH2), and HEK27me3 in human umbilical vein endothelial cells (HUVECs) and GDM-derived HUVECs (GDM-HUVECs) were assessed by western blot.

Gestational diabetes-combined excess weight gain exacerbates gut microbiota dysbiosis in newborns, associated with reduced abundance of , , and .

Background: Existing literature indicates that Gestational diabetes mellitus (GDM) and maternal obesity disrupt the normal colonization of the neonatal gut microbiota alone. Still, the combined impact of GDM and excessive gestational weight gain (EGWG) on this process remains under explored. The association between gestational weight gain before/after GDM diagnosis and neonatal gut microbiota characteristics is also unclear.

Investigation of a hemophilia family with one female hemophilia A patient and 12 male hemophilia A patients.

Hemophilia A (HA) is an X-chromosome-linked recessive genetic disorder. Female carriers may have bleeding symptoms, but rarely have moderate or severe disease. We identified a female patient with moderate HA by pedigree tracking and genetic testing in a HA family involving consanguineous marriage.

Levofloxacin-loaded silicone contact lenses materials for ocular drug delivery.

Silicone contact lenses (SCL), as an emerging ocular drug delivery system, achieve controlled drug release. However, the existing drug loading methods have limitations such as low drug uptake, complicated operation process, poor welling rate and transmittance of the lens after drug loading. In this study, an effective microemulsion soaking method was proposed to increase the drug-loading capacity of silicone contact lenses.

Disentangled global and local features of multi-source data variational autoencoder: An interpretable model for diagnosing IgAN via multi-source Raman spectral fusion techniques.

A single Raman spectrum reflects limited molecular information. Effective fusion of the Raman spectra of serum and urine source domains helps to obtain richer feature information. However, most of the current studies on immunoglobulin A nephropathy (IgAN) based on Raman spectroscopy are based on small sample data and low signal-to-noise ratio.

Fucoidan treats chemotherapy-induced alopecia and helps cyclophosphamide treat tumors.

Chemotherapy-induced alopecia (CIA) represents one of the most common side effects of cancer treatment. Currently, scalp cooling systems are utilized to treat CIA, but their safety and effectiveness remain limited. The objective of this study was to investigate the effect of fucoidan on CIA and to elucidate the possible mechanism of fucoidan in treating CIA.

Efficient Selection of the 2,4-Dichlorophenoxyacetic Acid Nanobody Gene from the Phage Library Constructed with Sorted Specific Cells and Expression in Plants to Confer Herbicide Resistance.

Immunomodulation in biotechnological processes requires an adequate level of specific, high-affinity recombinant antibodies expressed in the affected cells. Here, we report a new strategy to obtain a sensitive nanobody against the 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide. Unlike the previous methods that used total peripheral blood lymphocytes, specific peripheral lymphocytes stained with a fluorescein isothiocyanate-labeled 2,4-D coating antigen were isolated via fluorescence-activated cell sorting and used for phage display library construction.

Effectiveness, safety, and cost-effectiveness of norvancomycin in the management of acute hematogenous osteomyelitis in pediatric patients: A retrospective case study.

This study was designed to investigate the effectiveness, safety, and cost-effectiveness of norvancomycin in the management of acute hematogenous osteomyelitis in pediatric patients. We conducted a retrospective study on cases of osteomyelitis in pediatric patients treated with norvancomycin or vancomycin at Hebei Children's Hospital from January 2015 to February 2023. The patients were categorized into the norvancomycin group and the vancomycin group.

Graph attention automatic encoder based on contrastive learning for domain recognition of spatial transcriptomics.

Spatial transcriptomics is an emerging technology that enables the profiling of gene expression in tissues while preserving spatial location information. This innovative approach is anticipated to provide a comprehensive understanding of the spatial distribution of different cells within tissues and facilitate in-depth analysis of tissue structure. To accurately recognize spatial domains from spatial transcriptomics, we have introduced a generalized deep learning method called GAAEST (Graph Attention-based Autoencoder for Spatial Transcriptomics).

Anaerobic oxidation of methane coupled to reductive immobilization of hexavalent chromium by "Candidatus Methanoperedens".

The anaerobic oxidation of methane (AOM) carried out by anaerobic methanotrophic archaea (ANME) plays an important role in mitigating methane emissions from aqueous environments and has applications in bioremediation and wastewater treatment. Previous studies showed that AOM could be coupled to chromate reduction. However, the specific responsible microorganisms and the biochemical mechanisms are unclear.

Chitosan quaternary ammonium salt-oxidized sodium alginate-glycerol-calcium ion biobased self-healing hydrogels with excellent spontaneous repair performance.

Self-healing hydrogels have attracted wide attention because of their potential applications in various fields. However, the complex processes, environmental requirements, and insufficient functionality limit their practical application. Herein, we synthesized a chitosan quaternary ammonium salt-oxidized sodium alginate-glycerol-calcium ion (HACC-OSA-Gly-Ca) biobased hydrogel with a multi-network structure that exhibits excellent self-healing abilities.

Acute Hematogenous Osteomyelitis in Pediatric Patients.

This article focuses on the advancements made in diagnostic techniques and drug interventions of acute hematogenous osteomyelitis. A diagnosis necessitates a combination of factors, including inflammatory markers and imaging findings, as well as the collection of specimens for culture when feasible. Subsequently, treatment should be based on epidemiology, mechanisms of resistance, and susceptibility findings.

3D Printed Leaf-Vein-Like AlO/EP Biohybrid Structures with Enhanced Thermal Conductivity.

The high computility of electronic components put urgent requirements on the dissipation efficiency of a high thermal conductive substrate. Herein, inspired by the nature structure, leaf-vein-like AlO skeleton was first designed though topology optimization algorithm and manufactured via vat photopolymerization (VPP) 3D printing, then compounded with epoxy (EP) to prepare leaf-vein-like biohybrid structures. The biohybrid structure had a high λ (14.

Biodegradable packaging paper derived from chitosan-based composite barrier coating for agricultural products preservation.

Development of green packaging materials is essential to replace traditional plastics in fresh agricultural products preservation. Herein, a coated paper was designed by applying chitosan-based composite coating on paper substrate through a facile automatic coating method. The hydroxypropyl trimethyl ammonium chloride chitosan (HACC) was obtained by direct quaternization via the introduction of hydroxypropyl trimethyl ammonium chloride into the amino group of chitosan, then mixed with polyvinyl alcohol (PVA) to prepare the HACC/PVA coating.

Item response theory analysis of the Chinese version compulsive shopping scale.

Compulsive shopping, a behavioral disorder with significant personal and social repercussions, necessitates reliable assessment tools, especially within different cultural contexts. While several scales exist to measure compulsive buying behavior, there is a lack of validated instruments tailored to the Chinese population. This study aimed to examine the psychometric properties of the Chinese version of the Compulsive Shopping Scale (CSS) using Item Response Theory (IRT).

The integration of psychology and medicine: an empirical study of curriculum reform from the perspective of China.

Under the background of new medicine, innovative reform of medical education is mushrooming in Mainland, China. New medicine advocates an innovative training mode supported by medicine + X discipline. In the present study, we made use of the advantages of medical colleges to reform the curriculum of applied psychology and constructed an innovative curriculum system by integrating medicine with psychology.

Sulfated polysaccharide from Apostichopus japonicus viscera exhibits anti-inflammatory properties in vitro and in vivo.

Polysaccharides from sea cucumbers are known for their biological activities, but little is known about those from sea cucumber viscera. The present study isolated a sulfated polysaccharide (SCVP-2) from the viscera of Apostichopus japonicas, which had a molecular weight of 209.1 kDa.

Rational Designing Microenvironment of Gas-Diffusion Electrodes via Microgel-Augmented CO Availability for High-Rate and Selective CO Electroreduction to Ethylene.

Efficient electrochemical CO reduction reaction (CORR) requires advanced gas-diffusion electrodes (GDEs) with tunned microenvironment to overcome low CO availability in the vicinity of catalyst layer. Herein, for the first time, pyridine-containing microgels-augmented CO availability is presented in CuO-based GDE for high-rate CO reduction to ethylene, owing to the presence of CO-phil microgels with amine moieties. Microgels as three-dimensional polymer networks act as CO micro-reservoirs to engineer the GDE microenvironment and boost local CO availability.

Radiomics analysis of gadoxetic acid-enhanced MRI for evaluating vessels encapsulating tumour clusters in hepatocellular carcinoma.

Purpose: The aim of this study was to develop an integrated model that combines clinical-radiologic and radiomics features based on gadoxetic acid-enhanced MRI for preoperative evaluating of vessels encapsulating tumour clusters (VETC) patterns in hepatocellular carcinoma (HCC).

Methods: This retrospective study encompassed 234 patients who underwent surgical resection. Among them, 101 patients exhibited VETC-positive HCC, while 133 patients displayed VETC-negative HCC.

Gadoxetic Acid-Enhanced MRI-Based Radiomic Models for Preoperative Risk Prediction and Prognostic Assessment of Proliferative HCC.

Rationale And Objectives: Proliferative hepatocellular carcinoma (HCC) is associated with high invasiveness and poor prognosis. This study aimed to investigate the preoperative risk prediction and prognostic value of different radiomics models and a nomogram for proliferative HCC.

Materials And Methods: Patients were randomly divided into a training cohort (n = 156) and a validation cohort (n = 66) in a 7:3 ratio.

The Discovery of Highly Efficient and Promising ABA Receptor Antagonists for Agricultural Applications Based on APAn Modification.

Abscisic acid (ABA) is one of the many naturally occurring phytohormones widely found in plants. This study focused on refining APAn, a series of previously developed agonism/antagonism switching probes. Twelve novel APAn analogues were synthesized by introducing varied branched or oxygen-containing chains at the C-6' position, and these were screened.