COVID-19-another influential event impacts on laboratory medicine management.

Background: Before public health emergencies became a major challenge worldwide, the scope of laboratory management was only related to developing, maintaining, improving, and sustaining the quality of accurate laboratory results for improved clinical outcomes. Indeed, quality management is an especially important aspect and has achieved great milestones during the development of clinical laboratories.

Current Status: However, since the coronavirus disease 2019 (COVID-19) pandemic continues to be a threat worldwide, previous management mode inside the separate laboratory could not cater to the demand of the COVID-19 public health emergency.

Different binding properties of two general-odorant binding proteins in Athetis lepigone with sex pheromones, host plant volatiles and insecticides.

Athetis lepigone (Alep) is a polyphagous pest native to Europe and Asia that has experienced major outbreaks in the summer maize area of China since 2011 and has shown evidence of resistance to some insecticides. Insect olfaction is crucial for recognition of sex pheromones, host plant volatiles and even insecticides, in which two general-odorant binding proteins (GOBPs) play important roles. To elucidate the functions of GOBPs in A.

Circulating tumor DNA detectable in early- and late-stage colorectal cancer patients.

Characterization, diagnosis, and treatment of colorectal cancers (CRC) is difficult due to limited biopsy information, impracticality of repeated biopsies, and cancer biomarker fallibility. Circulating tumor DNA (ctDNA) has recently been investigated as a non-invasive way to gain representative gene mutations in tumors, in addition to monitoring disease progression and response to treatment. We analyzed ctDNA mutations and concentrations in 47 early- and late-stage CRC patients using a targetted sequencing approach using a panel that covers 50 cancer-related genes.

Rapid and Large-Scale Synthesis of IRMOF-3 by Electrochemistry Method with Enhanced Fluorescence Detection Performance for TNP.

Rapid and large-scale synthesis of metal-organic frameworks (MOFs) materials is of great significance for their practical applications. For the first time, we have electrochemically synthesized IRMOF-3 at room temperature by applying a voltage to a zinc electrode immersed in electrolyte containing 2-aminoterephthalic acid (NH-HBDC). The reaction conditions, including the ratio of solvent (electrolyte), the applied voltage, and different reaction times, were investigated and optimized.

[Study on the axial strain sensor of birefringence photonic crystal fiber loop mirror based on the absolute integral of the monitoring peak].

In the present paper, the theoretical expression of the wavelength change and the axial strain of birefringence fiber loop mirror is developed. The theoretical result shows that the axial strain sensitivity of birefringence photonic crystal fiber loop mirror is much lower than conventional birefringence fiber loop mirror. It is difficult to measure the axial strain by monitoring the wavelength change of birefringence photonic crystal fiber loop mirror, and it is easy to cause the measurement error because the output spectrum is not perfectly smooth.

Molecular structure and vibrational bands and 13C chemical shift assignments of both enmein-type diterpenoids by DFT study.

We report here theoretical and experimental studies on the molecular structure and vibrational and NMR spectra of both natural enmein type diterpenoids molecule (6, 7-seco-ent-kaurenes enmein type), isolated from the leaves of Isodon japonica (Burm.f.) Hara var.

Characterization, cloning, and heterologous expression of a subtilisin-like serine protease gene VlPr1 from Verticillium lecanii.

The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles.

Comparison of long-term immunogenicity (23 years) of 10 μg and 20 μg doses of hepatitis B vaccine in healthy children.

To compare the long-term immunogenicity and seroprotection rates in healthy children following 23 years of vaccination with 10 μg or 20 μg doses of plasma-derived hepatitis B vaccine, we revisited all participants from our previous randomized controlled trial. At year 23, 81 participants were tested for HBV serological markers and HBV-DNA, and a booster dose was given to those with anti-HBs titer < 10 mIU/mL. After eliminating the interference of a Year 11 booster dose and vaccines received outside of the trial, around 50% of participants still maintained anti-HBs titers ≥ 10 mIU/mL in both 10 μg and 20 μg groups (p > 0.

[Effect of plasma-derived hepatitis B vaccine 23(rd) year after a full course of vaccination in healthy young children].

Objective: The aim of the study was to evaluate the anti-HBs persistence and the long term preventive efficacy after vaccination 23 years with plasma-derived hepatitis B vaccine.

Methods: The study consisted of 261 children who were 5 - 9 years aged, from two primary schools in two townships of Xi'an. 126 children were randomly selected as vaccine group, and 135 children in control group.

Antibody levels and immune memory 23 years after primary plasma-derived hepatitis B vaccination: results of a randomized placebo-controlled trial cohort from China where endemicity is high.

The duration of protection of hepatitis B vaccine remains incompletely understood. To assess the long-term protection provided by a primary vaccine series, the current study again recruited all subjects of a previous randomized placebo-controlled trial cohort 23 years after vaccination. Two hundred and sixty-one healthy children aged 5-9 years living in a highly HBV-endemic country were enrolled in the primary trial and received three doses of plasma-derived vaccine or placebo.

A cost-effectiveness analysis of universal childhood hepatitis A vaccination in China.

The socioeconomic improvement has impacted hepatitis A virus (HAV) infection with a shift from high to intermediate endemicity in many parts of China. The first China-developed inactivated hepatitis A vaccine, with significantly low price, was licensed in 2002, prompting us to evaluate whether universal childhood vaccination is advisable now in China. We considered vaccination scheduled at ages 12 and 18 months for all healthy children, and assumed that a single cohort was enrolled in 2005.

[Analysis of clinical manifestations of subheath status in a medical college of Guangdong Province].

Objective: To explore the clinical manifestation of subhealth status in teachers and medical staff.

Methods: An on-the-spot investigation was conducted in 891 personnel of a medical college using a self-designed subhealth questionnaire. The diagnostic criteria for subhealth status were formulated on the basis of literature review and expert counseling.

[Evaluation of the questionnaire for subhealth status survey based on the symptoms in traditional Chinese medicine].

Objective: To evaluate the reliability and validity of the questionnaire we designed for subhealth status survey based on the symptoms described in traditional Chinese medicine.

Method: By on-the-spot investigation, the recovery rate of the questionnaires was 96.3% and the response rate of the items was 97.

[A five-year observation on efficacy of revaccination in children of non-response to hepatitis B vaccine].

Objectives: To evaluate the long-term efficacy of revaccination in non-responder children to primary hepatitis B (HB) vaccination and to compare the efficacy of low-dose intradermal inoculation to that of routine-dose intramuscular inoculation.

Methods: 40 healthy non-responder children to primary HB vaccination identified by screening were given a three-dose revaccination randomly by intramuscular (n = 17, 10 microg per dose) or intradermal route (n = 23, 2 microg per dose) since September, 1999, and their blood specimens were collected regularly for testing for HB virus markers up to five years. Another 80 responder children to primary HB vaccination were also followed-up as controls without revaccination.

Hepatitis B revaccination in healthy non-responder Chinese children: five-year follow-up of immune response and immunologic memory.

To assess persistence of anti-HBs and immunologic memory of non-responders after revaccination, 40 healthy non-responder children were given a three-dose recombinant hepatitis B vaccine revaccination randomly by intramuscular (10 microg per dose) or intradermal (2 microg per dose) route and followed up to five years. All 17 intramuscular and 22 of 23 intradermal children developed a seroprotective antibody response (anti-HBs>or=10 mIU/mL) after revaccination. Children of intramuscular group had significantly higher seroprotection rates and anti-HBs geometric mean titers than the intradermal group.

[Expressions of hTERT, p53, c-erbB-2 and Bcl-2 in colonic adenocarcinoma mouse models with liver metastases: effects of classical traditional Chinese medicine prescriptions for promoting circulation and removing blood stasis].

Objective: To observe the effects of classical traditional Chinese medicine prescriptions that function to promote blood circulation and removing blood stasis on the metastatic behavior of malignant tumors, and explore the possible mechanisms of such effects.

Methods: BALB/c mouse models of colonic adenocarcinoma with liver metastases were established by intrasplenic injection of colonic adenocarcinoma cells (CT26) to receive treatment with the three representative classical circulation-promoting and blood stasis-removing prescriptions of traditional Chinese medicine, namely Guizhifuling pills, Didang Tang and Danshenyin, respectively. The expressions of human telomerase reverse transcriptase (hTERT), p53, c-erbB-2 and Bcl-2 were detected in the mouse models with different treatments and in normal control mice.

[Study on the hemoglobin levels of children under the age of three years and the prevalence of anemia at high altitude in Tibet of China].

Objective: To study the hemoglobin status of children under the age of three years and the prevalence of anemia at high altitude.

Methods: Cross-sectional study and randomly sampling were used. Blood was collected on tip of finger and the level of hemoglobin was measured using B-Hemoglobin photometer.

[The introduction of complementary food of children under the age of three years in Tibet of China].

Objective: To understand the feeding status of Tibetan children aged below three years old.

Methods: Cross-sectional study and randomly sampling were used. The information on complementary food was obtained through the recollection from mother of the child.