Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation.

Background: Cardiomyocyte growth and differentiation rely on precise gene expression regulation, with epigenetic modifications emerging as key players in this intricate process. Among these modifications, N6-methyladenosine (m6A) stands out as one of the most prevalent modifications on mRNA, exerting influence over mRNA metabolism and gene expression. However, the specific function of m6A in cardiomyocyte differentiation remains poorly understood.

Systematic comparison of tools used for mA mapping from nanopore direct RNA sequencing.

N6-methyladenosine (m6A) has been increasingly recognized as a new and important regulator of gene expression. To date, transcriptome-wide m6A detection primarily relies on well-established methods using next-generation sequencing (NGS) platform. However, direct RNA sequencing (DRS) using the Oxford Nanopore Technologies (ONT) platform has recently emerged as a promising alternative method to study m6A.

High-precision mapping reveals rare N-deoxyadenosine methylation in the mammalian genome.

N-deoxyadenosine methylation (6mA) is the most widespread type of DNA modification in prokaryotes and is also abundantly distributed in some unicellular eukaryotes. However, 6mA levels are remarkably low in mammals. The lack of a precise and comprehensive mapping method has hindered more advanced investigations of 6mA.

The complete genome of cv. dongzao, an economic crop in Yellow River Delta of China.

The complete chloroplast genome of cv. dongzao, known as an important economic cultivar in Yellow River Delta of China was reported. It exhibits a quadripartite structure with 161,493 bp including a large single copy region (89,178 bp), a small single copy region (19,357 bp) and a pair of inverted repeats regions (26,479 bp).

[Effects of Citalopram on frontal cortical neurons' bax mRNA bcl-2 mRNA expression and cell apoptosis of rat after stress].

Objective: To study the effects of Citalopram on the mRNA expression of bax and bel-2 in frontal cortical neurons and on cell apoptosis of rats after stress.

Methods: Twenty-four healthy male SD rats were randomly divided into three groups (n = 8). The control group did no receive any treatment, the stress group was subject to stress and given normal saline and experimental group was given Citalopram irrigation stomach after stress.

Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis.

Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282).

[Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos].

Objective: To investigate the expression of neuron-specific enolase (NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus.

Methods: Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age.

[Expressions of microtubule-associated protein 2 and nestin in the development of human embryo and fetal tongue muscles].

Objective: To explore the role of microtubule-associated protein 2 (MAP-2) and nestin in the development of tongue muscles of human embryos and fetuses.

Methods: PV immunohistochemistry was used to detect the expressions of MAP-2 and nestin proteins in the tongue tissues of human embryos and fetuses at the second, third and fourth months of gestation.

Results: MAP-2 and nestin positivity was detected in the tongue muscles of human embryos at 2 to 4 months of gestation.

[Effects of citalopram on the expression of PCNA and C-fos and cell apoptosis in rat frontal cortical neurons after stress].

Objective: To study the effects of citalopram on the expression of proliferating cell nuclear antigen (PCNA) and proto-oncogene protein (C-fos) and cell apoptosis in frontal cortical neurons of rat after stress.

Methods: Twenty four healthy male SD rats were randomly divided into three groups (n = 8): control group, stress group (treated with saline, ig) , experimental group (treated with Citalopram 4 mg/kg x d for 28 days, ig). Rats were forced to swim to establish chronic stress model.

[Expression of PCNA, C-fos and Bax proteins in human embryonic tongue tissues].

Objective: To investigate of proliferating cell nuclear antigen (PCNA), C-fos and Bax proteins in human embryonic tongue tissue of different developmental stages.

Methods: Immunohistochemistry was used to detect the expressions of PCNA, C-fos and Bax proteins in embryonic tongue tissues of fetuses with 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the number of positive expression cells in tongue tissues of fetuses with different gestational age.

How immunogenetically different are domestic pigs from wild boars: a perspective from single-nucleotide polymorphisms of 19 immunity-related candidate genes.

The coexistence of wild boars and domestic pigs across Eurasia makes it feasible to conduct comparative genetic or genomic analyses for addressing how genetically different a domestic species is from its wild ancestor. To test whether there are differences in patterns of genetic variability between wild and domestic pigs at immunity-related genes and to detect outlier loci putatively under selection that may underlie differences in immune responses, here we analyzed 54 single-nucleotide polymorphisms (SNPs) of 19 immunity-related candidate genes on 11 autosomes in three pairs of wild boar and domestic pig populations from China, Iberian Peninsula, and Hungary. Our results showed no statistically significant differences in allele frequency and heterozygosity across SNPs between three pairs of wild and domestic populations.

Dichlorido{2-[(2,6-dimethyl-phen-yl)imino-meth-yl]pyridine-κN,N'}zinc.

In the asymmetric unit of the title compound, [ZnCl(2)(C(14)H(14)N(2))], the central Zn(II) ion is four-coordinated in a distorted tetra-hedral environment by two N atoms of the ligand 2-[(2,6-dimethyl-phen-yl)imino-meth-yl]pyridine and two chloride anions. In the crystal, adjacent mol-ecules are connected through C-H⋯Cl hydrogen bonds between a C-H group of the ligand and a Cl(-) anion, leading to a chain-like structure along the b direction.

[Expression of nNOS, Pax3 and Cx43 proteins in early developing posterior horn of embryonic and fetal human spinal cord].

Objective: To investigate the distribution pattern of the expressions neuronal nitric oxide synthase (nNOS), Pax3 and connexin 43 (Cx43) proteins in the early developing posterior horn of embryonic and fetal human spinal cord.

Methods: Immunohistochemistry was used to detect the expressions of nNOS, Pax3 and Cx43 proteins in the posterior horn of the spinal cord during the second, third and fourth month of human embryonic and fetal development.

Results: In the second to fourth month of gestation, the expressions of nNOS and Pax3 proteins increased gradually from weak expression to strong expression in the posterior horn of the spinal cord.

[Expression of Cx43 and Pax3 in the small intestinal muscular layers of early human embryos].

Objective: To explore the patterns of Cx43 and Pax3 protein expressions in the small intestinal muscular layers of human embryo during early development.

Methods: Immunohistochemistry with SABC method was employed to examine the expression of Cx43 and Pax3 proteins in the muscular layers of the small intestine in early human embryos in the second to fourth months of gestation.

Results: In the second month of gestation, the muscle layer of the small intestine was negative for Cx43 and Pax3 protein expressions.