[Autophagy inhibitor 3-MA decreases the production and release of infectious enterovirus 71 particles].

Objective: To determine whether or not enterovirus 71 (enteroviurs 71, EV71) may induce autophagy and affect the production and release of EV71 after the treatment of autophagy inhibitor.

Methods: Western blots were performed to examine the conversion of LC3-I to LC3- II and the degradation of P62 after the RD-A cells were infected with EV71. CCID50 was determined by checking the virus titer in the supernatant of cells that treated with autophagy inhibitor 3-MA.

A novel strategy to screen Bacillus Calmette-Guérin protein antigen recognized by γδ TCR.

Background: Phosphoantigen was originally identified as the main γδ TCR-recognized antigen that could activate γδ T cells to promote immune protection against mycobacterial infection. However, new evidence shows that the γδ T cells activated by phosphoantigen can only provide partial immune protection against mycobacterial infection. In contrast, whole lysates of Mycobacterium could activate immune protection more potently, implying that other γδ TCR-recognized antigens that elicit protective immune responses.

[Expression and biological function of ULBP4 in Rosetta-gamiTMB(DE3) and mAb preparation].

Aim: To express human ULBP4 in Rosetta-gami(TM) B(DE3) and to prepare monoclonal antibody against ULBP4 for the research of gamma deltaT cells recognition mechanism.

Methods: DNA fragments of ULBP4 were derived from HO-8910 RNA by reverse-transcriptase polymerase chain reaction(RT-PCR). The fragments encoding the former 225 amino acids of ULBP4 were cloned into Histag fusion protein expression vector pET22b(+).

[Inhibition of intracellular Ca(2+)-chelating agent on Hela cell apoptosis induced by HSV-1].

Aim: To explore the variation of intracellular free-Ca (2+) concentration during Hela cell apoptosis induced by HSV-1 and inhibition of Ca(2+)-chelating agent on the apoptosis.

Methods: Apoptosis of the Hela cells infected by HSV-1 was observed under scanning electron microscope (SEM) and transmission electron microscope (TEM), and the variation of intracellular free-Ca (2+) concentration, labeled with fluorescent probe was observed under fluorescence-microscope at a series of timepoints.

Results: The Hela cells infected by HSV-1 showed typical apoptotic morphological changes.