Halomontanus rarus gen. nov., sp. nov., a novel halophilic archaeon of the family Natrialbaceae from salt lakes on the Qinghai-Xizang Plateau.

Two halophilic archaeal strains TS33 and KZCA124 were isolated from two distant salt lakes on the Qinghai-Xizang Plateau, respectively. Culture-independent analysis indicated that these two strains were original inhabitants but low abundant taxa in respective salt lakes. Strains TS33 and KZCA124 were able to grow at 20-60 °C (optimum were 42 and 35 °C, respectively), with 0.

sp. nov. and sp. nov., isolated from salt lake and saline soil.

Two halophilic archaeal strains, Gai3-2 and NJ-3-1, were isolated from salt lake and saline soil samples, respectively, collected in PR China. The 16S rRNA gene sequences of the two strains were 97.5% similar to each other.

sp. nov. and sp. nov., isolated from saline soil and a salt mine.

A polyphasic study was undertaken to determine the taxonomic position of two halophilic archaeal strains, TH32 and YPL4, isolated from saline soil and a salt mine in PR China, respectively. Strains TH32 and YPL4 both have two dissimilar 16S rRNA genes. The two strains exhibited sequence similarities of 91.

Biochemical characterization of a low salt-adapted extracellular protease from the extremely halophilic archaeon Halococcus salifodinae.

Extracellular proteases from haloarchaea can expand the application fields of proteases. Exploring novel robust proteases is of great importance. An extracellular protease HlyA from Halococcus salifodinae was obtained by heterologous expression, affinity chromatography, in vitro refolding and gel filtration chromatography.

sp. nov. and sp. nov., two novel halophilic archaea.

Two halophilic archaeal strains, C90 and YPL13, were isolated from a salt lake and a salt mine in PR China. The two strains were found to form two clusters (97.5 and 89.

[Gene therapy for tongue carcinoma mediated by oral administration of attenuated Salmonella harboring tip30 and IFN-γ genes in nude mouse model].

Purpose: To observe the effect of gene therapy mediated by oral administration of attenuated Salmonella carrying tip30 and IFN-γ genes in human tongue carcinoma nude mouse model.

Methods: 25 four-week-old BALB/C male nude mice were divided randomly into 5 groups based on the differently harboring genes in the recombinant attenuated Salmonella typhimuriums SL7207, which included blank group (PBS), control group (SL7207), IFN treatments group (SL7207-pCI-IFN), tip30 treatment group (SL7207-pCI-tip30) and the combination of IFN and tip30 treatment group (SL7207-pCI-tip30/IFN). On 10d after submandibular subcutaneous injection of Tca8113 cells into the mice, the recombinant attenuated Salmonella typhimuriums were orally administrated 3 times at 1 week interval.

[Induction of apoptosis and related genes by five kinds of dental materials on L929 cell].

Objective: To evaluate a new type of diatomite-based machinable ceramic biocompatibility by studying its induced apoptosis on L929 cell in contrasted with other prosthodontics materials.

Methods: Cell line was treated with extracting liquid containing different concentrations of diatomite-based machinable ceramic and other materials. Flow cytometry tested cell cycle progression and induced cell apoptosis.