[The change in expression of Toll-like receptor 4 on peripheral blood mononuclear cell after continuous renal replacement therapy in patients with sepsis].

Objective: To explore the change in Toll-like receptor 4 (TLR4) of peripheral blood mononuclear cell (PBMC) and the expression of cytokines in patients with sepsis, and to investigate the role of continuous renal replacement therapy (CRRT) in its modulation.

Methods: A clinical study was performed in 13 patients with sepsis which were divided into improved group (n=7) and healing group (n=6), according to their outcome. Another 6 healthy individuals served as normal control.

[Role of trefoil peptides in modulation of gastric adaptation to stress].

Objective: To investigate the role of trefoil peptides in modulation of gastric adaptation to water restraint stress (WRS) in rats.

Methods: Wistar rats were exposed to single or repeated WRS for 4 hours every other day for up to 6 days, gastric mucosal blood flow (GMBF) was measured by LDF-3 flowmeter, the extent of gastric mucosal lesions was evaluated grossly and histologically, and expression of PS2 intestinal trefoil peptide (ITF), cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) and transferase growth factor-alpha (TGF-alpha) were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.

Results: One application of WRS produced extensive gastric mucosal erosion.

Serial analysis of gene expression in mice with lipopolysaccharide-induced acute lung injury.

Objective: To monitor the systemic gene expression profile in a murine model of lipopolysaccharide-induced acute lung injury.

Methods: Acute lung injury was induced by intratracheal injection of lipopolysaccharide in 3 mice. Another 3 normal mice receiving same volume of normal saline were taken as the controls.

Cyclooxygenase 2, pS2, inducible nitric oxide synthase and transforming growth factor alpha in gastric adaptation to stress.

Aim: To determine the role of mucosal gene expression of cyclooxygenase 2 (COX-2), pS2 (belongs to trefoil peptides), inducible nitric oxide synthase (iNOS) and transforming growth factor alpha (TGFalpha) in gastric adaptation to water immersion and restraint stress (WRS) in rats.

Methods: Wistar rats were exposed to single or repeated WRS for 4 h every other day for up to 6 d. Gastric mucosal blood flow (GMBF) was measured by laser Doppler flowmeter-3.

Role of TFF in healing of stress-induced gastric lesions.

Aim: To determine the changes of pS2 and ITF of TFF expression in gastric mucosa and the effect on ulcer healing of pS2, ITF to Water-immersion and restraint stress (WRS) in rats.

Methods: Wistar rats were exposed to single or repeated WRS for 4 h every other day for up to 6 days.Gastric mucosal blood flow (GMBF) was measured by LDF-3 flowmeter and the extent of gastric mucosal lesions were evaluated grossly and histologically.