The multicomponent residue depletion of in pig tissues, urine, and plasma.

Introduction: () as a traditional medicinal plant used in livestock production. The use of in veterinary clinics may pose safety risks to human health.

Objectives: The aim of this study was to investigate tissue residue depletion in pigs fed powder.

Toxicity assessment of gelsenicine and the search for effective antidotes.

Background: Gelsenicine, one of the most toxic alkaloids of Benth (), causes severe respiratory depression. However, its toxicity mechanisms are yet to be elucidated and no effective antidotes are available.

Objective: This study aimed to analyse the toxicity characteristics of gelsenicine.

Comparative toxicokinetic profiles of multiple-components of Gelsemium elegans in pigs and rats after a single oral administration.

Gelsemium elegans Benth (G. elegans) is highly toxic to humans and rats, but has insecticides and growth promoting effects on pigs and goats. G.

An analytical strategy to explore the multicomponent pharmacokinetics of herbal medicine independently of standards: Application in Gelsemium elegans extracts.

The multicomponent pharmacokinetic study of herbal medicine is a great challenge due to the low plasma concentrations, large range of concentration scales, lack of authentic standards and uncertain interactions of the components. The aim of this work was to explore the in vivo pharmacokinetics of herbal medicine independently of authentic standards using an integrated analytical strategy. First, ion pairs of multiple components were tuned and selected, and then major parameters were optimized for derivative multiple reaction monitoring (DeMRM) by LC-MS/MS, which was combined with characterization of the chemical profiles of the herbal medicine by LC-QqTOF/MS.

Development and in-house validation of a sensitive LC-MS/MS method for simultaneous quantification of gelsemine, koumine and humantenmine in porcine plasma.

Three monomers of G. elegans indole alkaloids (gelsemine, koumine and humantenmine) were simultaneously detected in porcine plasma for the first time with the development and validation of a sensitive and reliable LC-ESI-MS/MS method. Using a gradient mobile phase at a constant flow rate of 0.