[Identification and Nitrogen Removal Characteristics of a Heterotrophic Nitrification-Aerobic Denitrification Strain Isolated from Marine Environment].

A heterotrophic nitrification-aerobic denitrification strain named y5 was isolated from marine environment by traditional microbial isolation method using seawater as medium. It was identified as Klebsiella sp. based on the morphological, physiological and 16S rRNA sequence analysis.

[Isolation, Identification and Nitrogen Removal Characteristics of a Heterotrophic Nitrification-Aerobic Denitrification Strain y3 Isolated from Marine Environment].

A heterotrophic nitrification--aerobic denitrification bacterium named y3 was isolated from the sludge of Jiaozhou Bay using the enrichment medium with seawater as the matrix. It was identified as Pseudomonas sp. based on the morphological observation, physiological experiments and sequence analysis of 16S rRNA.

Carboxylicivirga linearis sp. nov., isolated from a sea cucumber culture pond.

A yellow-pigmented, Gram-stain-negative and facultatively anaerobic bacterium, designated FB218T, was isolated from a sediment sample collected from a sea cucumber culture pond in Rongcheng, China (36° 54′ 36″ N 122° 14′ 34″ E). Cells of strain FB218T were slender, gliding, catalase-positive and oxidase-negative. Optimal growth occurred at 30 °C, pH 6.

Colwellia arctica sp. nov., isolated from Arctic marine sediment.

Strain 435(T), a catalase- and oxidase-positive, beige-pigmented, facultatively anaerobic and Gram-stain-negative marine bacterium, was isolated from marine sediment collected in the Arctic (8°21'629''E 72°8'827''N). The cells of the type strain are short- to curve-rods and able to grow at 4-25 °C, pH 6.0-9.

Flaviflexus huanghaiensis gen. nov., sp. nov., an actinobacterium of the family Actinomycetaceae.

Strain H5(T) was isolated from a sediment sample collected from the coastal area of Qingdao, China. The cells were Gram-stain-positive, non-motile, straight or curved rods. The temperature range for growth was 20-37 °C and the pH for growth ranged from 6.

Preoperative inhalation of milrinone attenuates inflammation in patients undergoing cardiac surgery with cardiopulmonary bypass.

Objectives: The purpose of this study was to evaluate the effect of preoperative inhalation of milrinone on cardiopulmonary bypass (CPB)-related inflammation.

Subjects And Methods: A total of 30 patients undergoing cardiac surgery were recruited and randomized for preoperative inhalation of milrinone (Mil group) or normal saline (NS group), respectively. Each group had 15 patients.

Effects of co-expression of molecular chaperones on heterologous soluble expression of the cold-active lipase Lip-948.

The cold-active lipase gene Lip-948, cloned from Antarctic psychrotrophic bacterium Psychrobacter sp. G, was ligated into plasmid pColdI. The recombinant plasmid pColdI+Lip-948 was then transformed into Escherichia coli BL21.

[Effect of cardiopulmonary bypass on the expression of survivin in neutrophils].

Objective: To investigate the effects of cardiopulmonary bypass on neutrophils apoptosis and the expression of survivin.

Methods: Ten patients who scheduled for cardiac surgery under cardiopulmonary bypass were recruited as study group and 10 healthy volunteers as control. Blood samples were obtained before operation, at the end of surgery, and at 24 hours postoperatively.

Molecular cloning and tissue expression patterns of a small conductance calcium-activated potassium channel gene in turbot (Scophthalmus maximus L.).

Calcium-activated potassium channels on plasma membrane enable potassium influx into the cell with ensuing changes in plasma membrane potential and consequent effects on cellular metabolic functions. Recently, this potassium channel was reported to regulate the cellular responses of mammalian immune cells. We have postulated the presence of such a channel in fish immune cells and its potential role in immunoregulation in fish.

[Effect of spectra on growth of chlorella and isochrysis].

Focusing on the speed and efficiency, the effects of different spectra on the growth of chlorella vulgaris and isochrysis galbana Parke 8701 were investigated by using monochromatic LED (light-emitting diode) and fluorescent lamp as light sources. It was concluded that continuous spectra accelerate the top-growth-rate, blue light has the best efficiency, and the combination of them can obtain a good balance of speed and efficiency. For the purpose of measuring spectra as a parameter of irradiation quantitatively, spectra-absorbability-coefficient defined as the quanta-absorbability- efficiency of spectra for algae was calculated by means of absorption spectra of algae and emission spectra of light sources.