sp. nov., isolated from rhizosphere soil of .

A psychrotolerant actinobacterium, designated strain J5903, was isolated from an alkaline soil sample from the rhizosphere of collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China. Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci. Strain J5903 grew optimally at 20‒25 °C, at pH 7.

sp. nov., isolated from a saline-alkaline soil.

A novel moderately halophilic, filamentous actinobacterium, designated as XMNu-373, was isolated from a saline-alkaline soil sample collected from the Mongolia Plateau, Dongwu County, Inner Mongolia Autonomous Region, PR China. The isolate grew optimally at 28‒37 °C, pH 7.0‒8.

Mutation analysis of gene in two Chinese families with congenital ectopia lentis in northern China.

Aim: To summarize the phenotypes and identify the underlying genetic cause of the () gene responsible for congenital ectopia lentis (EL) in two Chinese families in northern China.

Methods: A detailed family history and clinical data from all participants were collected by clinical examination. The candidate genes were captured and sequenced by targeted next-generation sequencing, and the results were confirmed by Sanger sequencing.

Blockade of the forward Na /Ca exchanger suppresses the growth of glioblastoma cells through Ca -mediated cell death.

Background And Purpose: The Na /Ca exchanger (NCX) working in either forward or reverse mode participates in maintaining intracellular Ca ([Ca ] ) homeostasis, which is essential for determining cell fate. Previously, numerous blockers targeting reverse or forward NCX have been developed and studied in ischaemic tissue injury but barely examined in glioblastoma for the purpose of anti-tumour therapy. We assessed the effect of NCX blockers on glioblastoma growth and whether NCX can become a therapeutic target.

Neuronal and Endothelial Nitric Oxide Synthases in the Paraventricular Nucleus Modulate Sympathetic Overdrive in Insulin-Resistant Rats.

A central mechanism participates in sympathetic overdrive during insulin resistance (IR). Nitric oxide synthase (NOS) and nitric oxide (NO) modulate sympathetic nerve activity (SNA) in the paraventricular nucleus (PVN), which influences the autonomic regulation of cardiovascular responses. The aim of this study was to explore whether the NO system in the PVN is involved in the modulation of SNA in fructose-induced IR rats.

Activation of muscarinic receptors protects against retinal neurons damage and optic nerve degeneration in vitro and in vivo models.

Aims: Muscarinic acetylcholine receptor agonist pilocarpine reduces intraocular pressure (IOP) of glaucoma mainly by stimulating ciliary muscle contraction and then increasing aqueous outflow. It is of our great interest to know whether pilocarpine has the additional properties of retinal neuroprotection independent of IOP lowering in vitro and in vivo models.

Methods: In rat primary retinal cultures, cell viability was measured using an MTT assay and the trypan blue exclusion method, respectively.

Establishment and application of hepatitis B virus persistent replication model in IFNAR(-/-) mouse.

The type I interferon and IFNAR play an important role in hepatitis B virus (HBV) infection and anti-HBV therapy. However, its mechanism of action is still poorly understood. To gain more insights into the role of type I interferon and type I interferon receptor (IFNAR) in HBV infection, we established an HBV persistent replication IFNAR knockout (IFNAR(-/-)) mouse model and preliminarily applied this model.

[Assessment of mitochondrial toxicity induced by zidovudine and adefovir dipivoxil in rats].

Objective: To explore the mitochondrial toxicities induced by zidovudine (AZT) and adefovir dipivoxil (ADV) antiviral drugs using a rat model system.

Methods: Twelve healthy Sprague-Dawley rats were randomly divided into three equal groups and treated by oral gavage with zidovudine (125 mg/kg/day), adefovir (40 mg/kg/day), or saline (equal volume) for 28 days. The rats' body weights were measured once a week, and blood was collected every two weeks for blood and biochemical tests.

Expression of neuroglobin in ocular hypertension induced acute hypoxic-ischemic retinal injury in rats.

Aim: To investigate the expression of neuroglobin (Ngb) in the retina of rats with ocular hypertension induced acute retinal hypoxic-ischemic injury.

Methods: Seventy Wistar rats were divided into 7 groups randomly. The experimental model was induced by elevation of intraocular pressure via anterior chamber canula insertion in the left eyes and the fellow eyes were preserved as normal controls.

[Establishment and application of a loop-mediated isothermal amplification method for rapid diagnosis of Vibrio cholerae].

Objective: To establish a loop-mediated isothermal amplification (LAMP) method for rapid diagnosis of Vibrio cholerae.

Methods: Based on the ompW nucleic sequence of Vibrio cholerae, a pair of primers was designed for LAMP. The reaction conditions were optimized, and the specificity, sensitivity, and practicability of LAMP were tested using 47 bacterial strains and simulated contaminated sites.

Quantitative determination of a novel enantiomeric tropane analog, (-)-satropane, in biological fluids using liquid chromatography/tandem mass spectrometry.

A sensitive, accurate and precise liquid chromatography-tandem mass spectrometry method was developed for the determination of (-)-satropane (3alpha-paramethyl-benzenesulfonyloxy-6beta-acetoxy-tropane) in rabbit aqueous humor. Since (-)-satropane may be absorbed from the aqueous humour with resultant systemic side effects, the LC-MS/MS method was also evaluated for its applicability in analyzing plasma samples containing this compound. (-)-Satropane and phentolamine (the internal standard, represented as IS) were detected by multiple reaction monitoring using the transitions m/z 354-182 and 282-212, respectively.

Preparation and evaluation of a novel delayed-onset sustained-release system of propranolol hydrochloride.

The objective of this work was to prepare and evaluate a new delayed-onset sustained-release system, comprising a sustained-release core tablet with hydroxypropyl methylcellulose as polymer matrix and an ethylcellulose/Eudragit L coating capable of delaying the drug release. The sustained core containing propranolol hydrochloride as the model drug was prepared by granulate tableting and the polymer coating was applied in a computer-controlled coating pan. The dissolution tests demonstrated that the in-vitro drug release was pH-dependent with sufficient gastric resistance, and the lag time (t(10%)) could be controlled by adjusting the coating level.

Synergistically enhanced transdermal permeation and topical analgesia of tetracaine gel containing menthol and ethanol in experimental and clinical studies.

The aim of this study is to observe the synergistically enhanced percutaneous penetration and skin analgesia of tetracaine gel containing menthol and ethanol through experimental and clinical studies. Four anesthetic gels containing 4% tetracaine in carbomer vehicle named T-gel (containing no menthol or ethanol), 5%M/T-gel (containing 5% menthol), 70%E/T-gel (containing 70% ethanol, an optimal concentration for antiseptic), and 5%M+70%E/T-gel (containing both 5% menthol and 70% ethanol), respectively, were fabricated. The in vitro mouse skin permeation was investigated using a Franz diffusion cell.

[Design of mechanical structure dynamic characteristics and development of verification system].

Objective: To present an effective method for structural dynamic characteristics design, required design and test means for space products' development.

Method: Computational modal analysis method was used to evaluate natural frequencies and modes of the products in the beginning of design, and test modal analysis method was used to identify the dynamic parameters of products in the test period.

Result: The proposed computational modal analysis system is capable of making static and dynamic analysis, as well as natural frequencies and modes analysis of the structure, and this system could be applied in mechanical structure dynamic characteristics design.

[Development of a shock response spectrum test facility].

Objective: To develop a shock response spectrum test facility for testing space products in the pyrotechnic environments.

Method: The natural frequencies of the resonant plate, a key part of the facility, were calculated with finite element method. The dynamic response of the whole system was simulated when the machine was excited by a real shock generated by a dropping hammer.

Analysis of floral morphology of a cotton homeotic variant (CHV1).

Physiological and genetic variations exist frequently in the cotton plants regenerated from somatic embryos. A stable homeotic variant (CHV1) was identified from these regenerated plants. Morphological analysis suggested that all the floral organs of the CHV1 plants convert into bract leaf-like organs, this was confirmed by close inspection of floral organs surface using scanning electron microscope.

[Development of a measurement and control system of human centrifuge].

Objective: To develop measurement and control system for human centrifuge to simulate arbitrary hypergravity curves.

Method: The human centrifuge is controlled by the upper and lower level computers. The measurement and control computer is as upper, and the real-time controller as lower.