CD39 expression defines exhausted CD4 T cells associated with poor survival and immune evasion in human gastric cancer.

Objectives: CD4 T cell helper and regulatory function in human cancers has been well characterised. However, the definition of tumor-infiltrating CD4 T cell exhaustion and how it contributes to the immune response and disease progression in human gastric cancer (GC) remain largely unknown.

Methods: A total of 128 GC patients were enrolled in the study.

CD39 identifies an exhausted tumor-reactive CD8 T cell population associated with tumor progression in human gastric cancer.

The ectonucleotidase CD39 has been regarded as a promising immune checkpoint in solid tumors. However, the expression of CD39 by tumor-infiltrating CD8 T cells as well as their potential roles and clinical implications in human gastric cancer (GC) remain largely unknown. Here, we found that GC-infiltrating CD8 T cells contained a fraction of CD39 cells that constituted about 6.

[Alpha-momordicin Regulates Hepatocyte Cytokine Expression].

Objective: To investigate the regulation effect of α-momordicin (α-MMC) on the synthesis and secretion of cytokines in hepatocytes cells.

Methods: Hepatocytes L02 were treated with 189 μg/mL α-MMC with culture supernatant and lysate samples were harvested in different timepoint. Expressions of T-helper 17 (TH17) cytokine profile in samples were detected by the Bio-Plex 200 suspension chip assay system.