Publications by authors named "Xue-Min Cai"
Article Synopsis
- The study investigates the effects of mouse umbilical cord mesenchymal stem cells (mUCMSCs) on the reproductive health of ageing C57 mice, focusing on their potential to repair ovarian function.
- Aging mice with diminished ovarian function received mUCMSC injections, resulting in increased ovarian size, the presence of follicles, and enhanced hormone levels compared to a control group.
- The findings indicate that mUCMSCs can reduce cell apoptosis and oxidative stress in granulosa cells by altering specific gene expressions and activating key cellular pathways, suggesting a potential for regenerative therapies in reproductive health.
Based on the characteristics of modern weapon injury, a repetitive model of traumatic systemic inflammatory response syndrome (SIRS) and an evaluation system were established. The models were treated with GFP-labeled tree shrew umbilical cord mesenchymal stem cells (UCMSCs). Forty out of 50 tree shrews were used to make a unilateral femoral comminuted fracture.
View Article and Find Full Text PDFSelf-renewal of embryonic stem cells (ESCs) is orchestrated by a vast number of genes at the transcriptional and translational levels. However, the molecular mechanisms of post-translational regulatory factors in ESC self-renewal remain unclear. Histidine phosphorylation, also known as hidden phosphorylation, cannot be detected by conventional experimental methods.
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- Ischemia-reperfusion injury significantly contributes to acute kidney injury and affects recovery after kidney transplants; this study explores the potential protective role of stem cell transplantation in such injuries.
- The experiment involved 40 rabbits divided into four groups, with 30 establishing the injury model, and 10 serving as untreated controls; comparisons were made based on treatments with either induced or non-induced peripheral blood mononuclear cells (PBMCs).
- Results showed that rabbits treated with induced PBMCs exhibited improved renal function and less severe pathological changes, indicating the therapeutic potential of these induced stem cells in mitigating kidney injury.
Article Synopsis
- A mouse model for allergic rhinitis (AR) was developed using BALB/c mice to aid in future research on the condition.
- * The study involved preparing human umbilical cord mesenchymal stem cells (hUCMSCs), which were shown to be effective for tracking in animal studies and possibly for AR treatment.
- * The transplantation of hUCMSCs, particularly through tail vein injection, demonstrated positive effects on the AR model, with a significant presence of these cells in the nasal area after two weeks.
Article Synopsis
- The study aimed to investigate how allogeneic umbilical cord mesenchymal stem cell (mUCMSC) transplantation affects the thymus in aging mice, potentially offering a new treatment for age-related thymic atrophy.
- Results indicated that mUCMSC treatment improved thymus structure and function by analyzing changes in thymic tissue and immune cell markers, as well as promoting hair regeneration in the mice.
- The findings suggest that mUCMSCs can reduce thymus aging by affecting specific senescence-related genes, though further research is needed to fully understand the underlying mechanisms.
Article Synopsis
- Inflammatory bowel disease (IBD) causes chronic inflammation in the gastrointestinal tract, and researchers are exploring new treatments with minimal side effects.
- Mouse models of IBD were created using a solution called DSS, allowing scientists to test the effects of umbilical cord mesenchymal stem cells (UCMSCs) from both mice and humans.
- The study found that UCMSCs improve survival rates and promote healing by enhancing key proteins in the intestines, offering a promising avenue for future IBD therapies.
Article Synopsis
- - The study explored the link between bone marrow mesenchymal stem cells (BMSCs) and aging by establishing an aging macaque model, analyzing how BMSCs from young and aged macaques differ in their characteristics.
- - Researchers found that aging BMSCs showed decreased levels of key genes associated with aging, such as TERT, SIRT1, and SIRT6, using techniques like RT-PCR and Western blot.
- - The team compared the effects of young and aged BMSCs on 293T cells, investigated secreted cytokines, and analyzed transcriptomes of peripheral blood mononuclear cells from macaques to understand gene transcription and regulatory mechanisms related to aging.
Article Synopsis
- * This study explored the use of islet-like cells from umbilical cord mesenchymal stem cells (UC-MSCs) in tree shrews with type 2 diabetes, showing effectiveness in reducing disease severity.
- * UC-MSCs were found to differentiate into functional insulin-secreting cells through stimulation from high glucose and activation of Notch signaling, making this method a potentially simple and cost-effective diabetes treatment.
Article Synopsis
- Embryonic-like stem cells (ELSCs) show potential in muscle regeneration, expressing markers for embryonic stem cells and differentiating into various cell types more effectively than mesenchymal stromal cells (MSCs).
- In a study using dystrophin/utrophin double knock-out mice (a model for Duchenne muscular dystrophy), ELSCs were injected via tail vein and led to improved motor function and reduced creatine kinase levels two months post-transplant.
- Results indicated that ELSCs enhanced dystrophin expression and muscle tissue quality compared to MSCs, supporting their potential for therapeutic use in muscle disorders.
Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin.
View Article and Find Full Text PDFIntroduction: Renal interstitial fibrosis (RIF) is a significant cause of end-stage renal failure. The goal of this study was to characterize the distribution of transplanted induced autologous stem cells in a rabbit model of renal interstitial fibrosis and evaluate its therapeutic efficacy for treatment of renal interstitial fibrosis.
Methods: A rabbit model of renal interstitial fibrosis was established.
Article Synopsis
- The study explored the impact of induced autologous stem cells on blood sugar levels in rabbits with type 1 diabetes by transplanting dedifferentiated fibroblasts into the pancreatic artery.
- After four weeks, the treatment group showed significantly lower blood glucose levels and increased plasma insulin and C-peptide levels, indicating improved pancreatic function.
- The morphological analysis revealed that induced stem cells led to changes in islet cell structure, with better preservation and function compared to the non-induced treatment group and control group, suggesting potential for diabetes therapy.
The objective of this study was to investigate the effects of transplanted bone marrow mesenchymal stem cells (BMSCs) administered via internal jugular vein injection, carotid artery injection, or intraventricular transplantation for the treatment of cerebral infarction, which was modeled in rats. The neurological scores of the treated rats and the distribution of the transplanted cells (GFP-labeled) in the infarction area were evaluated. The cerebral infarction model was produced by inserting a modified Zea-longa suture, which generated middle cerebral artery occlusion (MCAO).
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- The study investigated how different egg extracts affect the survival and differentiation of spleen cells from male GFP-transgenic mice after being permeabilized for 30 minutes.
- Among the five egg extracts tested, chicken-egg-white and whole-egg extracts significantly enhanced the survival and differentiation of the spleen cells compared to others, with GFP-positive cells detected in female mice post-transplantation.
- The findings suggest that chicken-egg-white and whole-egg extracts could be valuable in future cellular biology research, particularly in maintaining stem cell function.
Article Synopsis
- Multipotent stem cells show promise for regenerative medicine, but their limited availability poses challenges for clinical use.
- Researchers developed an in vitro method to reprogram skin fibroblasts into multipotent cells, activating important stem cell markers like Oct4 and Nanog.
- The reprogrammed cells can differentiate into various cell types and form teratomas, while maintaining genomic imprinting, offering a safer alternative to traditional iPS cell methods.
[Prokaryotic expression of N terminal fragment of mannan-binding lectin associated serine protease-1].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
June 2008
Aim: To express the N-terminal fragment of human mannan-binding lectin (MBL) associated serine proteases-1 (MASP1-N) in E.coli.
Methods: The target sequence in pGEM-MASP1 plasmid that contains human MBL-MASP1 cDNA was amplified by PCR, inserted into prokaryotic expression vector pGEX4T-1 and identified by restriction mapping and sequencing.
[Construction of the expression vector pET32a/His MBL-CLR and its expression in E.coli].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
January 2007
Aim: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E.coli
Methods: The human MBL-CLR gene was amplified by PCR from pGEM-MBL plasmid, and was inserted into prokaryotic expression vector pET32a. After identified by restriction mapping and sequencing, the recombinant plasmid pET32a/His MBL-CLR was transformed into E.