Singlet oxygen induces cell wall thickening and stomatal density reducing by transcriptome reprogramming.

Singlet oxygen (O) has a very short half-life of 10 s; however, it is a strong oxidant that causes growth arrest and necrotic lesions on plants. Its signaling pathway remains largely unknown. The Arabidopsis flu (fluorescent) mutant accumulates a high level of O and shows drastic changes in nuclear gene expression.

Two new photoactive metal-organic compounds for degradation of methylene blue and treatment in ophthalmic local anesthesia.

Two new metal-organic compounds, namely [Cu(CrO)(4,4'-bipy)(HO)]·n(HO) (1) together with [Mn(CrO)(bpp)] (2) (4,4'-bipy is 4,4'-bipyridine and bpp is 1,3-bis(4-pyridyl)propane), were hydrothermally generated, which were characterized structurally through a series of characterization techniques. Moreover, compounds 1 and 2 have 2.95 eV and 3.

[Spatial Characteristics and Potential Ecological Risk Factors of Heavy Metals in Cultivated Land in the Transition Zone of a Mountain Plain].

The purpose of this study was to explore the spatial distribution characteristics and potential ecological risk factors of heavy metal pollution in cultivated soil in the transitional zone of a typical mountain plain. In this study, a county in Sichuan Province, which has the typical transitional topography of the Chengdu Plain-Longmen Mountain transition zone, was taken as the study area. Geostatistics and potential ecological risk index methods were used to analyze the spatial distribution of Cd, Cr, Cu, Pb, and Zn from cultivated land in the study area, and a potential ecological risk assessment was carried out.

Prediction models for development of hepatocellular carcinoma in chronic hepatitis B patients.

Chronic hepatitis B (CHB)-related hepatocellular carcinoma (HCC) is a major health problem in Asian-Pacific regions. Antiviral therapy reduces, but does not completely prevent, HCC development. Thus, there is a need for accurate risk prediction to assist prognostication and decisions on the need for antiviral therapy and HCC surveillance.

[Effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats].

Objective: To observe the effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats.

Methods: The rat models of myalgia comorbid depression were prepared by intraperitoneal injection of acute reserpine. Twenty-four SD male rats were randomly divided into control group, model group, small needle knife group and amitriptyline group, 6 rats in each group.

Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system.

Aim: To investigate the biological function of F protein by yeast two-hybrid system.

Methods: We constructed F protein bait plasmid by cloning the gene of F protein into pGBKT7, then recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (alpha type) containing liver cDNA library plasmid in 2XYPDA medium.

[Screening and cloning target genes transactivated by hepatitis C virus F protein using suppression subtractive hybridization technique].

Objectives: To identify and clone human genes transactivated by HCV F protein by constructing a cDNA subtractive library using the suppression subtractive hybridization technique.

Methods: Suppression subtractive hybridization (SSH) and bioinformatics techniques were used for screening and cloning of the target genes transactivated by HCV F protein. The mRNA was isolated from HepG2 cells transfected with pcDNA3.

Screening of genes of proteins interacting with p7 protein of hepatitis C virus from human liver cDNA library by yeast two-hybrid system.

Aim: To investigate the biological function of p7 protein and to look for proteins interacting with p7 protein in hepatocytes.

Methods: We constructed p7 protein bait plasmid by cloning the gene of p7 protein into pGBKT7, then transformed it into yeast AH109 (a type). The transformed yeast was mated with yeast Y187 (alpha type) containing liver cDNA library plasmid, pACT2 in 2XYPDA medium.

[Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2].

Objective: To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.

Methods: Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418.

[Application of continuous epidural analgesia with ropivacaine and fentanyl during delivery].

Objective: To evaluate the safety and efficiency of ropivacaine and fentanyl for continuous epidural analgesia during delivery.

Methods: Altogether 98 full-term primigravidas with vertex presentation were selected for this study. Epidural catheter was placed during delivery with the cervical dilation of 3 cm, and the mixture of 0.