Enhanced proton conductivity by guest molecule exchange in an acylamide-functionalized metal-organic framework.

Metal-organic frameworks (MOFs) as types of proton conductive materials have attracted much attention. Here, an acylamide-functionalized 3D MOF, [Ni(TPBTC)(stp)(HO)]·2DMA·32HO, has been successfully constructed combining Ni(NO), TPBTC (TPBTC = benzene-1,3,5-tricarboxylic acid tris-pyridin-4-ylamide) and 2-Hstp (2-Hstp = 2-sulfoterephthalic acid monosodium salt) under solvothermal conditions. Single-crystal X-ray diffraction revealed that there are uncoordinated guest DMA molecules in the pores of the compound.

Three layered cucurbit[6]uril-based metal-organic rotaxane networks functionalized by sulfonic groups for proton conduction.

Three new cucurbit[6]uril (CB[6])-based metal-organic rotaxane networks (MORNs) (named CUST-711, CUST-712, and CUST-713) functionalized by a sulfonic group (-SOH) have been designed and synthesized a hydrothermal method. All three compounds exhibited similar two-dimensional (2D) wave layer structures. Their stability under different temperature and relative humidity conditions has been investigated and all the compounds showed excellent stability.

Syntheses of cucurbit[6]uril-based metal-organic rotaxane networks by the anion regulation strategy and their proton conduction properties.

Two new cucurbit[6]uril (CB[6])-based metal-organic rotaxane networks (MORNs) were successfully obtained by tuning the coordination sphere of metal copper clusters. Compounds 1 and 2 exhibited relatively high proton conductivity at 85 °C and 97% relative humidity (RH), providing great promise for fuel cell electrolyte materials.

Syntheses and magnetic properties of high-dimensional cucurbit[6]uril-based metal-organic rotaxane frameworks.

Three new high-dimensional cucurbit[6]-based metal-organic rotaxane frameworks [Co(PR43)(BDC)Cl]·4HO (1), [Co(PR43)(BTC)]·6HO (2) and [Co(PR43)(BPT)]·20HO (3) were obtained via the hydrothermal synthesis method. Compound 1 comprised a two-dimensional layered structure, while compounds 2 and 3 exhibited three-dimensional pillared structures. All the compounds showed good thermal stabilities.

Effects of Brain Temperature on the Outcome of Patients with Traumatic Brain Injury: A Prospective Observational Study.

A prospective observational study collected temperature data from 51 patients in 11 neurosurgical centers and follow-up outcome information at 6 months in 49 patients. Brain temperature (T) was measured directly by an intraventricular temperature sensor. Axillary temperature (T) and rectal temperature (T) were measured by electric thermometers.

Effect and safety of sorafenib in patients with intermediate hepatocellular carcinoma who received transarterial chemoembolization: A retrospective comparative study.

Aim: To evaluate the safety and efficacy of sorafenib plus transarterial chemoembolization (TACE) treatment for intermediate hepatocellular carcinoma (HCC).

Methods: Sixty-seven patients with intermediate-stage [Barcelona Clinic liver cancer stage B (BCLC-B)] HCC who were treated with sorafenib plus TACE or TACE alone between 2009 and 2011 were included in the study. Follow-up was until 2014 or patient death.

Guest exchange in a porous cucurbit[6]uril-based metal-organic rotaxane framework probed by NMR and X-ray crystallography.

The first CB[6]-based 3D porous metal-organic rotaxane framework is constructed by the reaction of CuCl2, terephthalic (H2BDC) and CB[6]-based [2]pseudorotaxanes ([PR44]2+·2[PF6]-) under solvothermal conditions. The structure of MORF-1 is a pillared-layer structure with 5-connected sqp topology, in which the effective free volume is 45.4% of the crystal volume.

Hint1 suppresses migration and invasion of hepatocellular carcinoma cells in vitro by modulating girdin activity.

Histidine triad nucleotide-binding protein 1 (Hint1) is a haploinsufficient tumor suppressor gene. Its role in cancer cell migration has not been previously speculated. In the current study, we examined the expression of Hint1 in metastatic and non-metastatic lymph nodes of hepatocellular carcinoma (HCC) patients and further elucidated the effect of Hint1 expression on girdin expression and phosphorylation of AKT and ERK1/2 and on the migration of HCC cells in vitro.

A novel TP53 variant (rs78378222 A > C) in the polyadenylation signal is associated with increased cancer susceptibility: evidence from a meta-analysis.

Polymorphisms in TP53 are involved in the progression of different types of cancer. A rare novel TP53 variant (rs78378222 A > C allele) was found via whole-genome sequencing in 2011. This variant was shown to significantly increase the risk of glioma, colorectal adenoma and prostate cancer.

Polyoxovanadate-based organic-inorganic hybrids: from {V5O9Cl} clusters to nanosized octahedral cages.

Three polyoxovanadate-based metal-organic polyhedra (denoted as VMOP-1, -2, and -3), adopting isostructural discrete octahedral cage geometries, were successfully synthesized under solvothermal conditions. These structures are all built up from the same pentavanadate {V5O9Cl} cluster connected by linear bidentate ligands (H2L1 = H2BDC, H2L2 = H2BDC-NH2, H2L3 = H2BDC-Br), respectively.

Interleukin-1B 31 C>T polymorphism combined with Helicobacter pylori-modified gastric cancer susceptibility: evidence from 37 studies.

Gastric cancer is one of the most common malignancies worldwide. Interleukin-1-beta (IL-1β) is a pro-inflammatory cytokine and potent inhibitor of gastric acid secretion. Some studies provided evidence of the association between IL-1B 31 polymorphism and gastric cancer risk while other studies did not.

Spontaneous chiral resolution of a rare 3D self-penetration coordination polymer for sensitive aqueous-phase detection of picric acid.

Two unprecedented homochiral enantiomers based on two different kinds of rigid ligands, namely [Cd(NDC)L]2·H2O (1R and 1L), have been synthesized under hydrothermal conditions through spontaneous resolution. Their structures were determined by single-crystal X-ray diffraction analysis and further characterized by elemental analysis, IR, and thermogravimetric (TG) analysis. The resulting framework 1, constructed by four kinds of homo-handed helical chains represents the first 3D self-penetrating framework formed by decoration of single (10,3)-a net with helical chains.

An organic-inorganic hybrid photocatalyst based on sandwich-type tetra-Co-substituted phosphotungstates with high visible light photocatalytic activity.

A novel 3D organic-inorganic hybrid framework constructed from tetra-Co(II)-substituted sandwich-type phosphotungstates with a rare 8-connected bcu topology is reported, which exhibited highly efficient photocatalysis activity under visible light and could be used for 5 cycles without any obvious decrease in activity.

Periodic tiling of triangular and square nanotubes in a cationic metal-organic framework for selective anion exchange.

A unique cationic metal-organic framework was synthesized by connecting the neutral rod-shaped secondary building unit with a cationic dicarboxylate ligand. This framework showed a rare snub square tessellation pattern by the periodic tiling of triangular and square nanotubes. The charge- and size-dependent ion-exchange of anion dyes was investigated.

The copper(I) metal azolate framework showing unusual coordination mode for the 1,2,4-triazole derivative and photocatalytic activity.

A unique three-dimensional metal azolate framework containing a tetranuclear copper cluster constructed by six 1,2,4-triazole units was synthesized in which the 1,2,4-triazole units show unusual bridging "crevice" coordination mode with their 1- and 2-positioned sp(2) N-atoms as symmetrically bridging centers. The photocatalytic activities of as-prepared compound were tested by degradation of rhodamine-B (RB) under different light irradiation.

Direct substitution of primary allylic amines with sulfinate salts.

The NH(2) group in primary allylic amines was substituted directly by sulfinate salts with excellent regio- and stereoselectivities. In the presence of 0.1 mol % [Pd(allyl)Cl](2), 0.

Catalytic asymmetric cleavage of sp3 C-N bonds for access to highly enantioenriched N-benzylic sulfonamides.

In the presence of 10 mol% of a chiral phosphoric acid, a variety of racemic N-benzylic sulfonamides having N-(3-indolyl)methyl groups smoothly undergo kinetic resolution with benzyl thiol at 0 °C or at room temperature and the remaining sulfonamides are recovered in moderate to excellent yields and with excellent ee.

Inhibition of SIRT1 increases EZH2 protein level and enhances the repression of EZH2 on target gene expression.

Objective: To study the regulatory rolesof SIRT1 on EZH2 expression and the further effects on EZH 2’ s repression of target gene expression.

Methods: The stable SIRT1 RNAi and Control RNAi HeLa cells were established by infection with retroviruses expressing shSIRT1 and shLuc respectively followed by puromycin selection. EZH2 protein level was detected by Western blot in either whole cell lysate or the fractional cell extract.

Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin.

A wide variety of nuclear regulators and enzymes are subjected to acetylation of the lysine residue, which regulates different aspects of protein functions. The MYST family histone acetyltransferase, human ortholog of MOF (hMOF), plays critical roles in transcription activation by acetylating nucleosomal H4K16. In this study, we found that hMOF acetylates itself in vitro and in vivo, and the acetylation is restricted to the conserved MYST domain (C2HC zinc finger and HAT), of which the K274 residue is the major autoacetylation site.

Epigenetic repression of SATB1 by polycomb group protein EZH2 in epithelial cells.

Objective: To study the regulatory mechanism of SATB1 repression in cells other than T cells or erythroid cells, which have high expression level of SATB1.

Methods: HeLa epithelial cells were treated with either histone deacetylase inhibitor (HDACi) trichostatin A (TSA) or DNA methylation inhibitor 5-Aza-C before detecting SATB1 expression. Luciferase reporter system was applied to measure effects of EZH2 on SATB1 promoter activity.

Cutaneous T-cell lymphoma: roles for chemokines and chemokine receptors.

Chemokine receptors are G-protein-coupled, seven-transmembrane-spanning surface receptors that play key roles in cell trafficking, cell motility, and survival. These receptors are activated by small molecular weight chemotactic cytokines called chemokines. Chemokine receptors play roles in the migration and localization of normal T cells (and other leukocytes) during physiological responses in inflamed or infected skin.

Improvement of SSO-mediated gene repair efficiency by nonspecific oligonucleotides.

Targeted gene repair mediated by single-stranded DNA oligonucleotides (SSOs) is a promising method to correct the mutant gene precisely in prokaryotic and eukaryotic systems. We used a HeLa cell line, which was stably integrated with mutant enhanced green fluorescence protein gene (mEGFP) in the genome, to test the efficiency of SSO-mediated gene repair. We found that the mEGFP gene was successfully repaired by specific SSOs, but the efficiency was only approximately 0.

Identification of long range regulatory elements of mouse alpha-globin gene cluster by quantitative associated chromatin trap (QACT).

Chromatin from different regions of the genome frequently forms steady associations that play important roles in regulating gene expression. The widely used chromatin conformation capture (3C) assay allows determination of the in vivo structural organization of an active endogenous locus. However, unpredicted chromatin associations within a given genomic locus can not be identified by 3C.

MafK/NF-E2 p18 is required for beta-globin genes activation by mediating the proximity of LCR and active beta-globin genes in MEL cell line.

Evidences indicate that locus control region (LCR) of beta-globin spatially closes to the downstream active gene promoter to mediate the transcriptional activation by looping. DNA binding proteins may play an important role in the looping formation. NF-E2 is one of the key transcription factors in beta-globin gene transcriptional activation.