Enhancement of FK520 production in Streptomyces hygroscopicus var. ascomyceticus ATCC 14891 by overexpressing the regulatory gene fkbR2.

Ascomycin (FK520) is a 23-membered macrolide antibiotic primarily produced by the Streptomyces hygroscopicus var. ascomyceticus. Structurally similar to tacrolimus and rapamycin, it serves as an effective immunosuppressant widely used in the treatment of rejection reactions after organ transplantation and certain autoimmune diseases.

Effects of ultrasound-assisted low-salt curing on water retention, tenderness and in vitro digestive characteristics of grass carp (Ctenopharyngodon Idellus).

The implementation of innovative techniques to achieve low-salt strategies in cured products is a critical issue faced by the food industry. This study aimed to investigate the impact of ultrasound treatment on the quality of the low-salt air-dried fish. The results showed that compared to traditional liquid curing, ultrasound-assisted curing significantly increased the NaCl transfer rate, improved tenderness, and improved water retention and in vitro digestibility (p < 0.

Cooperative integration of spatially resolved multi-omics data with COSMOS.

Recent advancements in biological technologies have enabled the measurement of spatially resolved multi-omics data, yet computational algorithms for this purpose are scarce. Existing tools target either single omics or lack spatial integration. We generate a graph neural network algorithm named COSMOS to address this gap and demonstrated the superior performance of COSMOS in domain segmentation, visualization, and spatiotemporal map for spatially resolved multi-omics data integration tasks.

Insights and perspectives of chitosan-based hydrogels for the removal of heavy metals and dyes from wastewater.

Water pollution has become an increasingly serious issue, necessitating the design and development of more effective wastewater treatment methods. Chitosan-based hydrogels, owing to their unique structural and chemical properties, have demonstrated high efficiency in removing contaminants. However, the application remains restricted by the scarcity of effective adsorption sites and limited environmental stability.

Endoscopic Clips Marking for Transcatheter Arterial Embolization in Refractory Peptic Ulcer Bleeding Patients: A Cohort Study.

Background And Aims: Transcatheter arterial embolization (TAE) is recommended for refractory peptic ulcer bleeding. There are 46% of patients showed no detectable contrast extravasation during TAE. Endoscopic clip in bleeding lesion is visible and could be used as a marker in TAE.

Enhancing the Built-in Electric Field in Oxygen Vacancies-Enriched I-Doped BiOCl for Visible Light-Driven Photocatalytic Oxidation.

In semiconductor catalysts, rational doping of nonmetallic elements holds significant scientific and technological importance for enhancing photocatalytic performance. Here, using a one-step hydrothermal technique, we synthesized iodine-doped BiOCl composite and evaluated the impact of iodine doping on its photocatalytic capability for organic dye degradation under visible light irradiation. In this study, we demonstrate that the introduction of iodide ions not only provides an ideal built-in electric field (BIEF) for BiOCl but also induces the generation of additional oxygen vacancies (OVs).

Treating acute lung injury through scavenging of cell-free DNA by cationic nanoparticles.

Acute lung injury (ALI) and acute respiratory distress syndrome are life-threatening conditions induced by inflammatory responses, in which cell-free DNA (cfDNA) plays a pivotal role. This study investigated the therapeutic potential of biodegradable cationic nanoparticles (cNPs) in alleviating ALI. Using a mouse model of lipopolysaccharide-induced ALI, we examined the impact of intravenously administered cNPs.

HS-SPME-GC-MS combined with relative odor activity value identify the key aroma components of flowery and fruity aroma in different types of GABA tea.

Anaerobic processing is a crucial factor influencing the formation of flavor quality in Gamma-aminobutyric acid (GABA) tea. In this study, headspace solid-phase microextraction combined with gas chromatography and mass spectrometry was employed to explore the flavor characteristics of different types of GABA tea. We utilized multivariate analyses to identify at least 146 volatile components (VOCs) across 12 functional groups in the GABA tea samples via principal component analysis (PCA).

Epigenetics-targeted drugs: current paradigms and future challenges.

Epigenetics governs a chromatin state regulatory system through five key mechanisms: DNA modification, histone modification, RNA modification, chromatin remodeling, and non-coding RNA regulation. These mechanisms and their associated enzymes convey genetic information independently of DNA base sequences, playing essential roles in organismal development and homeostasis. Conversely, disruptions in epigenetic landscapes critically influence the pathogenesis of various human diseases.

Diagnosis, management and treatment of acquired uterine arteriovenous malformation: A case series and literature review.

The rising prevalence of cesarean sections, abortions, and other surgical interventions involving the uterus has led to an increased incidence of acquired uterine arteriovenous malformations (AVMs). These malformations primarily present as vaginal bleeding, which can significantly impair the quality of life for affected individuals and can pose life-threatening risks in severe instances. Currently, there exists considerable variability in the clinical manifestations, treatment indications, and outcomes associated with uterine AVMs, which substantially complicates clinical diagnosis and management.

Unconventional Near-Equilibrium Nucleation of Graphene on Si-Terminated SiC(0001) Surface.

The transfer-free character of graphene growth on Silicon Carbide (SiC) makes it compatible with state-of-the-art Si semiconductor technologies for directly fabricating high-end electronics. Although significant progress has been achieved in epitaxial growth of graphene on SiC recently, the underlying nucleation mechanism remains elusive. Here, we present a theoretical study to elucidate graphene near-equilibrium nucleation on Si-terminated hexagonal-SiC(0001) surface.

Perihilar cholangiocarcinoma masquerading as intrahepatic cholestasis of pregnancy: a case report and review of the literature.

Cholangiocarcinoma (CCA) is a tumor that arises from the epithelium of the intrahepatic bile ducts. It is rarely diagnosed in individuals under 40 years of age and has a very aggressive nature: 95% of patients die within 5 years. We present the first detailed report of a case of perihilar CCA (pCCA) presenting during pregnancy masquerading as intrahepatic cholestasis of pregnancy (ICP).

Ectopic thyroid follicular carcinoma in the right mandible: a case report.

Background: Ectopic thyroid carcinoma in the mandible is extraordinarily rare; few histologically proven cases have been reported in the literature. Embryologically, cases of ectopic thyroid occur with a developmental abnormality during the migration of the thyroid gland from the floor of the primitive foregut to its final position in the neck. Ectopic thyroid tissue can be found around the course of the thyroglossal duct or laterally in the neck, and even in the mediastinum or below the diaphragm.

Identification of reproduction-related genes in the hypothalamus of sheep (Ovis aries) using the nanopore full-length transcriptome sequencing technology.

The hypothalamus is the coordination center of the sheep (Ovis aries) endocrine system and plays an important role in the reproductive processes of sheep. However, the specific mechanism by which the hypothalamus affects sheep reproductive performance remains unclear. In this study, the hypothalamus tissues of high-reproduction small-tailed Han sheep and low-reproduction Wadi sheep were collected, and full-length transcriptome sequencing by Oxford Nanopore Technologies (ONT) was performed to explore the key functional genes associated with sheep fecundity.

[Research Progress in Magnetic Resonance Imaging of Fetal Ventriculomegaly].

Fetal ventriculomegaly is a central nervous system disorder commonly seen in prenatal imaging, and the prognosis ranges from normal health to severe dysfunction. Currently, fetal predictive markers associated with postpartum individual neurodevelopmental function are still not available, which increases the difficulty of prenatal diagnosis and clinical management. Constant advancements in magnetic resonance imaging (MRI) technology have brought better accuracy and reliability of MRI applied in the diagnosis, prognosis assessment, and etiology investigation of ventriculomegaly.

Effect of meal replacements formula on lipid profiles: A systematic review and meta-analysis of randomized controlled trials.

Background: Although some evidence shows the beneficial effects of meal replacements (MRs) on dyslipidaemias, it is not completely clear. Therefore, we investigate the possible effects of total and partial MRs on lipid profiles.

Methods: For this systematic review and meta-analysis, we searched MEDLINE, Web of Science, SCOPUS, and Embase.

pH monitoring in high ionic concentration environments: performance study of graphene-based sensors.

Graphene-based pH sensors, acclaimed for their exceptional sensitivity to environmental variations, have garnered significant interest in scientific research. However, the sensor performance in high ionic concentration environments is limited, due to the Debye length ion screening effect. In this study, an innovative graphene channel pH sensing device was developed and modified by cross-linked poly(methyl methacrylate) (PMMA).

Proton Pump Inhibitors and Oral-Gut Microbiota: From Mechanism to Clinical Significance.

Proton pump inhibitors (PPIs) are some of the most commonly prescribed drugs worldwide, but there are increasing concerns about digestive complications linked to PPIs. Next-generation sequencing studies have suggested that PPIs can significantly affect the composition of the gut microbiota, which in turn may substantially contribute to the development of these complications. Recently, emerging evidence has suggested that the translocation of oral microbes into the gut may be the primary mechanism underlying the alterations in the gut microbiota induced by PPIs in the presence of gastric acid suppression and impaired oral-gut barrier function.