Journey of the swift nitrogen transformation: Unveiling comammox from discovery to deep understanding.

COMplete AMMonia OXidizer (comammox) refers to microorganisms that have the function of oxidizing NH to NO alone. The discovery of comammox overturned the two-step theory of nitrification in the past century and triggered many important scientific questions about the nitrogen cycle in nature. This comprehensive review delves into the origin and discovery of comammox, providing a detailed account of its detection primers, clades metabolic variations, and environmental factors.

[Effects of conservation tillage on soil microbial community and the function of soil carbon cycling].

Agricultural tillage practices significantly affect the structure and function of soil micro-bial community, as well as its control over soil carbon cycling. Conservation tillage practice based on no-tillage and crop straw returning is an important measure to improve soil carbon sequestration and fertility, in which soil microorganisms play a key role. Although many previous studies focus on the structure and function of microbial communities under conservation tillage, our overall understanding of soil microbial responses at community level upon conservation tillage is still lacking, due to the complexity of the soil, environmental factors and the different selections of microbial research methods.

Anticancer property of Hemp Bioactive Peptides in Hep3B liver cancer cells through Akt/GSK3β/β-catenin signaling pathway.

Foodborne protein hydrolysates exhibit biological activity that may be therapeutic in a number of human disease settings. Hemp peptides (HP) generated by controlled hydrolysis of hemp proteins have a number of health benefits and are of pharmaceutical value. In the present study, we produce small molecular weight HP from hemp seed and investigate its anticancer properties in Hep3B human liver cancer cells.

Long non-coding RNA GAS5 suppresses pancreatic cancer metastasis through modulating miR-32-5p/PTEN axis.

Background: Long non-coding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is a well-known tumor suppressor in the pathogenesis of a variety of human cancers. The precise role of GAS5 in pancreatic cancer (PC) progression is currently unknown, so the aim of this study was to explore the functional participation of GAS5 in PC metastasis.

Methods: The expression changes of GAS5, miR-32-5p and PTEN in human PC specimens and cell lines were compared by means of molecular biology methods.

Long non-coding RNA GAS5 antagonizes the chemoresistance of pancreatic cancer cells through down-regulation of miR-181c-5p.

Objective: To explore the core mechanism of long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) in the regulation of multidrug resistance of pancreatic cancer cells.

Methods: mRNA levels of GAS5, miR-181c-5p and Hippo pathway related genes were detected by quantitative real-time PCR (qRT-PCR). Protein levels of MDR-1, MST1, YAP and TAZ were measured by western blot.

[Characteristics of phosphate-solubilizing microbial community in the soil of poplar plantations under successive-planting and rotation.].

The metagenome sequencing was used to compare the difference of six soil samples, the rhizosphere soil of the first and the second rotation poplar plantations (RSP1, RSP2), the bulk soil of the first and the second rotation poplar plantations (BSP1, BSP2), the soil of rotated peanut field (RPS) and the abandoned land soil (ALS) after poplar clear cutting, in phosphate-solubili-zing microbial community and abundance of phosphatase gene (PG) in a poplar plantation. The results showed that microorganisms from nine genera were related to phosphorus cycle with Bacillus and Pseudomonas being the dominant. The abundance of PSMs was highest in ALS, followed by RPS, RSP2 and BSP2, RSP1 and BSP1, respectively.

[Effects of dexmedetomidine combined with fentanyl in patients undergoing anesthesia induction by sevoflurane].

Objective: To investigate the effects of dexmedetomidine combined with fentanyl in patients undergoing anesthesia induction by sevoflurane.

Method: Eighty patients for elective endotracheal intubation under general anesthesia operations were randomly and double-blindly divided into Dex combined with fentanyl group (Group DF) and the fentanyl group (Group F) from April 2011 to September 2011 at the Fourth Affiliated Hospital of Harbin Medical University, and there were 40 cases in each group. The investigation was approved by all the patients and by the Ethics Committee of the hospital.