[Diagnosis of mycobacterial lymphadenitis by fine needle aspiration cytology and fluorescence quantization-polymerase chain reaction].

Objective: To evaluate the application of fine needle aspiration cytology and fluorescence quantization-polymerase chain reaction (FQ-PCR) in the diagnosis of mycobacterial lymphadenitis.

Methods: Samples obtained by fine needle aspiration cytology (FNAC), which showed granulomatous lesions, from patients with lymph node tuberculosis also confirmed by response to antituberculosis therapy, was subjected to FQ-PCR to test M. tuberculosis DNA (TB-DNA), and the acid-fast bacillus stain.

Novel constructs of tuberculosis gene vaccine and its immune effect on mice.

A novel tuberculosis (TB) gene vaccine containing mouse granulocyte macrophage-colony stimulating factor (mGM-CSF) and a TB antigen (Ag85A) was developed in this study. The genes encoding Ag85A and mGM-CSF were amplified by PCR respectively from the Ag85A-containing pBSby5 and pC-mGM-CSF. The genes were then cloned into two different polylinker sites of plasmid pIRES, forming a novel TB gene vaccine construct pI85AGM.